AbstractLife-long production of blood from hematopoietic stem cells (HSCs) is a process of strict modulation. Intrinsic and extrinsic signals govern fate options like self-renewal - a cardinal feature of HSCs. Bone morphogenetic proteins (BMP) have an established role in embryonic hematopoiesis, but less is known about its functions in adulthood. Previously, SMAD-mediated BMP signaling has been proven dispensable for HSCs. However, the BMP Type II receptor (BMPR-II) is highly expressed in HSCs, leaving the possibility that BMPs function via alternative pathways. Here, we establish that BMP signaling is required for self-renewal of adult HSCs. Through conditional knockout we show that BMPR-II deficient HSCs have impaired self-renewal and regenerative capacity. BMPR-II deficient cells have reduced p38 activation, implying that non-SMAD pathways operate downstream of BMPs in HSCs. Indeed, a majority of primitive hematopoietic cells do not engage in SMAD-mediated responses downstream of BMPs in vivo. Furthermore, deficiency of BMPR-II results in increased expression of TJP1, a known regulator of self-renewal in other stem cells, and knockdown of TJP1 in primitive hematopoietic cells partly rescues the BMPR-II null phenotype. This suggests TJP1 may be a universal stem cell regulator. In conclusion, BMP signaling, in part mediated through TJP1, is required endogenously by adult HSCs to maintain self-renewal capacity and proper resilience of the hematopoietic system during regeneration.
Hematopoietic stem cells (HSC) have dual capacity to self-renew and give rise to differentiating progeny.1,2 Self-renewal pertains to the ability of HSC to duplicate without losing developmental potential. Maintenance of the stem cell pool is dependent on self-renewal and loss thereof leads to erosion of regenerative capacity and hematopoietic failure. In order to ensure homeostasis, HSC are tightly regulated by internal factors and external signaling cues from the bone marrow (BM) niche.3 Although many regulatory mechanisms have been identified, deeper understanding of the self-renewal machinery is required to fully utilize the therapeutic potential of HSC.
Bone morphogenetic proteins (BMP) belong to the TGF-b superfamily of secreted cytokines, which during embryogenesis regulate a wide variety of biological processes.4-7 Mechanistically, BMP signal through cell surface receptors, which activate receptor-regulated SMAD transcription factors (R-SMAD) through phosphorylation. 8 Phosphorylated R-SMAD form complexes with SMAD4 resulting in nuclear accumulation of activated complexes, which together with cofactors regulate target gene transcription.8 Two classes of receptors have been identified; type-I and type- II. BMP bind to and signal via the BMP type-II receptor (BMPR-II), in association with any type-I receptor (ALK2, ALK3, or ALK6).8
The importance of BMP signaling during development is well established and reflected in early embryonic lethality of mice with targeted deletions of components of the pathway.9-12 Similarly, deletion of SMAD1 and SMAD5 results in embryonic lethality.13-16 Beyond development, the TGF-b superfamily regulates tissue homeostasis and adult regeneration of a variety of organ systems. Several lines of evidence suggest that BMP play a role in adult HSC regulation, but conclusive evidence for direct BMP-requirement by HSC is still lacking. For instance, ALK3-mediated signaling is required by the HSC osteoblastic niche, with loss of ALK3 leading to increased HSC numbers.17 By contrast, decreased levels of BMP4 in the BM results in reduced HSC numbers, as shown in a hypomorphic BMP4 mutant mouse model.18 Additionally, BMP4 maintains cord blood-derived human hematopoietic stem and progenitor cells (HSPC) during ex vivo culture, by acting as a survival factor.19 Recently, Khurana et al. showed that BMP4 exposure in vitro maintains the expression of ITGA4 in murine HSC, thereby preventing culture-induced loss of homing capacity.20 However, SMAD1 and SMAD5 are dispensable for adult HSC, leading to the conclusion that BMP signaling is not endogenously required by adult HSC.21,22 Interestingly, BMPR-II is reportedly highly expressed in adult HSC, suggesting that BMP may signal via alternative circuitries in HSC.23 Indeed, several pathways can be activated by BMP, including components of the MAPK pathway, such as p38 and JNK.24,25 A role for p38 has been suggested in maintenance of ITGA4 expression in HSC in vitro, but a conclusive role for BMP in the regulation of HSC in vivo has never been shown.20 Therefore, in order to investigate the complete role of BMP signaling in HSC in vivo, we conditionally deleted BMPR-II in hematopoietic cells using the Cre/loxP system. We report here that BMPR-II is essential for self-renewal of HSC with mutants displaying significantly reduced regenerative capacity upon BM transplantation. Steady state hematopoiesis is normal in mice deficient of BMPR-II and the differentiation capacity upon transplantation is likewise unaltered, indicating a specific role for BMPR-II in HSC self-renewal. In addition, we map the transcriptional activity of SMAD-mediated signaling in hematopoietic cells by using a BRE-GFP reporter mouse,26 which suggests a failure to engage SMAD-dependent transcriptional response upon BMP exposure. Furthermore, our findings indicate that loss of BMPR-II results in up-regulation of tight junction protein 1 (TJP1) and that knockdown of TJP1 partly rescues the BMPR-II knockout phenotype. TJP1 is a protein previously implicated in self-renewal regulation of several types of stem cells, including both embryonic and adult stem cells. Together, our findings show that BMP signaling, via BMPR-II, is endogenously required by adult HSC to maintain self-renewal capacity in vivo.
Mice on C57Bl/6 background with loxP flanking one allele of exon 4-5 of the BMPR-II gene (MMRRC, University of North Carolina, Chapel Hill, NC, USA)27 were bred to homozygosity and mated with Vav-Cre28 transgenic mice to generate conditional Vav-Cre;BMPR-IIfl/fl mice. Detection of Cre, floxed (fl), wild-type (WT), and excised alleles was done by polymerase chain reaction (PCR) as previously described.22,27 Mice were housed and bred in ventilated cages in the BMC animal facility. All experiments involving animals were approved by the regional Animal Ethical Committee in Lund.
For competitive transplantation assays, 0.2x106 unfractionated BM cells from BMPR-IIfl/fl;Vav-Cre, BMPR-IIfl/+;Vav-Cre, and WT littermates (BMPR-IIfl/fl or BMPR-IIfl/+) (CD45.2) were transplanted with 0.2x106 congenic CD45.1 BM cells by tail vein injection to lethally irradiated (900 cGy) congenic CD45.1/2 recipients (three recipients per donor). Donor, competitor, and recipient cells were monitored by peripheral blood (PB) samplings at several time points at 4-16 weeks. Sixteen weeks post-transplantation mice were killed, BM was analyzed and 2x106 cells were transplanted to secondary recipients, monitored as above. After another 16 weeks secondary mice were killed and tertiary transplantations were performed using 20x106 BM cells. Tertiary recipients were monitored as above for 16 weeks, after which final analyses of BM and PB were performed. For transplantations using purified HSC, ten LSK/CD48-/CD150+ cells from BMPR-IIfl/fl;Vav-Cre or WT littermates were transplanted together with 0.2x106 whole BM support cells (CD45.1/2) to CD45.1 recipients. Reconstitution was monitored as above and BM was analyzed at 16 weeks. Homing assays were performed by transplantation of 15x106 unfractionated BM cells to congenic CD45.1 recipients; BM analysis was done 20 hours post-transplantation. For competitive homing 10x106 BM cells from donors were transplanted with an equal number of WT competitor cells.
Knockdown of TJP1
FFor knockdown of TJP1, lentiviral plasmid pGFP-C-shLenti containing short hairpin RNA (shRNA) targeting TJP1 or scrambled shRNA (OriGene) was used to produce lentiviral particles at the Stem Cell Center Vector Core Facility (Lund University). C-kit-enriched BM cells (CD45.2; BMPR-IIfl/fl;Vav-Cre or WT) were placed into virus-loaded plates at a multiplicity of infection (MOI) of 30-50 and incubated over night (37°C, 5% CO2). Transduced cells were collected and transplanted into lethally irradiated CD45.1 recipient mice (two recipients/donor). An aliquot of cells was cultured for flow cytometry analysis of transduction efficiency after 48 hours. BM of transplanted animals was analyzed at 16 weeks. Additional information can be found in the Online Supplementary Appendix.
BMPR-II is highly expressed in long-term hematopoietic stem cells
In order to map the extent of BMPR-II expression in distinct populations of primitive adult hematopoietic cells, we performed quantitative PCR (qPCR) analyses on sorted long-term HSC (LT-HSC) (LSK-CD34-FLT3-), short-term HSC (ST-HSC) (LSK-CD34+FLT3-), lymphoid-primed multipotent progenitors (LMPP) (LSK-CD34+FLT3+), as well as various progenitor populations.29 Robust expression of BMPR-II was detected in all subsets, although LT-HSC exhibited the highest expression on average between tested populations (Figure 1A). Similarly, we examined expression of type-I receptors ALK2, ALK3, and ALK6 in HSC populations (Figure 1B). In LT- and ST-HSC, both ALK2 and ALK3 were expressed, but expression of both receptors was more abundant in LT-HSC. In LMPP, ALK2 was the dominating receptor. ALK6 was undetectable in all hematopoietic subsets tested.
Normal steady state hematopoiesis despite reduced progenitor activity upon deletion of BMPR-II
Given the robust expression of BMPR-II in LT-HSC, we hypothesized that its deletion would blunt most signaling events initiated by BMP in HSC, allowing us to probe the full role of BMP in adult hematopoiesis. We conditionally deleted BMPR-II in hematopoietic cells, employing the Cre/loxP system with the Vav-Cre driver strain.2728 Efficient deletion of exon 4-5 of the BMPR-II gene in hematopoietic cells was confirmed by PCR analysis of individual colonies from BM, reaching 98.85% efficiency (n=160 alleles; Online Supplementary Figure S1A). Recombination at the BMPR-II locus resulted in efficient reduction of BMPR-II mRNA in purified LT-HSCs (Online Supplementary Figure S1B and C). Vav-Cre mediated deletion in mice homozygous for floxed BMPR-II alleles (BMPR-IIfl/fl;Vav-Cre, hereafter referred to as BMPR-II-/-) did not result in embryonic lethality although the Vav promoter is active from embryonic day (E) 10.5,30 indicating that BMPR-II signaling is not endogenously required in HSC for normal development after E10.5. All PB parameters were normal in adult BMPR-II-/- and BMPRII+/- mice at steady state compared to WT littermates (Figure 2A and B). Similarly, B/T/myeloid lineage distribution and number of cells in the BM of mutant mice were unaltered compared to WT littermates (Figure 2C and data not shown). Megakaryocytic lineage distribution and progenitor populations were also unaltered (Online Supplementary Figure S2A and B). In order to further analyze HSPC lacking BMPR-II, we performed flow cytometry analyses on BM from BMPR-II-/-, BMPR-II+/-, and WT littermate mice. Interestingly, BMPR-II-/- mice had significantly fewer LSK cells in the BM as compared to WT mice (Figure 2D to E). Further analyses by SLAM markers did not reveal significant differences in more primitive subsets of LSK cells, such as LT-HSC (Figure 2D to E). Similarly, when assessing HSC phenotypic aging by CD41 expression31 we saw no significant differences between WT and BMPR-II-/- LT-HSC (Online Supplementary Figure S2C). However, in agreement with the reduced number of LSK cells, the colony forming capacity of BM cells from BMPR-II-/- mice was significantly reduced compared to that of WT littermates (Figure 2F; Online Supplementary Figure 2D), suggesting that primitive hematopoiesis might be altered in BMPR-II-/- mice.
BMPR-II deficient hematopoietic stem cells exhibit reduced regenerative potential upon transplantation
In order to test the regenerative capacity of BMPR-II deficient HSC, we transplanted unfractionated BM cells from BMPR-II-/-, BMPR-II+/-, and WT mice at a 1:1 ratio with congenic WT competitor cells to lethally irradiated recipients (Figure 3A). BMPR-II-/- BM cells exhibited significantly reduced reconstitution capacity in PB short term at 4 weeks (data not shown) and a similar, though non-significant, reduction in PB long term at 16 weeks post-transplantation (Figure 3A to C). Deficiency of BMPR-II did not affect lineage distribution, though a slight decrease in donor contribution to myeloid cells could be observed (Figure 3D). In order to further investigate the ability of BMPR-II-/- cells to contribute to primitive hematopoietic cells, we quantified the number of donor-derived LSK-SLAM cells in BM. Interestingly, BMPR-II-/- cells exhibited a significantly reduced contribution to the entire LSK compartment including all LSK-SLAM populations, including the LT-HSC (LSKCD150+ CD48-) (Figure 3E and F).
Deletion of BMPR-II results in compromised self-renewal capacity and altered long-term hematopoietic stem cell-quality
In order to assay the self-renewal ability of BMPR-II deficient HSC, secondary and tertiary BM transplantations were performed. We transplanted a fixed number of cells from primary recipients to lethally irradiated secondary recipients. Similarly, BM from secondary recipients was transplanted to lethally irradiated tertiary recipients. The overall donor contribution of BMPR-II-/- HSC dropped dramatically upon secondary transplantation, as compared to WT cells, which exhibited stable reconstitution across consecutive transplantations (Figure 3G). Upon tertiary transplantation, BMPR-II-/- cells dropped further, indicating a severely compromised ability to selfrenew under stressed conditions (Figure 3G). BMPR-II+/- BM cells displayed sustained donor contribution in secondary recipients, but appeared to drop upon tertiary transplantation, although not significantly so (Figure 3G). Furthermore, quantification of LT-HSC revealed decreasing numbers of BMPR-II-/- derived cells across consecutive transplantations and in tertiary recipients the contribution to LT-HSC was essentially nonexistent (Figure 3H). These data show that BMPR-II-mediated signaling is essential for self-renewal of LT-HSC in vivo.
In agreement with the in vivo transplantation data stated above is the in vitro serial replating assay which shows a significant decrease in BMPR-II-/- colony number after three platings (Online Supplementary Figure S2E).
In order to verify that the observed defect in regenerative capacity was caused by a qualitative defect of HSC, we transplanted ten sorted BMPR-II-/- or WT LT-HSC in conjunction with congenic WT support BM cells (Figure 3I). In agreement with previous transplantations, overall donor contribution of BMPR-II-/- LT-HSC was significantly reduced at 16 weeks post-transplantation in PB (Figure 3J) and the lineage distribution was unaffected (Figure 3K). Furthermore, the LSK compartment in BM was significantly reduced, as was the CD150-CD48- and CD150- CD48+ subset of LSK cells (Figure 3L). The LT-HSC showed a similar reduction, though it did not reach significance (P=0.09) (Figure 3L).
Loss of BMPR-II causes transcriptional cell cycle perturbation but has little or no effect on cell cycle and apoptosis in long-term hematopoietic stem cells
In order to investigate the biological properties of BMPR-II-/- primitive hematopoietic cells, we analyzed apoptosis and cell cycle parameters of BM cells from BMPR-II-/- and WT mice by flow cytometry. The fraction of apoptotic (AnnexinV+) cells within LSK/LSK-SLAM populations did not differ between BMPR-II-/- and WT BM (Figure 4A and B). Cell cycle distribution, analyzed using Ki67 and DAPI, was mostly unaltered in all hematopoietic populations tested between BMPR-II-/- and controls (Figure 4C). We observed a slight decrease in quiescent G0-phase LT-HSC and a slight increase in LT-HSC in G1-phase, though these differences did not reach significance (Figure 4D). Similar results were seen in other primitive hematopoietic populations, with a significant decrease of cells in G0 in the CD150-CD48- and CD150- CD48+ subsets of LSK cells (Online Supplementary Figure S3A to C). In contrast to the lack of significant cell cycle perturbation in LT-HSC was the observed enrichment in gene sets pertaining to cell cycling (Online Supplementary Figure S4A). When the hematopoietic system was put under stress following in vivo treatment with 5-fluorouracil, the blood, BM, and spleen were mostly unaffected. Even though white blood cells and splenic LT-HSC were reduced, this was not significant (Online Supplementary Figure S5A to D). Furthermore, the proliferative capacity of BMPR-II-/- c-kit+ BM cells in vitro was normal when assayed under serum-free conditions in the presence of SCF, IL-3, and IL-6 (Figure 4E).
Homing is unaffected by deletion of BMPR-II
As BMP signaling has been linked to HSC homing via maintenance of ITGA4 expression during ex vivo culture, we investigated if loss of BMPR-II resulted in a homing defect.20 We transplanted unfractionated BMPR-II-/- and WT BM cells, with or without competitor cells, to lethally irradiated recipients. Following 20 hours, BM was analyzed by flow cytometry. The donor contribution to Lin- Sca1+CD150+ cells as well as to the overall Lin- population was not significantly altered between BMPR-II-/- and WT cells (Figure 4F; Online Supplementary Figure S6A). Donor contribution following competitive transplantation was also not significantly altered (Online Supplementary Figure S6B to C). Likewise, the expression of ITGA4 (CD49d) was unaltered between BMPR-II-/- and control LT-HSC, indicating that BMP signaling does not regulate ITGA4 expression in vivo (Online Supplementary Figure S6D).
Reduced phosphorylation of SMAD1 upon BMPR-II deletion
In order to investigate the SMAD signaling status of BMPR-II-/- hematopoietic cells, we performed western blots of purified c-kit+ cells incubated with/without BMP4 in vitro. As expected, BMPR-II-/- cells exhibited significantly reduced phosphorylated SMAD1/5, both in the presence and absence of BMP4 stimulation (Figure 5A). WT cells exhibited robust levels of phosphorylated SMAD1/5, but the level was not further increased upon BMP4 exposure, suggesting already saturated levels. These data confirm that deletion of BMPR-II translates into a functional reduction of SMAD signaling.
Limited SMAD-dependent transcriptional activity in hematopoietic populations
Although SMAD1/5-mediated BMP signaling is dispensable for HSC function, transcriptional activity of SMAD downstream of BMP has not been characterized in detail in hematopoietic cells. Using a BRE-GFP reporter mouse, a well-established model for gauging in vivo transcriptional activity of SMAD1/5/8,26,32-34 cells responding transcriptionally to BMP through SMAD were measured by green fluorescent protein (GFP), allowing in vivo analysis. BRE-GFP BM cells displayed limited activation of the SMAD pathway, with the highest proportion of GFP+ cells reaching only 2.79 % on average (LSK CD150-CD48- population) (Figure 5B). In order to investigate whether hematopoietic cells could respond to BMP signaling via the SMAD pathway, BRE-GFP cells were stimulated in vitro for 16 hours with/without BMP4. No significant difference in GFP+ cells was found in any BM population (Figure 5C).
Loss of BMPR-II results in a reduction of p38
As p38 has been implicated downstream of BMP in hematopoietic cells, we evaluated the level of phosphorylated p38 in c-kit+ progenitor cells by western blot. Phospho-p38 was reduced in un-stimulated BMPR-II-/- cells, though it did not reach significance, and its level did not change following stimulation with BMP4 (Figure 5D). We could not detect a robust increase of phospho-p38 in BMP4-stimulated WT cells. Instead, phospho-p38 was reduced following BMP4 stimulation in WT c-kit+ cells (Figure 5D). Additionally, the reduction of phospho-p38 in BMPR-II-/- cells may be a reflection of significantly reduced total p38 (Figure 5E). We found no significant differences in protein levels of other known signaling mediators such as phospho-Limk, phospho-Cofilin, or RhoA/B (Online Supplementary Figure S7A and B). In order to further investigate whether the reduction in LSK cell numbers in BMPR-II null mice is related to known downstream BMP signaling mediators such as the MAPK pathway, gene expression was evaluated in sorted LSK cells. No significant differences were found among the investigated genes (Online Supplementary Figure S7C). Finally, we assessed expression levels of BMP type-I and other type- II receptors in sorted WT and BMPR-II-/- primitive hematopoietic cells (LSK CD48-) to determine whether BMPR-II deletion leads to up- or down-regulation of other BMP receptors. We found no significant differences, despite a trend of increased Alk3 in the absence of BMPRII (Online Supplementary Figure S7D to E).
Deficiency of BMPR-II results in up-regulation of TJP1 in long-term hematopoietic stem cells
In order to further explore underlying mechanisms behind the BMPR-II-/- phenotype, we performed microarray analysis on highly purified LT-HSC (LSKCD150+ CD48-CD9hi)35 from adult mice. The analysis generated a number of differentially expressed genes (Online Supplementary Figures S8 and S4B) and enriched gene sets (Online Supplementary Figure S4A). Selected genes, based on relevant known connections to stem cell function, hematopoiesis or BMP, were further validated. qPCR analyses confirmed a significant 2.4-fold up-regulation of TJP1 in BMPR-II-/- LT-HSC (Figure 5F).
In order to further investigate whether the reduction in LSK cell numbers in BMPR-II null mice is related to factors known to associate with TJP1 such as SRC and STAT3, gene expression was evaluated in sorted LSK cells. No significant differences were found among the investigated genes (Online Supplementary Figure S7C). We also found no significant differences in expression of Alpk or microRNA 15a/23b/27a, which were other hits in the array (Online Supplementary Figure S7F and G).
TJP1 knockdown partly rescues the BMPR-II knockout phenotype
In order to evaluate the contribution of TJP1 up-regulation to the observed BMPR-II-/- phenotype, TJP1 knockdown was performed using shRNA lentiviral vectors in ckit-enriched BM cells from BMPR-II-/- and WT mice. Transduced cells were transplanted to WT recipients. Using shRNA-C knockdown of TJP1 was achieved to at least 0.51-fold level (compared to un-transduced cells) (Online Supplementary Figure 9A). Average transduction efficiency at transplantation was 36 % and 33 % for scrambled- shRNA transduced WT and BMPR-II-/- groups respectively; 21 % and 26 % for TJP1-shRNA transduced WT and BMPR-II-/- groups (Online Supplementary Figure S9B).
In transplanted mouse BM the donor LSK compartment showed a partial rescue, as TJP1-shRNA transduced BMPR-II-/- cells no longer showed reduced engraftment in comparison to Scrambled-shRNA transduced WT cells (Figure 6A). A trend of increased engraftment was seen among HSC, although this did not reach significance (Figure 6B). In hierarchically lower populations no similar effect on engraftment was seen (Figure 6C and D), nor in PB (data not shown).
A large body of work from a variety of model systems has established a critical role for BMP signaling during early development.5-7 Studies performed in vitro indicate that BMP signaling continues to function in the regulation of HSC beyond development.19,20,36 However, SMAD1 and SMAD5 are dispensable for adult HSC function in mice, leading to the conclusion that BMP play a limited role, if any, in adult HSC regulation in vivo.21,22 The SMAD circuitry is undoubtedly the best characterized pathway downstream of BMP, but the lack of HSC phenotype in mice deficient of SMAD1 and SMAD5 does not automatically rule out a role for BMP signaling in adult HSC, as non- SMAD pathways can also be activated by BMP.24,25 The fact that BMPR-II is highly expressed in LT-HSC has left a gap in knowledge between the BMP circuitry and its function in adult HSC in vivo.23
Here we aimed to elucidate the endogenous role of BMP signaling in adult murine HSC, by conditional deletion of BMPR-II specifically in hematopoietic cells. Unlike deletion of SMAD1 and SMAD5, we report here that BMPR-II is essential for self-renewal of adult HSC. It is likely that this non-SMAD signal in HSC is mediated by BMPR-II associated with the BMP type-I receptor ALK2 or possibly ALK3, based on our transcriptional profiling of receptor expression in WT LT-HSC and that we find no significant change in expression levels of other BMP receptors in primitive hematopoietic cells from BMPR-II-/- mice. Additionally, there is limited knowledge about BMPR-II being able to activate downstream signaling pathways independently of type-I receptors. Despite the absence of significant differences in our measurements of above mentioned transcript levels, a trend of increased Alk3 seemed to be observed following BMPR-II deletion. This will require further studies to fully decipher the relation between BMP receptors and their cross-regulation, and to understand their relative function in the context of HSC regulation. Though it is possible that cross-talk and feedback regulation occurs within the BMP signaling pathway, BMPR-II deletion does not seem to have a regulatory effect at transcript level on other BMP family receptors in HSC.
In this study we show that BMPR-II deficient HSC fail to efficiently generate additional HSC upon transplantation, thus causing a significant reduction in hematopoietic regeneration following serial BM transplantation. Loss of BMPR-II did not affect homing capacity of HSC to the BM, suggesting that the reduced regenerative capacity observed upon transplantation derives from compromised self-renewal ability of LT-HSC. During steady state hematopoiesis, BMPR-II-/- mice display essentially normal hematopoietic parameters, lending further evidence to a specific role for BMPR-II in self-renewal of LT-HSC. Furthermore, as cell cycle distribution among LT-HSC is more or less unaffected by loss of BMPR-II and the hematopoietic system recovers almost normally following stress, our data suggest that a possible effect on cell cycle progression plays only a small part in HSC regulation by BMP. Instead, LT-HSC deficient of BMPR-II fail to maintain stemness during conditions when self-renewal divisions are required. This is in agreement with previous data, which shows that BMP stimulation does not affect proliferation of HSC in vitro.23
By investigating the transcriptional activity of the SMAD pathway, our data reveals that a majority of hematopoietic cells fail to respond transcriptionally to BMP and thus do not employ SMAD-dependent transcriptional response, despite phosphorylation of SMAD. We hypothesize that other regulatory mechanisms limit the ability of the SMAD pathway to engage transcriptionally in response to BMP stimulation and that BMP preferentially signal through non-SMAD circuitries in hematopoietic cells. The BRE-reporter study is in agreement with the lack of hematopoietic phenotype seen upon deletion of SMAD1/SMAD5.
The p38 signaling pathway is an alternative signaling circuitry implicated downstream BMP receptors. Khurana et al. showed that p38 is phosphorylated in both human and mouse HSPC cultured in the presence of BMP4 in vitro.20 In agreement with this, we observed a reduction in phosphorylation of p38 in hematopoietic progenitor cells lacking BMPR-II. However, we could not detect a robust induction of phosphorylation in response to BMP4 in WT progenitor cells. This may be due to the length of BMP stimulation, as Khurana et al. measured p38 signaling following 5 days of continuous BMP4 exposure. We assayed p38 after 30 minutes of BMP4 stimulation, a time point to measure direct activation. Reduced phosphorylation of p38 is therefore in agreement with a more long-term loss of BMPR-II, and may thus be due to secondary effects.
Interestingly, we observed a significant increase in expression of TJP1 in purified BMPR-II-/- LT-HSC. TJP1 has previously been linked to regulation of self-renewal in embryonic stem cells where loss of TJP1 results in increased self-renewal.37 Expression of TJP1 is shared between HSC, ES cells, and neural stem cells, indicative of a universal role for TJP1 in self-renewal of stem cells.38 Additionally, TJP1 is downregulated in a multipotent hematopoietic cell line upon differentiation.39 Taken together, these data substantiate the link between TJP1 and HSC self-renewal. Contrary to what is seen in hematopoietic cells in vitro,39 our data suggests that loss of BMPR-II leads to disruption of HSC self-renewal via excessive expression of TJP1, which is in line with previous findings in ES cells.37 It is possible that fine-tuned HSC regulation in vivo requires very specific levels of TJP1. Our findings further show that knockdown of TJP1 partly rescues the BMPR-II null phenotype. Following transplantation of BMPR-II-/- cells with TJP1 knockdown, we observed an increase in cell contribution to the donor LSK compartment. A similar trend was seen in the HSC compartment, but not in more differentiated populations. Our data suggests that the up-regulation of TJP1 is, at least in part, one of the key mechanisms behind the observed BMPR-II-/- hematopoietic phenotype. Complete reversal of the phenotype may not have been achieved due to incomplete knockdown or that in addition to TJP1 there could be other mechanisms playing a part in generating the phenotype.
In order to increase the therapeutic applicability of HSC, more detailed information is required regarding mechanisms controlling fate options such as self-renewal. In human hematopoiesis BMP have been shown to have an important role in adhesion to stroma, differentiation potential and ex vivo maintenance.19,20,36 Here, we identify BMPR-II and TJP1 as important players regulating murine LT-HSC self-renewal in vivo. In light of our findings, further work should focus on investigating the role for BMPR-II and in particular TJP1 in human HSC self-renewal.
- Received August 28, 2019
- Accepted July 10, 2020
No conflicts of interest to disclose.
SW, UB, and SK designed experiments; SW, UB, MD, THMG, LS, and SA performed experiments; SW and UB analysed data. SW, UB, and SK wrote the paper; SK supervised the study.
This work was supported by funds from the European Commission (Stemexpand); Hemato-Linné and Stemtherapy program project grants from the Swedish Research Council; a project grant to SK from the Swedish Research Council; the Swedish Cancer Society; the Swedish Childhood Cancer Fund; a Clinical Research Award from Lund University Hospital; and a grant to SK from The Tobias Foundation awarded by the Royal Academy of Sciences.
The authors would like to thank Leif Oxburgh from the Maine Medical Center for support in initial BRE-LacZ analyses, Elaine Dzierzak from the University of Edinburgh for providing BRE-GFP reporter mice, Shamit Soneji and Stefan Lang at the Stem Cell Center Bioinformatics Core Facility at Lund University for input on the microarray and advice on statistical analyses, and Göran Karlsson at Lund University for continuous feedback on the project and manuscript.
- Ogawa M. Differentiation and proliferation of hematopoietic stem cells. Blood. 1993; 81(11):2844-2853. https://doi.org/10.1182/blood.V81.11.2844.2844Google Scholar
- Orkin SH, Zon LI. Hematopoiesis: an evolving paradigm for stem cell biology. Cell. 2008; 132(4):631-644. https://doi.org/10.1016/j.cell.2008.01.025Google Scholar
- Zon LI. Intrinsic and extrinsic control of haematopoietic stem-cell self-renewal. Nature. 2008; 453(7193):306-313. https://doi.org/10.1038/nature07038Google Scholar
- Massague J. TGFbeta signalling in context. Nature Rev M Cell Biol. 2012; 13(10):616-630. https://doi.org/10.1038/nrm3434Google Scholar
- Sadlon TJ, Lewis ID, D'Andrea RJ. BMP4: its role in development of the hematopoietic system and potential as a hematopoietic growth factor. Stem Cells. 2004; 22(4):457-474. https://doi.org/10.1634/stemcells.22-4-457Google Scholar
- Snyder A, Fraser ST, Baron MH. Bone morphogenetic proteins in vertebrate hematopoietic development. J Cell Biochem. 2004; 93(2):224-232. https://doi.org/10.1002/jcb.20191Google Scholar
- Larsson J, Karlsson S.. The role of Smad signaling in hematopoiesis. Oncogene. 2005; 24(37):5676-5692. https://doi.org/10.1038/sj.onc.1208920Google Scholar
- Massague J. TGF-beta signal transduction. Annu Rev Biochem. 1998; 67:753-791. https://doi.org/10.1146/annurev.biochem.67.1.753Google Scholar
- Mishina Y, Suzuki A, Ueno N, Behringer RR. Bmpr encodes a type I bone morphogenetic protein receptor that is essential for gastrulation during mouse embryogenesis. Genes Dev. 1995; 9(24):3027-3037. https://doi.org/10.1101/gad.9.24.3027Google Scholar
- Winnier G, Blessing M, Labosky PA, Hogan BL. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev. 1995; 9(17):2105-2116. https://doi.org/10.1101/gad.9.17.2105Google Scholar
- Beppu H, Kawabata M, Hamamoto T. BMP type II receptor is required for gastrulation and early development of mouse embryos. Dev Biol. 2000; 221(1):249-258. https://doi.org/10.1006/dbio.2000.9670Google Scholar
- Zhang H, Bradley A.. Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development. Development. 1996; 122(10):2977-2986. https://doi.org/10.1242/dev.122.10.2977Google Scholar
- Lechleider RJ, Ryan JL, Garrett L. Targeted mutagenesis of Smad1 reveals an essential role in chorioallantoic fusion. Dev Biol. 2001; 240(1):157-167. https://doi.org/10.1006/dbio.2001.0469Google Scholar
- Tremblay KD, Dunn NR, Robertson EJ. Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001; 128(18):3609-3621. https://doi.org/10.1242/dev.128.18.3609Google Scholar
- Chang H, Huylebroeck D, Verschueren K, Guo Q, Matzuk MM, Zwijsen A.. Smad5 knockout mice die at mid-gestation due to multiple embryonic and extraembryonic defects. Development. 1999; 126(8):1631-1642. https://doi.org/10.1242/dev.126.8.1631Google Scholar
- Yang X, Castilla LH, Xu X. Angiogenesis defects and mesenchymal apoptosis in mice lacking SMAD5. Development. 1999; 126(8):1571-1580. https://doi.org/10.1242/dev.126.8.1571Google Scholar
- Zhang J, Niu C, Ye L. Identification of the haematopoietic stem cell niche and control of the niche size. Nature. 2003; 425(6960):836-841. https://doi.org/10.1038/nature02041Google Scholar
- Goldman DC, Bailey AS, Pfaffle DL, Al Masri A, Christian JL, Fleming WH. BMP4 regulates the hematopoietic stem cell niche. Blood. 2009; 114(20):4393-4401. https://doi.org/10.1182/blood-2009-02-206433Google Scholar
- Bhatia M, Bonnet D, Wu D. Bone morphogenetic proteins regulate the developmental program of human hematopoietic stem cells. J Exp Med. 1999; 189(7):1139-1148. https://doi.org/10.1084/jem.189.7.1139Google Scholar
- Khurana S, Buckley S, Schouteden S. A novel role of BMP4 in adult hematopoietic stem and progenitor cell homing via Smad independent regulation of integrinalpha4 expression. Blood. 2013; 121(5):781-790. https://doi.org/10.1182/blood-2012-07-446443Google Scholar
- Singbrant S, Karlsson G, Ehinger M. Canonical BMP signaling is dispensable for hematopoietic stem cell function in both adult and fetal liver hematopoiesis, but essential to preserve colon architecture. Blood. 2010; 115(23):4689-4698. https://doi.org/10.1182/blood-2009-05-220988Google Scholar
- Singbrant S, Moody JL, Blank U. Smad5 is dispensable for adult murine hematopoiesis. Blood. 2006; 108(12):3707-3712. https://doi.org/10.1182/blood-2006-02-003384Google Scholar
- Utsugisawa T, Moody JL, Aspling M, Nilsson E, Carlsson L, Karlsson S.. A road map toward defining the role of Smad signaling in hematopoietic stem cells. Stem Cells. 2006; 24(4):1128-1136. https://doi.org/10.1634/stemcells.2005-0263Google Scholar
- Blank U, Brown A, Adams DC, Karolak MJ, Oxburgh L.. BMP7 promotes proliferation of nephron progenitor cells via a JNKdependent mechanism. Development. 2009; 136(21):3557-3566. https://doi.org/10.1242/dev.036335Google Scholar
- Derynck R, Zhang YE. Smad-dependent and Smad-independent pathways in TGFbeta family signalling. Nature. 2003; 425(6958):577-584. https://doi.org/10.1038/nature02006Google Scholar
- Monteiro RM, de Sousa Lopes SM, Bialecka M, de Boer S, Zwijsen A, Mummery CL. Real time monitoring of BMP Smads transcriptional activity during mouse development. Genesis. 2008; 46(7):335-346. https://doi.org/10.1002/dvg.20402Google Scholar
- Beppu H, Lei H, Bloch KD, Li E.. Generation of a floxed allele of the mouse BMP type II receptor gene. Genesis. 2005; 41(3):133-137. https://doi.org/10.1002/gene.20099Google Scholar
- Stadtfeld M, Graf T.. Assessing the role of hematopoietic plasticity for endothelial and hepatocyte development by non-invasive lineage tracing. Development. 2005; 132(1):203-213. https://doi.org/10.1242/dev.01558Google Scholar
- Pronk CJ, Rossi DJ, Mansson R. Elucidation of the phenotypic, functional, and molecular topography of a myeloerythroid progenitor cell hierarchy. Cell Stem Cell. 2007; 1(4):428-442. https://doi.org/10.1016/j.stem.2007.07.005Google Scholar
- Chen MJ, Yokomizo T, Zeigler BM, Dzierzak E, Speck NA. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature. 2009; 457(7231):887-891. https://doi.org/10.1038/nature07619Google Scholar
- Gekas C, Graf T.. CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age. Blood. 2013; 121(22):4463-4472. https://doi.org/10.1182/blood-2012-09-457929Google Scholar
- Blank U, Seto ML, Adams DC, Wojchowski DM, Karolak MJ, Oxburgh L.. An in vivo reporter of BMP signaling in organogenesis reveals targets in the developing kidney. BMC Dev Biol. 2008; 8:86. https://doi.org/10.1186/1471-213X-8-86Google Scholar
- Crisan M, Kartalaei PS, Vink CS. BMP signalling differentially regulates distinct haematopoietic stem cell types. Nat Commun. 2015; 6:8040. https://doi.org/10.1038/ncomms9040Google Scholar
- Crisan M, Solaimani Kartalaei P, Neagu A. BMP and hedgehog regulate distinct AGM hematopoietic stem cells ex vivo. Stem Cell Reports. 2016; 6(3):383-395. https://doi.org/10.1016/j.stemcr.2016.01.016Google Scholar
- Karlsson G, Rorby E, Pina C. The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells. Cell Rep. 2013; 4(4):642-648. https://doi.org/10.1016/j.celrep.2013.07.020Google Scholar
- Jeanpierre S, Nicolini FE, Kaniewski B. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood. 2008; 112(8):3154-3163. https://doi.org/10.1182/blood-2008-03-145326Google Scholar
- Xu J, Lim SB, Ng MY. ZO-1 regulates Erk, Smad1/5/8, Smad2, and RhoA activities to modulate self-renewal and differentiation of mouse embryonic stem cells. Stem Cells. 2012; 30(9):1885-1900. https://doi.org/10.1002/stem.1172Google Scholar
- Ramalho-Santos M, Yoon S, Matsuzaki Y, Mulligan RC, Melton DA. "Stemness": transcriptional profiling of embryonic and adult stem cells. Science. 2002; 298(5593):597-600. https://doi.org/10.1126/science.1072530Google Scholar
- Bruno L, Hoffmann R, McBlane F. Molecular signatures of self-renewal, differentiation, and lineage choice in multipotential hemopoietic progenitor cells in vitro. Mol Cell Biol. 2004; 24(2):741-756. https://doi.org/10.1128/MCB.24.2.741-756.2004Google Scholar
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