Hypomethylating agents plus venetoclax versus intensive chemotherapy prior to transplant in high-risk acute myeloid leukemia

High-risk acute myeloid leukemia (AML) defined by cytogenetics and molecular alterations in the European LeukemiaNetwork 2022 (ELN-2022) classification has a poor prognosis.¹ Allogeneic stem cell transplantation (allo-SCT) in first remission is associated with a significant reduction of relapse risk and improved survival compared to intensive chemotherapy (IC) only.² Older AML patients between 60-74 years of age represent a high-risk population, as they frequently have ELN high-risk AML predicting disease resistance to IC.³ In this age group, IC is associated with low complete remission (CR) rates (approximately 40-50%) and poor overall survival (OS).^1,4

Thus, there is a need for new therapeutic strategies aimed at decreasing toxicities and improving efficacy in this population. The less-intensive regimen combining the hypomethylating agent (HMA) azacitidine (AZA) and the BCL-2 inhibitor venetoclax (VEN) has considerably changed the treatment paradigm for older subjects with newly-diagnosed AML.⁵ Allo-SCT after VEN-AZA appears feasible,⁶ with emerging evidence of post allo-SCT sustained remission.⁷ Yet it still remains an open question whether HMA-VEN is equivalent to IC on post-transplant outcomes in high-risk AML patients.

Therefore, we assembled a retrospective cohort of consecutive patients aged 60-74 years newly diagnosed with ELN-2022 adverse-risk AML¹ treated with allo-SCT after prior intensive or less-intensive remission induction therapy. All patients were diagnosed between April 2014 and May 2024 at three US centers (DFCI, MSKCC, Yale Cancer Center) and five European centers (France: IPC-Marseille; CHU-Nice; HCL-Lyon; CHU-Bordeaux; Lithuania: Vilnius University-Hospital Santaros-Klinikos). Less-intensive treatment consisted of standard HMA-VEN including 7 days of AZA 75 mg/m² or 5-10 days of decitabine (DEC) 20 mg/m². IC induction consisted of conventional 7+3 or CPX-351 plus additional treatment with FLT3-inhibitors per local standards for FLT3-mutated patients. Patients in the IC arm could receive consolidation therapy with Cytarabine per local standards.

Data were collected from patients’ electronic medical records. Institutional review boards of all the participating sites approved the study. We used ELN-2022 response criteria.¹ Composite CR (cCR) was defined as CR + CR with incomplete count recovery (CRi). Overall response (OR) was defined as cCR + morphologic leukemia-free state (MLFS). Time-to-event outcomes were defined from the date of allo-SCT. OS was defined as the time to death from any cause. RFS was the time to either relapse or death. CIR was defined as the time to relapse, with non-relapse mortality (NRM) considered a competing risk. All statistical tests were two-sided and P<0.05 was considered statistically significant. Survival rates were estimated using the Kaplan-Meier method. Log-rank test was used to compare OS and RFS. RI and NRM were compared with Gray’s test. Survival analyses were performed using a Cox proportional hazards regression or a Fine-Gray model in competing risks setting. Multivariable regression models included baseline factors present in >10% of patients and factors significant in univariable analyses (P<0.10).

We included 139 patients with newly diagnosed adverse-risk AML per ELN-2022 who consecutively underwent allo-SCT after first-line therapy between September 2014 and July 2024 (Online Supplementary Figure S1). The median age at diagnosis was 66 years (range, 60-74). Eighty-four patients (60%) had received IC as induction therapy, including 64 (76%) with 7+3 and 20 (24%) with CPX-351 (Table 1). Five patients received FLT3 inhibitors during induction therapy. AML treated with HMA-VEN were more likely to harbor monosomal or complex karyotype (54% vs. 28%; P=0.002), chromosome 5q (del5q, 32% vs. 16%; P=0.032) and 7q deletions (del7q, 28% vs. 9%; P=0.003), or chromosome 17p abnormalities (27% vs. 6%; P=0.001). Twenty-nine patients had TP53 mutations (20.8%), including 23 (16.5%) with a TP53m variant allele frequency (VAF) >10%. HMA-VEN-treated patients had TP53-mutated AML in 32% versus 13% in the IC group (P=0.005).

In the HMA-VEN group, CR, CRi, and MLFS rates were 58.5%, 19%, and 13%, respectively. cCR and OR rates were 77% and 91%. In the IC group, responses were achieved after one (N=53, 83%) or two (N=11, 17%) cycles of induction chemotherapy. CR, CRi, and MLFS rates were 66%, 5%, and 6%, respectively. cCR and OR rates were 71% and 77%. Measurable residual disease (MRD) status at time of best response was available for 68 of 112 patients who achieved OR during induction. In the HMA-VEN group, 22 patients (73%) were MRD negative (MRD⁻), while eight (27%) were MRD postive (MRD⁺). In the IC group, 24 (63%) were MRD⁻ and 14 (37%) MRD⁺.

Seven patients who achieved response relapsed prior to allo-SCT (all in the IC group). When adding patients who never achieved remission, a total of 31 patients were transplanted with active disease, five (9%) in the HMAVEN group and 26 (31%) in the IC group (P=0.003).

With a median follow-up of 52.3 months (95% confidence interval [CI]: 44.4-63.7), post allo-SCT OS was 34.4 (95% CI: 16.1-not reached [NR]) and 28.3 months (95% CI: 14.9-56.3) in the HMA-VEN and IC groups, respectively (P=0.636). RFS was 29.2 (95% CI: 14.7-NR) and 21.1 months (95% CI: 10.7-48.7) in the HMA-VEN and IC groups, respectively (P=0.959; Figure 1A, B). NRM and CIR were similar between both groups (NRM at 6 and 12 months: 8% and 11% in the HMA-VEN group vs. 5% and 18% in the IC group; P=0.632; CIR at 6 and 12 months: 11% and 25% in the HMA-VEN group, 13% and 23% in the IC group; P=0.643). We evaluated survival for patients in cCR at time of allo-SCT and compared it with patients transplanted without achieving cCR. In the HMA-VEN group, post allo-SCT OS was 38.8 (95% CI: 22.2-NR) versus 6.6 months (95% CI: 2.73-NA) respectively (P=0.022). In the IC group, post allo-SCT OS was 48.7 (95% CI: 27.0-NR) versus 12.8 months (range, 8.8-38.7; P=0.010). For these patients, outcome was not impacted by induction type (P=0.55; Figure 1C, D). MRD status was not predictive of post allo-SCT OS or RFS in univariable analysis (hazard ratio [HR]=1.39; 95% CI: 0.70-2.79; P=0.347 and HR=0.81; 95% CI: 0.38-1.73; P=0.583, respectively).

Table 1.Patient and disease characteristics by treatment type.

Figure 1.Overall survival and relapse-free survival in patients treated with allogeneic stem cell transplant after prior induction with hypomethylating agents plus venetoclax or intensive chemotherapy. (A) Comparison of post allogeneic stem cell transplant (allo-SCT) overall survival (OS) for patients treated with either hypomethylating agents plus venetoclax (HMA-VEN) or intensive chemotherapy (IC). (B) Comparison of relapse-free survival (RFS) for patients treated with either HMA-VEN or IC. (C) Comparison of patients in complete response (CR) and CR with incomplete hematological recovery (CRi) with patients in morphologic leukemia free-state (MLFS) and progressive disease (PD) for both treatment type. (D) Comparison of patients in CR-CRi with patients in MLFS after excluding patients treated by allo-SCT with an active disease, for both treatment types.

Multivariable analyses found that induction type (HMA-VEN vs. IC) did not impact post allo-SCT OS (HR=0.95; 95% CI: 0.54-1.68; P=0.864). The only factor associated with better survival was the achievement of cCR at allo-SCT (HR=0.43; 95% CI: 0.26-0.69; P=0.001). Monosomal karyotype was predictive of worse survival (HR=2.29; 95% CI: 1.34-3.92; P=0.003; Table 2). Treatment type did not impact RFS (HR=0.49; 95% CI: 0.22-1.08; P=0.08). Achievement of CR/CRi at allo-SCT was associated with improved RFS (HR=0.29; 95% CI: 0.12-0.72; P=0.0075) while N/KRAS mutation and del7 were associated with worse RFS (HR=2.39; 95% CI: 1.10-5.19; P=0.028 and HR=3.82; 95% CI: 1.68-8.67; P=0.0014, respectively; Online Supplementary Table S1). In exploratory subgroup analyses, patients with AML harboring del5q and TP53 mutations had better OS with HMA-VEN + allo-SCT compared to IC (HR=0.31; 95% CI: 0.12-0.88; P=0.027, and HR=0.39; 95% CI: 0.16-0.98; P=0.044, respectively). When analyzing RFS, we found that in patients with monosomal karyotype (HR=0.31; 95% CI: 0.12-0.78; P=0.013), del5q (HR=0.23; 95% CI: 0.06-0.82; P=0.023), del7q (HR=0.22; 95% CI: 0.06-0.82; P=0.024) or 17p.abn (HR=0.15; 95% CI: 0.03-0.94; P=0.042), HMA-VEN before allo-SCT was associated with prolonged survival compared to IC (Online Supplementary Figure S2).

Allo-SCT after VEN-based treatment is feasible, with low rates of NRM and acceptable OS.^6-8 Recent classifications have demonstrated major differences in the prognostic determinants for intensive and less-intensive therapies.⁹ For example, complex cytogenetics without TP53 mutations, usually associated with resistance to IC, allow for high response rates with HMA-VEN.¹⁰ In multivariable analyses, first-line regimen did not impact OS nor RFS in this cohort of selected patients who underwent allo-SCT. Although no definitive conclusions should be drawn from our subgroup analyses, which are only hypothesis-generating, we found that chromosome 5 and 17p deletions as well as TP53 mutations favored HMA-VEN compared to IC. Although HMA-VEN has yielded poor results in TP53-mutated AML in the VIALE-A cohort,¹¹ most patients were not consolidated with allo-SCT because of age or comorbidities. Interestingly, in a cohort of TP53-mutated AML, a recent report found that induction therapy containing VEN was associated with an increased rate of patients proceeding to allo-SCT, most likely owing to reduced toxicity.¹²

Our study has several limitations. The present cohort was constructed with consecutive patients who reached allo-SCT after either intensive or less-intensive induction, meaning that patients who did not reach transplant in the study period were not included. Therefore, this study does not report the initial number of patients aged 60-74 who began induction with either HMA-VEN or IC. Induction approach was selected by the physician, possibly influenced by patient and disease characteristics. Our dataset did not include information regarding allo-SCT modalities such as conditioning regimen, donor type, stem-cell source or graft-versus-host disease prophylaxis, which could all influence post allo-SCT outcome. Data regarding post allo-SCT maintenance therapy was not available. Additionally, a proportion of patients in the IC cohort were treated before VEN approval.

Table 2.Univariable and multivariable analyses for overall survival.

Despite the limitations, this study is in line with recent evidence showing similar outcomes after initial treatment with HMA-VEN or IC in fit patients, in randomized trials comparing both therapies.¹³ HMA-VEN may allow higher response rates and reduced toxicity, which in turn might improve survival when compared to IC.^7,13,14

Until sufficient follow-up is reached in these studies, real-world data can provide evidence supporting the use of HMA-VEN as an alternative, less toxic induction strategy for patients with ELN-2022 adverse-risk AML who are intended to proceed to allo-SCT.

Footnotes

• Received November 10, 2025
• Accepted March 27, 2026

Correspondence

*GB and JPB contributed equally as first authors.

^#MS and SG contributed euqally as senior authors

S. GARCIAZ - garciazs@ipc.unicancer.fr

M. STAHL - maximilian.stahl@yale.edu

Disclosures

JPB discloses consultancy for GLG, Dedham Group, Guidepoint, Merck, Abbvie, and Rigel. DJA discloses consultancy for Kite, Servier, Incyte, Pfizer, Gilead, Novartis, Jazz, Autolos, Amgen, and Blueprint; honoraria from Amgen and Bristol-Meyers Squibb; research funding from Servier, Novartis, Glycomimetics, AbbVie, and Takeda; data safety monitoring board for MT Sinai MPN Consortium, Fibrogen, and Daiichi-Sankyo. LB discloses consultancy for GLG, Dedham Group, Guidepoint, Merck, Abbvie, and Rigel. RMS discloses consultancy/advisory and honoraria from Servier (also serves on steering committee), Rigel, Kura Oncology, and Gilead Sciences. AZ discloses consultancy and/or honoraria from Pfizer, Astellas, AbbVie, Novartis, and Johnson & Johnson; travel support from AbbVie, Novartis, Johnson & Johnson, and Takeda. RPB served on the steering committee for Servier. LEA discloses consultancy for Cardinal Health, Vaniam Group, and Research To Practice; honoraria from Cardinal Health, Vaniam Group, and DAVA Oncology. RMS discloses research funding from Janssen, and AbbVie; consultancy for Glaxosmithkline, Curis Oncology, Daiichi Sankyo, ENSEM, Epizyme, BerGenBio, AMGEN, Syntrix, Hermavant, Glycomimetrics, CTI Biopharma, Bristol Meyers Squibb, Rigel, Syndax, AvenCell, Takeda, Jazz, Kura Oncology, Lava Therapeutics, Cellarity, Ligand Pharma, Novartis, Aptevo, and Redona Therapeutics; data safety monitoring board for Epizyme, Syntrix, Takeda, and Novartis. MRL discloses honoraria from Pfizer, KITE, Jazz, and AbbVie; research funding from Novartis and AbbVie. JSG discloses research funding from Newave and Taiho; consultancy for Servier, AbbVie, and Genentech; membership on the board of directors or advisory committees for Genentech; research funding from AbbVie and Genentech. ESW discloses consultancy for GLG, Dedham Group, Guidepoint, Merck, Abbvie, and Rigel. ECC discloses consultancy for AbbVie and Rigel. NP discloses consultancy for and honoraria from Blueprint Medicines, Incyte, Novartis, PharmaEssentia, CTI BioPharma/Sobi, Constellation Pharmaceuticals/MorphoSys, AbbVie, Aptose Biosciences, and Karyopharm Therapeutics; honoraria as IDMC Chair from Cogent Biosciences. EMS discloses consultancy/ advisory and consulting fees from AstraZeneca, Servier, Agios Pharmaceuticals, Genentech, Gilead, Jazz Pharmaceuticals, AbbVie, Daiichi Sankyo, Celgene (Bristol Myers Squibb), and Astellas. AMZ discloses consultancy/advisory and/or honoraria from AbbVie, Agios, Akesobio, Amgen, Astellas, BeiGene, BioCryst, Boehringer Ingelheim, Bristol Myers Squibb (including Celgene), Chiesi/Cornerstone Biopharma, Daiichi Sankyo, Dr. Reddy’s Laboratories, Epizyme, Faron, FibroGen, Genentech, Geron, Gilead, GlaxoSmithKline, GlycoMimetics, Janssen, Jasper Therapeutics, Karyopharm Therapeutics, Keros Therapeutics, Kura Oncology, Kyowa Kirin, Lava Therapeutics, Notable, Novartis, Orum, Otsuka, Pfizer, Regeneron, Rigel, Schrödinger, Seagen (formerly Seattle Genetics), Servier, Shattuck Labs, Syndax, Syros, Taiho, Takeda, Treadwell Therapeutics, Vincerx, and Zentalis. EF discloses speaker fees and honoraria for advisory board from Novartis, Sanofi, Gilead, Alexion, MSD, Astellas, Jazz, Pfizer, GSK, and Sobi. TC discloses advisory council or committee for BMS/Celgene, Abbvie, Jazz Pharma, Novartis, Agios, Servier, and BluePrint; has received honoraria from Novartis, Astellas, Celgene/BMS, Jazz Pharma, Servier, and Incyte; and also reports non-financial conflicts with Pfizer, Celgene/BMS, Novartis, Abbvie, Servier, and Gilead. P-YD discloses honoraria and research support to institution from Novartis, Servier, Bristol Myers Squibb, Astellas, and Daiichi-Sankyo; honoraria from AbbVie, Jazz Pharmaceuticals, and Janssen; and research support to institution from Roche. ADG discloses consultancy/advisory roles and/or board/advisory committee memberships with Astellas, Bristol Myers Squibb, Molecular Partners, Syndax, Genentech, AbbVie, and Daiichi Sankyo; consultancy for Ikena Oncology; honoraria from Kura Oncology, and DAVA Oncology; research funding from Aptose, Pfizer, Celularity, Kura Oncology, Aprea, AbbVie, and AROG. MS discloses advisory board for Novartis, Kymera, Sierra Oncology, GSK, Rigel, BMS, Sobi and Syndax, Kura; consulted for Boston Consulting, GLG, and Dedham group; participated in CME activity for Novartis, Curis Oncology, Haymarket Media, and Clinical Care Options; and is member of the medical safety monitoring board for Keros Pharmaceuticals. SG discloses consultancy/advisory and/or honoraria from Janssen, Servier, and AbbVie; consultancy for ImCheck Therapeutics, Sanofi, AbbVie, and Bristol Myers Squibb; travel grants from Sanofi and AbbVie. All other authors have no conflicts of interest to disclose.

Contributions