Chronic myeloid leukemia (CML) is not a public health menace. Despite its rarity, it has, and continues to be, the guiding path for the concept of genetically informed medicine (here you can choose your own favorite alternative catch phrase: bench to bedside medicine, personalized medicine, precision medicine, etc.). CML was the first disease where a specific chromosomal abnormality, the Philadelphia chromosome, was identified, and the first disease where the genetic underpinnings of this chromosome abnormality were discovered (the juxtaposition of portions of the BCR gene from chromosome 22 to the tyrosine kinase domains from chromosome 9).1 This unique BCR-ABL fusion gene drives the pathophysiology of the disease, and thus has led to the remarkable discovery of the tyrosine kinase inhibitors (TKIs), which have fundamentally changed the natural history of the disease. Only decades ago, the lifespan of a chronic phase CML patient was less than seven years while now these patients enjoy a survival roughly that of the normal population.32
Chronic myeloid leukemia has also been the model of disease monitoring using specific molecular markers. In this case, the BCR-ABL chimeric RNA is used to assess disease burden, and the clinical significance of BCR-ABL levels are so compelling as to drive treatment milestones based on BCR-ABL levels that are codified in European and US CML guidelines.64 Here, too, CML has laid the groundwork for other diseases to use so-called minimal (more recently, “measurable”) residual disease (MRD) to drive treatment decisions and measure clinical trial results.
In CML, BCR-ABL is typically measured by testing peripheral blood RNA. RNA is used since the potential breakpoints between BCR and ABL cover many kilobases of DNA sequence, making an easy PCR procedure impossible. Rather, the mRNA species is predictable with only two major splicing variations, making quantitative RT-PCR fairly straightforward. After considerable effort (mostly from the Adelaide group), RNA monitoring has been standardized in an International Scale, making the results comparable across more and more labs worldwide.6 The test is very sensitive, with levels of disease burden usually quantifiable to levels of four to five magnitudes from the standardized IS baseline (where a 4-log reduction of BCR-ABL on the IS would equal 0.01%IS, termed MR4)
However, the RNA assay for BCR-ABL is not perfect. RNA is less stable than DNA, and thus is more susceptible to transit times, temperatures, etc.87 This problem is ameliorated by the use of two control housekeeping genes, but these are subject to the same influences that affect the target gene, and it is perhaps a bit of a leap to believe that a series of environmental insults would affect both target and housekeeping genes in the same way. In addition, there are certainly differences in BCR-ABL levels from patient to patient, if not from cell to cell in the same patient. A DNA-based assay would be a more accurate measure of defining the approximate number of actual CML cells in any given patient. (Note that in rare cases a patient can harbor more than one copy of BCR-ABL, but this is typically only in cases of advanced phase disease.)
Amplification of the BCR-ABL DNA is difficult, as the potential span of breakpoints within the BCR and ABL genes is vast (Figure 1), as opposed to the limited base pair distance once the chimeric BCR-ABL mRNA is assembled.109 To perform the DNA-based assay for BCR-ABL, an initial PCR uses multiple primer sets to first identify the possible DNA breakpoint. Once the sequence of the breakpoint is identified, patient-specific primers are constructed to make a very sensitive assay. Since the patient-specific PCR will have different kinetics from patient to patient, the drop in the disease burden must be measured against the patient’s diagnostic disease burden value. This is a very similar concept to following MRD in patients with acute lymphoblastic leukemia, where patient-specific IgVDJ or TCR rearrangements must be amplified with consensus primers, then patient-specific primers and probes developed for each unique assay. [This complexity led to the development of sensitive flow cytometry and next generation sequencing (NGS) methods, the latter discussed below.]
The potential value of a sensitive DNA test is at least 2-fold. First, one could study the differences in RNA versus DNA load, correlating this with disease response. Secondly, a DNA assay would allow the detection of CML in cases in which the RNA assay of BCR-ABL is undetectable. This would be especially interesting in the case of discontinuation in CML, where some patients with a prolonged deep molecular response stop TKI treatment and do not relapse.1211 Are those cases that quickly relapse after discontinuation simply those in whom the disease is at a higher burden, though undetectable by RNA assays? Could patients who have no MRD by RNA or DNA assays be the lucky patients for whom discontinuation will be successful?13
In this issue of the Journal, the Adelaide group expands on their previous studies of DNA-based BCR-ABL detection and show the potential of this assay to probe basic disease and clinical issues.14 They studied 59 patients with 516 samples on which RNA and DNA assessments of disease burden were performed. Several important findings were found.
First, they found that, early in disease treatment, the number of copies of RNA was generally higher than the DNA (roughly 2-fold) whereas after around six months, RNA and DNA levels were fairly similar. The biological reason for this is unclear. However, the kinetic decay of BCR-ABL with TKI therapy shows a multi-order decay, with an initial decline, followed by a slower decay. These two findings combined suggest that, at diagnosis, a population of committed progenitors, making a fair amount of BCR-ABL RNA (and protein), die quickly, followed by a population of cells less active with regards to BCR-ABL production.
Second, the BCR-ABL decline appears to be more significant in the case of patients with the b14a2 transcript, rather than the shorter b13a2 transcript. This has been seen before in other studies.1615 The reason is unclear, but the speculation is that the exon responsible for the longer version is immunogenetic. CML is well-known to be unusually susceptible to immune-mediated attack (note the effects of interferon, allogeneic transplant, and donor lymphocyte infusions), and the current speculation is that this is another manifestation of this effect.17 If so, we might expect the b14a2 cases to also enjoy more success with discontinuation.
Third, in multiple cases, DNA detected residual disease whereas RNA did not. Thus, in 86 cases where BCR-ABL was undetected by RNA, the DNA assay found disease in 42 (49%). Moreover, the median level of detectable disease after 12 months of therapy was higher by DNA than by RNA.
Where will this lead? First, one could imagine the study of colonial heterogeneity. It is becoming clear that in many diseases (e.g. acute myeloid leukemia) there are multiple related clones at diagnosis, and treatment may cause a Darwinian selection of resistance. Since resistance is less common in CML in the TKI era, and since our tools of defining disease are insensitive to measuring the subtle difference (the Ph and BCR-ABL RNA), DNA-based assays that identify different unique BCR and ABL breakpoints may be able to eventually detect multiple clones. Moreover, DNA-based assays may help distinguish those patients who can and those who should not undergo discontinuation. It may be especially interesting to study cases who have BCR-ABL by DNA but do not subsequently relapse after TKI is discontinued. Is this evidence of immunological control of residual CML? Is this assay detecting BCR-ABL DNA in lymphocytes that may not be involved in the disease process or relapse?
In order to do some of these things, better assays will be needed. The advent of single cell technologies that can perform either genotyping and transcription analysis also need to be developed, and the search for techniques that might allow both to be performed is ongoing.18 This could allow for studies of biology, heterogeneity, and response. Assay methods to quickly genotype complex DNA rearrangement structures are now FDA approved to study immunoglobulin gene rearrangements for MRD in lymphoid malignancies, and the same approach could be used to streamline the DNA approach in CML. New sequencing methods can detect single base pair differences at a one in a million resolution, approximately 3-4 orders of magnitude better than NGS.19
Believers in the theory of the “RNA world” suggest that RNA was the key to life’s first steps from primordial ooze to cellular creatures (alas, some have made it farther than others).20 DNA followed as a more durable way to collect and store information. Perhaps the same evolutionary order is on tap for those researchers interested in the clinical importance of molecular diagnostics in CML.
References
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