Sialic acid is a common terminal residue of glycans on proteins and acidic sphingolipids such as gangliosides with important biological functions. The sialylation process is controlled by more than 20 different sialyltransferases, many of which exhibit overlapping functions. Thus, it is difficult to determine the overall biological function of sialylation by targeted deletion of individual sialyltransferase. To address this question, we established a mouse line with the Slc35a1 gene flanked by loxP sites. Slc35a1 encodes the CMP-sialic acid transporter that transports CMP-sialic acid from cytoplasm into the Golgi apparatus for sialylation. Here we report our study regarding the role of sialylation on megakaryocytes and platelets using a mouse line with significantly reduced sialylation in megakaryocytes and platelets (Plt Slc35a1-/-). The major phenotype of Plt Slc35a1-/- mice was thrombocytopenia. The number of bone marrow megakaryocytes in Plt Slc35a1-/- mice was reduced, and megakaryocyte maturation was also impaired. In addition, an increased number of desialylated platelets was cleared by Kupffer cells in the liver of Plt Slc35a1-/- mice. This study provides new insights into the role of sialylation in platelet homeostasis and the mechanisms of thrombocytopenia in diseases associated with platelet desialylation, such as immune thrombocytopenic purpura and a rare congenital disorder of glycosylation (CDG), SLC35A1-CDG, which is caused by SLC35A1 mutations.
Platelets are among the most abundant blood cells in circulation. In addition to their well-established roles in hemostasis and thrombosis, platelets are involved in a broad spectrum of other physiological and pathological processes such as vascular integrity, immunity, inflammation, and tumor metastasis.1-5 A stable number of platelets is of great importance to these functions. Platelet homeostasis is primarily regulated by the production and clearance of platelets. Platelet membrane receptors play a vital role in platelet hemostasis. Most of these proteins are glycoproteins (GP), including the GPIb-IX-V complex and GPIIb/IIIa complex.6 Major forms of glycosylation include N-linked glycans (N-glycans) and mucin-type O-linked glycans (O-glycans).7 Both N- and O-glycans are commonly “capped” by sialic acids, a process named sialylation.8,9 Sialylation plays essential biological roles, especially in cell-cell interactions.8 The significance of sialylation in other biological processes, such as platelet homeostasis, has just begun to be appreciated.
α2,3-linked sialic acid is the main form of platelet sialylation and is commonly linked to the penultimate galactose (Gal) or N-acetylgalactosamine (GalNAc) on complex N-glycans and O-glycans.7 The sialylation level on platelets is reduced in conditions such as cold storage of platelets, sepsis, and a subset of immune thrombocytopenia. 10,11 It has been reported that desialylated platelets express terminal galactose and are cleared by hepatocytes through interactions with hepatic asialoglycoprotein receptor.12-14 This mechanism is considered to regulate normal platelet homeostasis and contributes to thrombocytopenia under pathological conditions. Our recent study revealed that Küpffer cells, rather than hepatocytes, phagocytose desialylated or O-glycan-deficient platelets.7 Glycosylation is also important for megakaryocytopoiesis and platelet production. Mice with constitutive or inducible global loss of O-glycans exhibited thrombocytopenia due to defects in terminal megakaryocyte differentiation and platelet production, demonstrating that Oglycosylation is critical for thrombocytogenesis.15,16
Sialylation occurs in the trans-Golgi by transferring sialic acids from the donor substrate cytidine-5’-monophosphate- sialic acid (CMP-SA) to acceptor N- and/or O-glycans (Figure 1A).8,9 There are de novo and salvage pathways of CMP-SA biosynthesis. In the de novo pathway, sialic acids are synthesized in the cytoplasm from the substrate uridine diphosphate (UDP)-N-acetylglucosamine through serial reactions catalyzed by the enzymes UDP-N-acetylglucosamine 2-epimerase, N-acetylneuraminic acid synthase, and N-acetylneuraminate-9-phosphate-phosphatase. On the other hand, sialic acid can also be generated during desialylation of sialylated molecules. Sialic acids are attached to CMP catalyzed by CMP-SA synthase in the nucleus, and subsequently CMP-SA is transported into the Golgi lumen by the CMP-sialic acid transporter SLC35A1.
There are more than 20 known genes in mice and humans encoding sialyltransferases. α2,3-linked sialic acids on glycans of megakaryocytes and platelets are generated by six different α2,3-sialyltransferases (ST3Gal-I – VI). Studies with mice lacking ST3Gal-I or ST3Gal-IV have shown the importance of sialylation in platelet biology, especially platelet clearance by hepatocytes and/or Küpffer cells.17 However, due to redundant expression of these sialyltransferases in platelets, such as ST3Gal-I, ST3Gal-IV, and ST6Gal-I,18,19 the overall function of sialylation in platelet homeostasis remains to be studied.
The CMP-SA transporter (CST) is encoded by the SLC35A1 gene (Figure 1A). Several patients with SLC35A1 mutations have been reported and are described as having an SLC35A1-related congenital disorder of glycosylation (SLC35A1-CDG). Clinical manifestations in these rare SLC35A1-CDG patients are complex, including neutropenia, opportunistic infections, delayed psychomotor development, epilepsy, ataxia, microcephaly and choreiform.20-23 However, a common manifestation in these patients is macrothrombocytopenia. Accelerated platelet clearance, but not impaired proplatelet production, is considered to contribute to the thrombocytopenia based on in vitro analyses.23
CST is a critical transporter to exchange CMP-sialic acid for CMP across the Golgi membrane, which is essential for sialylation, using an antiport mechanism (Figure 1A).24 So far, no mouse model with deficiency of CMP-SA is available to study this key process. For this reason, we generated genetically-targeted mice with exons 3 and 4 of Slc35a1 being flanked by loxP sites (Slc35a1f/f). In this study, we established a mouse model lacking Slc35a1 specifically in megakaryocytes and platelets (Plt Slc35a1–/–) to determine whether desialylation affects platelet homeo stasis. Plt Slc35a1–/– mice exhibited thrombocytopenia with impaired megakaryocyte maturation and increased platelet clearance. This study provides new insights into the role of sialylation in platelet production and clearance.
Based on our published methods,7,25 we first generated conditional mice with the Slc35a1 gene flanked by loxP sites (Slc35a1f/f). Plt Slc35a1–/– mice were generated by crossing Slc35a1f/f mice with a transgenic mouse line expressing Cre recombinase under the control of the mouse Pf4 promoter (Pf4Cre mice, The Jackson Laboratory, #008535) (Figure 1B). Mice were bred and maintained in specific pathogen-free conditions in the Laboratory Animal Experimental Center at Soochow University and Oklahoma Medical Research Foundation. Mouse studies were approved by the Animal Use Committee of the First Affiliated Hospital of Soochow University and Oklahoma Medical Research Foundation.
Platelet preparation and analysis
Platelets were isolated and prepared based on our previous publications. 3,7,26 In brief, whole blood was obtained from the inferior vena cava and collected into test-tubes containing sodium citrate as an anticoagulant, diluted 1:1 with Tyrode buffer, and centrifuged at 100 g for 10 min at room temperature. The supernatant was collected and centrifuged at 800 g for 8 min to obtain the platelet pellet, followed by two subsequent washes with Tyrode buffer. Details are provided in the Online Supplementary Data.
Details of flow cytometry analysis of platelet sialylation profile, reticulated platelets, and glycoprotein expression on platelet surfaces are provided in the Online Supplementary Data.
Analysis of bone marrow megakaryocytes
Purification of megakaryocytes from the bone marrow, megakaryocyte counts of bone marrow paraffin-embedded sections, proplatelet formation assay, and assay of colony-forming unit-megakaryocyte are detailed in the Online Supplementary Data.
Western blot analysis
Freshly isolated megakaryocytes or platelets were washed with phosphate-buffered saline, and the pellet was re-suspended in cell lysis buffer (Cell Signaling Technology) containing protease inhibitor (1:100 dilution, Cell Signaling Technology). Further details are given in the Online Supplementary Data.
Blood smears were fixed with methanol for 10 min at room temperature followed by Wright-Giemsa staining. For immunofluorescence microscopy, livers were obtained from mice after perfusion. OCT-embedded livers were cut into 8 m sections and stained with anti-F4/80 (Abcam) and anti-CD41 antibodies for Küpffer cells and platelets, respectively. After mounting, immunostaining was detected using an inverted confocal fluorescence microscope (TCSSP8, Leica). For transmission electron microscopy, bone marrow was fixed with 2.5% glutaraldehyde and embedded in Epon as described previously.27 Further details are provided in the Online Supplementary Data.
Platelet glycan analysis
Glycan structure was analyzed based on our published methods. 7 In brief, platelets isolated from wild-type (WT) or Plt Slc35a1–/– mice were dissolved in Ambic buffer (50 mM ammonium bicarbonate). Samples were heated for 5 min at 100°C for denaturation and then digested with trypsin (37°C, overnight). After tryptic digestion, the N-glycans were released using PNGase F (New England BioLabs) and the N-linked glycan fraction was eluted through a C18 reversed phase cartridge. Subsequently, O-glycans were released by β-elimination. Both N-glycans and O-glycans were permethylated and analyzed by matrix-assisted laser-desorption time-of-flight (MALDI-TOF) tandem mass spectrometry (MS/MS) (Online Supplementary Figure S3).
The unpaired Student t-test was used to determine P values as indicated in the figures.
Generation of mice with megakaryocyte- and platelet-specific deletion of Slc35a1
To generate the Slc35a1f/f mice, exons 3 and 4 of the Slc35a1 gene were flanked with loxP sites (Figure 1B). Floxed Slc35a1f/+ embryonic stem cells were injected into blastocysts of C57BL/6J mice to obtain chimeric mice. The derived mice were mated with C57BL/6J mice to achieve germline transmission of the floxed allele. Slc35a1f/+ female mice were then mated with Slc35a1f/+ male mice to generate Slc35a1f/fmice. Slc35a1f/fmice were crossed with Pf4Cre mice to generate Slc35a1f/f;Pf4Cre mice (Plt Slc35a1–/–). Mouse genotypes were confirmed by polymerase chain reaction (PCR) by using tail genomic DNA to amplify a 273-bp product and a 343-bp product from the WT allele and Slc35a1 floxed allele, respectively (Figure 1C). Slc35a1 expression in bone marrow megakaryocytes was abolished in Plt Slc35a1–/– mice as determined by quantitative reverse transcription PCR (Figure 1D).
To further confirm the Slc35a1 deletion in Slc35a1–/– platelets, we amplified the major coding exons 3 to 6 of Slc35a1 by reverse transcription PCR. We detected a PCR amplicon of the expected size in cDNA from WT but not from Slc35a1–/– platelets, indicating deletion of functional Slc35a1 transcripts in platelets from Plt Slc35a1–/– mice (Online Supplementary Figure S1).
Slc35a1–/– platelets and megakaryocytes are deficient in sialylation
The glycosylation profiles of WT and Slc35a1–/– platelets and megakaryocytes were analyzed by flow cytometry and confocal microscopy based on lectin staining. For the lectin-based flow cytometry, we used biotinylated lectins, which enabled us to use PE-streptavidin only as a negative control. Neuraminidase-treated samples were used as a positive control (Figure 2A, Online Supplementary Figure S2). Binding of SNA (specific for α2,6-sialic acid) was not observed on either WT or Slc35a1–/– platelets (data not shown). RCA 1 (specific for terminal β-galactose) binding was increased in Slc35a1–/– platelets compared to that in WT platelets (Figure 2A). In contrast, MAL II (specific for terminal α2,3-sialic acid) binding was statistically decreased in Slc35a1–/– platelets when compared to its biding in WT platelets (Figure 2A). To determine whether platelet size affected the lectin measurement, we costained lectins with anti-CD41 antibody and calculated ratios of their mean fluorescent intensity (MFI). The MFI of RCA 1/CD41 increased significantly while the MFI of MAL II/CD41 decreased significantly in Slc35a1–/– platelets compared to the intensities in WT platelets (Figure 2A). All Slc35a1–/– megakaryocytes were positive for RCA 1 staining by both flow cytometry and confocal microscopy analysis (Figure 2B and C). However, for unknown reasons, we were unsuccessful in acquiring reproducible MAL II staining results either by flow cytometric analysis or immunostaining of megakaryocytes. These results demonstrated that disruption of Slc35a1 results in a significant reduction of sialylation in Slc35a1–/– megakaryocytes and platelets.
To complement these lectin-based analyses, we released, purified, and permethylated N- and O-linked glycans from each platelet sample, then performed MALDI-TOF mass spectrometry to determine the platelet sialylation pattern, followed by nanospray ionization- MS/MS to confirm several structures by collision-induced dissociation (Figure 2D and E; Online Supplementary Figures S3 and S4). For N-glycosylation, there was a significant increase of un-sialylation (from 28.6% to 50.1%) in Slc35a1–/– platelets, whereas N-glycolylneuraminic acid (NeuGc), a major isoform of sialic acids in non-human mammals, exhibited a decreased trend (from 71.4% to 49.9%). Moreover, NeuGc isoform analysis showed that one of the NeuGc isoforms was undetectable in Slc35a1–/– platelets (Online Supplementary Figure S4A). Glycan structure analysis indicated that O-glycans such as sialylated (NeuGc) core 1 structures at m/z 669 decreased signific - antly in Slc35a–/– platelets (Online Supplementary Figure S4B, Online Supplementary Table S1).
We noticed that residual sialylation was detected by the high resolution MALDI-TOF-MS/MS analysis in Slc35a1–/– platelets. As Plt Slc35a1–/– mice have normal sialylation in cells including hematopoietic cells other than mega - kyocytes/platelets, we hypothesized that residual sialylation in Slc35a1–/– platelets is caused by exogenous plasma sialylated molecules, such as fibrinogen and IgG which are abundant in the plasma and known to be sialylated, internalized by circulating platelets.28-31 In addition, exogenous IgG is also commonly found on the surface of platelets (PAIgG).30,31 This can be reflected in the fact that the relative ratios of singly to doubly-sialylated glycoforms remained consistent to each other in the WT and Slc35a1– /– platelets, rather than the number of doubly-sialylated glycoforms decreasing in addition to the singly-sialylated and non-sialylated species increasing in incomplete Slc35a1–/– platelets. To test the hypothesis that exogenous sialylated plasma proteins contributed to the residual sialic acids in the Slc35a1–/– platelets, we immunoprecipitated IgG from platelet lysates using protein A/G beads, and then blotted the immunoprecipitated IgG with MALII lectin that binds to α2,3-sialic acids. Our results indicated that both WT and Slc35a1–/– platelets contained MALIIpositive IgG (Online Supplementary Figure S5). As megakaryocytes and platelets do not synthesize IgG, this result indicates that Slc35a1–/– platelets contain exogenous sialylated proteins, which supports our hypothesis. On the basis of all these findings, we concluded that Slc35a1– /– megakaryocytes and platelets have significantly reduced sialylation (Figure 2; Online Supplementary Figures S4 and S5; Online Supplementary Table S1), thus being an appropriate model to study the effect of sialylation on endogenous sialylated molecules in megakaryogenesis and platelet homeostasis.
To determine whether abnormal sialic acid accumulation existed or not, we checked free sialic acid levels in serum and found that the sialic acid levels were identical in serum from WT and Slc35a1–/– mice (Online Supplementary Figure S6).
Plt Slc35a1–/– mice exhibit macrothrombocytopenia
The peripheral platelet count of Plt Slc35a1–/– mice was significantly lower than that of WT mice, and the mean platelet volume of Slc35a1–/– platelets was significantly larger than that of WT platelets (Figure 3A and Online Supplementary Figure S7A). Giemsa staining of blood smears also confirmed fewer platelets in Plt Slc35a1–/–mice (Figure 3B). Tail bleeding time analysis showed no significant difference between WT and Plt Slc35a1–/–mice (Online Supplementary Figure S7B). Since thrombopoietin regulates megakaryopoiesis and platelet formation, we measured plasma thrombopoietin level and found that it was significantly increased in Plt Slc35a1–/– mice (Figure 3C). However, although the percentage of reticulated platelets was higher, the absolute count of reticulated platelets in Plt Slc35a1–/– mice was lower than that in WT mice (Figure 3D and E). These results suggest that platelet production was impaired in Plt Slc35a1–/– mice.
There was also no difference in leukocyte count and hemoglobin content in peripheral blood between WT and Plt Slc35a1–/– mice (Online Supplementary Figure S7C and D). Histology showed no abnormality of major organs (liver, spleen, heart and kidney) in Plt Slc35a1–/– mice (Online Supplementary Figure S8).
Slc35a1–/– platelets express lower levels of GPIbα
Platelet GPIbα (CD42b) and integrin αIIbβ3 (CD41, CD61) are heavily glycosylated. To examine whether Slc35a1 deficiency impaired the surface levels of platelet glycoproteins, we measured GPIbα and integrin αIIbβ3 on WT and Slc35a1–/– platelets by flow cytometry. Our results showed that the level of GPIbα was decreased on Slc35a1–/– platelets (Figure 4A). However, no difference in integrin αIIbβ3 level was observed between WT and Slc35a1–/– platelets (Figure 4A). Likewise, there was no significant differences in GPIbα and integrin αIIbβ3 levels between WT and Slc35a1–/– megakaryocytes (Figure 4B). Western blot analysis indicated reduced total GPIbα content in Slc35a1–/– platelets (Online Supplementary Figure S9), suggesting that GPIbα shedding, but not internalization, is likely the cause of the reduced level of GPIbα on Slc35a1–/– platelets.33,34
Deletion of Slc35a1 results in impaired megakaryocyte differentiation and maturation
Platelets are primarily produced by megakaryocytes in the bone marrow. Plt Slc35a1–/– mice exhibited severe thrombocytopenia, raising the question of whether platelet production was affected by the absence of Slc35a1. Hematoxylin & eosin-stained, paraffin-embedded sections of bone marrow from of WT and Plt Slc35a1–/– mice showed significantly decreased numbers of megakaryo - cytes (Figure 5A). A colony-forming unit-megakaryocyte assay was performed to determine the proliferation and differentiation of megakaryocytes, and we found that the proliferation potential of megakaryocytes from Plt Slc35a1– /– mice was decreased (Figure 5B). Giemsa-stained bone marrow smears indicated that megakaryocyte development and maturation were impaired in Plt Slc35a1–/– mice relative to these processes in the WT mice (Figure 5C). At the ultrastructural level, the WT megakaryocytes exhibited a distinct granular zone containing a well-developed demarcation membrane system (Figure 5D, top). In contrast, the demarcation membrane system of Plt Slc35a1–/– megakaryocytes was disorganized with fewer well-delimited platelet territories (Figure 5D, bottom).
To determine which developmental stages of megakaryo cytes are affected by the loss of Slc35a1, we used multicolor flow cytometry to analyze isolated bone marrow cells (Online Supplementary Figure S10). Flow cytometry detected no difference in the percentage of megakaryocyte/erythroid progenitors and megakaryocyte progenitors between WT and Plt Slc35a1–/– mice (Figure 6A).35,36 Megakaryocyte differentiation correlates with increased DNA content, so we examined DNA ploidy of bone marrow megakaryocytes from WT and Plt Slc35a1–/– mice by flow cytometry (Online Supplementary Figure S11). Ploidy analysis using Hoechst33342 staining on bone marrow cells showed a significantly higher percentage of megakaryocytes at the early 2N stage in Slc35a1–/– mice than in WT mice (Figure 6B). To determine whether thrombocytopenia in Plt Slc35a1–/– mice was caused by defects in the production of proplatelets from megakaryo - cytes, we performed a proplatelet formation assay. CD41+ megakaryocytes with cytoplasmic pseudopods longer than the diameter of cytoplasm were defined as proplatelet- forming megakaryocytes (Figure 6C). Fibrinogeninduced proplatelet formation identified by immunostaining with anti-β-tubulin and anti-CD41 antibodies showed that megakaryocytes in Plt Slc35a1–/– mice displayed a reduced percentage of proplatelet-forming megakaryocytes when compared to the percentage in WT mice (Figure 6C). In addition, proplatelets from Plt Slc35a1–/– megakaryocytes were shorter and less branched compared to those from WT megakaryocytes.
The interaction of thrombopoietin and its receptor Mpl is critical for platelet production. N-glycosylation has been reported to regulate Mpl stability and function.37 In our study, western blot analysis showed that Mpl was decreased in Slc35a1–/– bone marrow megakaryocytes (Figure 7A and B). Megakaryocyte-specific Mpl transcripts of WT and Plt Slc35a1–/– mice were analyzed by quantitative reverse transcription PCR, but no significant difference was detected, indicating that the reduction in Mpl may be due to decreased stability (Figure 7C).
Desialylated platelets of Plt Slc35a1–/– mice are cleared by Küpffer cells in the liver
Glycan modifications regulate platelet clearance.7,13 To confirm whether this mechanism contributes to thrombocytopenia in Plt Slc35a1–/– mice, we first analyzed liver and spleen by immunofluorescence staining. Spleen is the major organ for platelet clearance, but there was no difference in the numbers of platelets in spleens from WT and Plt Slc35a1–/– mice (data not shown). Our previous study indicated that Küpffer cells in the liver are critical for the clearance of desialylated platelets.7 We therefore performed confocal imaging analysis of liver sections from WT and Plt Slc35a1–/– mice after staining with anti-F4/80 and anti-CD41 antibodies. The results showed that CD41+ Slc35a1–/– platelets are primarily in contact with F4/80+ Küpffer cells (Figure 8A and B). These data indicate that desialylated Slc35a1–/– platelets are mainly cleared by Küpffer cells in the liver.
As a common terminal glycosyl group, sialic acid can be attached to non-reducing terminal galactose (Gal) residue via α2,3-, or α2,6-linkage, to GalNAc via α2,6-linkage, and to sialic acid via α2,8-linkage.8,9 The biosynthesis of these diversified forms of sialylation is controlled by more than 20 different sialyltransferases.11,19,38 Even though these enzymes are differentially expressed in different tissues to regulate the specific sialylation pattern of cells, many of them have overlapping functions. Thus, it is difficult to determine the overall biological role of sialylation in a given specific cell type. However, the CST, encoded by Slc35a1, is essential to transport CMP-SA from the cytosol into the Golgi apparatus for the sialylation process.39 Therefore, we generated conditional Slc35a1f/f mice for cell-specific deletion of sialylation. Sialylation is the major capping glycan structure on platelet membrane glycoproteins. Although significant progress has been made recently, 11 the biological function of total sialylation on megakaryocytes and platelets is not yet fully understood. Moreover, patients with sialylation defects, such as SLC35A1-CDG, exhibited macrothrombocytopenia in common.20-23,40 To investigate this issue, we generated a Plt Slc35a1–/– mouse model. Plt Slc35a1–/– mice exhibited impaired megakaryocytopoiesis, impaired megakaryocyte maturation, and excessive platelet clearance in the liver, indicating that sialylation is essential for both platelet generation and clearance.
Platelets express several sialyltransferases, such as ST3Gal-I and ST3Gal-IV.18,19,41 Macrothrombocytopenia is a major phenotype of Plt Slc35a1–/– mice, which is consistent with mice lacking ST3Gal-I or ST3Gal-IV.17,41,42 These data indicate that sialylation is critical for platelet homeo - stasis. Like mice lacking ST3Gal-IV, Plt Slc35a1–/– mice showed increased clearance of platelets in the liver. However, we did not detect significant numbers of desialylated platelets co-localized with hepatocytes as reported in ST3Gal-IV-deficient mice.17 Instead, we found that desialylated platelets were primarily co-localized with Küpffer cells in the liver. This result is consistent with our previous publication,7 indicating that increased clearance mediated by the Küpffer cells in the liver contributes to thrombocytopenia in Plt Slc35a1–/– mice. The increased clearance of desialylated platelets in the liver and reduced numbers of megakaryocytes in the bone marrow found in Plt Slc35a1–/– mice was not found in mice lacking ST3Gal- I, which have normal platelet clearance and normal megakaryocyte number, as reported in an abstract published in 2014.42 This discrepancy might be caused by redundant functions of different sialyltransferases in platelets. However, further studies comparing different mouse models directly are required to provide more insights into how sialylation regulates the homeostasis of megakaryocytes/platelets.
Thrombopoietin and its receptor Mpl are major regulators of megakaryocytopoiesis, megakaryocyte maturation, and platelet production.14,43 Our western blot analysis showed that Mpl expression was reduced in Slc35a1–/– megakaryocytes, and quantitative reverse transcription PCR revealed no difference in Mpl transcripts between WT and Plt Slc35a1–/– megakaryocytes, suggesting reduced Mpl expression in Slc35a1–/– megakaryocytesis is likely caused by impaired protein stability. Deficiency of Mpl may contribute to the defective platelet production as the percentage of proplatelet-forming megakaryocytes and absolute count of reticulated platelets were significantly decreased in Plt Slc35a1–/–mice, although we did not reproducibly detect impaired Mpl-mediated signaling in Slc35a1–/– megakaryocytes. Desialylation of other glycoproteins, such as GPIbα, on megakaryocytes and platelets may also contribute to reduced platelet production. Previous studies have shown that refrigeration-mediated desialylation in platelets results in GPIbα shedding in vitro.44,45 In our study, while both Slc35a1–/– megakaryocytes and platelets exhibited significant reductions of sialylation, GPIbα level was only decreased on Slc35a1–/– platelets, while it remained unchanged on Slc35a1–/– megakaryocytes. These results support that reduced sialylation causes shedding of GPIbα on circulating platelets, likely due to the action of plasma proteases. However, as the level of GPIbα on Slc35a1–/– megakaryocytes is unchanged, it less likely to contribute to reduced platelet generation. Nevertheless, whether desialylation directly affects the function of these surface glycoproteins needs further study.
Genetic mutations of the SLC35A1 gene cause a rare form of a congenital disorder of glycosylation, SLC35A1- CDG. So far, only three human cases associated with SLC35A1 mutations have been reported. A recent study of such a patient showed that CST is not required for proplatelet formation,23 a finding that differs from our mouse results which show that Slc35a1–/– megakaryocytes have defective proplatelet formation. There are a few differences that may contribute to some of the inconsistencies between the study of the human patient and our mouse model, including: (i) the SLC35A1-CDG patient has a point mutation in the transmembrane domain, which might still have some residual CMP-SA transporting activities; (ii) in vitro-differentiated megakaryocytes from isolated CD34+ cells from the patient’s peripheral blood were used in the human study while bone marrow megakaryocytes were used in our study; (iii) the SLC35A1-CDG patient has reduced sialylation in all cell types while our mouse model has deletion of Slc35a1 specifically in megakaryocytes and platelets, and (iv) potential differences between human and mouse megakaryocytes. Nevertheless, we believe our mouse model, which is the first mouse model of Slc35a1 deficiency in megakaryo - cytes and platelets, will be valuable for determining the role of sialylation in platelet biology and helping to dissect the molecular and cellular pathogenesis of thrombocytopenia, a primary clinical presentation of patients with SLC35A1-CDG. The study of patients and the mouse model are complementary.
In addition to SLC35A1-CDG, desialylation contributes to many acquired platelet disorders, such as refractory immune thrombocytopenia and prolonged isolated thrombocytopenia after hematopoietic stem cell transplantation. 45,46 Our current and future studies using the Plt Slc35a1–/– mice as a tool may reveal more insights into the pathogenesis of these platelet problems, which may in turn lead to new diagnoses and therapies.
- Received May 7, 2019
- Accepted March 19, 2020
This study is a collaboration between Dr. Changgeng Ruan's laboratory at Jiangsu Institute of Hematology (JIH), Soochow University, China, and the Oklahoma Medical Research Foundation (OMRF), USA. The study was funded by JIH and OMRF. There are no intellectual conflicts of interests.
XM, YL, YK, JH, SAA and PA designed and conducted experiments, analyzed data, and drafted the manuscript. HS, YK, YJ, and XB contributed to manuscript preparation. JF, CR and LX designed the study, analyzed data, and wrote the paper.
This study was supported by grants from the National Natural Science Foundation of China (81520108005,81470825, 30928010, 81370617, 81800114), Jiangsu Provincial Key Medical Center (ZX201102), Jiangsu Provincial Special Program of Medical Science (BL2012005), and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions (YXZXA2016002), National Institutes of Health (IS10OD018530, P41GM10349010), and a research grant from Oklahoma Medical Research Foundation.
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