Hypoferremia results as an acute phase response to infection and inflammation aiming to reduce iron availability to pathogens. Activation of toll-like receptors (TLRs), the key sensors of the innate immune system, induces hypoferremia mainly through the rise of the iron hormone hepcidin. Conversely, stimulation of erythropoiesis suppresses hepcidin expression via induction of the erythropoietin-responsive hormone erythroferrone. Iron deficiency stimulates transcription of the osteocyte-secreted protein FGF23. Here we hypothesized that induction of FGF23 in response to TLR4 activation is a potent contributor to hypoferremia and, thus, impairment of its activity may alleviate hypoferremia induced by lipopolysaccharide (LPS), a TLR 4 agonist. We used the C-terminal tail of FGF23 to impair endogenous full-length FGF23 signaling in wild-type mice, and investigated its impact on hypoferremia. Our data show that FGF23 is induced as early as pro-inflammatory cytokines in response to LPS, followed by upregulation of hepcidin and downregulation of erythropoietin (Epo) expression in addition to decreased serum iron and transferrin saturation. Further, LPS-induced hepatic and circulating hepcidin were significantly reduced by FGF23 signaling disruption. Accordingly, iron sequestration in liver and spleen caused by TLR4 activation was completely abrogated by FGF23 signaling inhibition, resulting in alleviation of serum iron and transferrin saturation deficit. Taken together, our studies highlight for the first time that inhibition of FGF23 signaling alleviates LPS-induced acute hypoferremia.
Hypoferremia develops as a response to innate immune system activation following inflammation or infection. Iron is an essential element required for the survival, proliferation, and virulence of pathogens. The hypoferremic response is an important defense mechanism that aims to prevent iron bioavailability to pathogens by sequestration of iron within macrophages, resulting in a significant reduction in circulating iron levels.1-3 Toll-like receptors (TLR) are key components of the innate immune system that recognize pathogen-associated molecular patterns (PAMP), and are responsible for sensing invading pathogens. Activation of TLR signaling plays a pivotal role in the development of the hypoferremic host response.2,3 Lipopolysaccharide (LPS), a PAMP that is the major component of the outer membrane of Gram-negative bacteria, is known to activate TLR4. Injection of LPS to mice or humans causes a rapid release of pro-inflammatory cytokines and triggers a well characterized acute phase inflammatory response followed by an induction of the hepatic hormone hepcidin, the central regulator of iron homeostasis.4-6
Hepcidin binds to ferroportin, the only known iron exporter, and causes its internalization and degradation,7 resulting in iron sequestration in reticulo-endothelial macrophages, hepatocytes, and duodenal enterocytes, and subsequently decreased circulating iron levels.8 This situation of circulating iron restriction promotes ineffective erythropoiesis and results in anemia of inflammation, a form of anemia commonly associated with infectious diseases, cancer, and chronic kidney disease (CKD).9
CKD patients have increased iron losses due to chronic bleeding from impaired intestinal iron absorption, uremiaassociated platelet dysfunction, frequent phlebotomy, and blood trapping in the dialysis apparatus.10 In addition, circulating levels of fibroblast growth factor 23 (FGF23) dramatically increase in CKD, reaching up to 2,000-fold above the normal range in advanced renal failure.11 Recent studies have associated iron deficiency with the increase in FGF23 levels.12,13
FGF23 has been identified as the gene responsible for the phosphate wasting disorder autosomal dominant hypophosphatemic rickets (ADHR)14 and as a causative factor of tumor-induced osteomalacia.15 FGF23 is a bonederived endocrine regulator of phosphate and vitamin D homeostasis and bone mineralization.16-20 FGF23 inhibits phosphate reabsorption in the renal proximal tubule by suppressing the expression of the type II sodium-phosphate transporters NaPi2a and NaPi2c in the brush border membrane.17,21-23 Furthermore, FGF23 reduces circulating levels of 1,25-dihydroxyvitamin D3 by suppressing the renal expression of 1α-hydroxylase, the enzyme required for production of 1,25-dihydroxyvitamin D3, and increasing the activity of 24-hydroxylase, the enzyme that metabolizes 1,25-dihydroxyvitamin D3.17 FGF23 requires a co-receptor, klotho, for FGF receptor (FGFR) activation, and its effects are mediated through multiple FGFR, with FGFR1 being the principal receptor to mediate the effects of FGF23 on phosphate regulation, and FGFR3 and 4 mediating the FGF23 effects on vitamin D metabolism.24 In the kidney, FGF23 binds to the FGFR-klotho complex and induces signaling through Ras/MAPK, PI3K/Akt, and PLCγ/PKC downstream pathways which regulate mineral metabolism genes.25 FGF23 activity is regulated post-translationally by proteolytic cleavage between Arg179 and Ser180, and O-linked glycosylation. Cleavage of the intact full-length FGF23 peptide by a subtilisin-like pro-protein convertase abolishes its biologic activity. Intact FGF23 contains an arginine–X–X–arginine (RXXR) motif which is recognized by pro-protein convertases such as furin. This motif is located at the boundary between the FGF core homology domain, which interacts with the FGFR, and the 72-residue-long C-terminal tail of FGF23, which interacts with the co-receptor Klotho.26-28
We recently reported that inhibition of FGF23 signaling rescues renal anemia in a mouse model of CKD.29 Here, we investigated the effect of inhibition of FGF23 signaling in a mouse model of LPS-induced hypoferremia. Our results show that FGF23 is induced in response to LPS prior to hepcidin upregulation followed by downregulation of Epo expression. Moreover, we show that inhibition of FGF23 signaling alleviates hypoferremia and attenuates dysregulation of erythropoiesis in a mouse model of acute inflammation induced by LPS.
C57BL/6J wild-type (WT) male mice between 8 and 10 weeks of age were purchased from The Jackson Laboratory (Bar Harbor, ME, USA) and housed at the New York University (NYU) College of Dentistry Animal Facility, where they were kept on a light/dark (12 hours [h]/12h) cycle at 23ºC, and received food (standard lab chow) containing 185 parts per million iron (Teklad 2018S; Harlan) and water ad libitum. All animal protocols were approved by the Institutional Animal Care and Use Committee (IACUC) at NYU. Mice were treated with a single intraperitoneal (i.p.) injection of 1 mg/kg of an FGF23 blocking peptide (provided by Dr. M. Mohammadi, NYU School of Medicine) or vehicle (HEPESbuffered saline; 25 mM HEPES-NaOH pH7.5, 150 mM NaCl). The FGF23 blocking peptide is the 72 aa long C-terminal tail of human FGF23 (residues Ser180-Ile251), which corresponds to the C-terminal fragment of FGF23 generated by proteolytic cleavage at the RXXR motif.27 Eight hours post treatment, acute inflammation was induced by a single i.p injection of 50 mg/kg of LPS (E. coli 055:B5, Sigma-Aldrich, St Louis, MO, USA). An equal volume of sterile saline solution (0.9% NaCl) was used as vehicle control. Mice were euthanized 4 h after LPS treatment. In longitudinal studies, saline- and LPS-treated mice were euthanized after 1, 2, 4, 6, 12, or 24 h of LPS treatment.
Blood, tissue, and serum collection
Mice were immediately necropsied after euthanasia and blood was collected by cardiac puncture. Complete blood count was performed using the VetScan HM5 Hematology Analyzer (ABAXIS, Union City, CA, USA). Serum was obtained after blood centrifugation at 3,500 rpm for 10 minutes (min). Liver, spleen, kidney, bones, and bone marrow cells obtained from femora and tibiae, were collected, snap-frozen in liquid nitrogen, and stored at -80°C until further use.
Serum FGF23 levels were measured using the mouse FGF23 Intact and C-terminal ELISA assays (Quidel Corporation/Immutopics International, San Clemente, CA, USA). The intact assay detects exclusively biologically intact FGF23 (iFGF23), whereas the C-terminus (cFGF23) assay is capable of detecting both the intact molecule and its C-terminal fragments. Serum phosphorus was determined by colorimetric measurements using the Stanbio Phosphorus Liqui-UV Test reagent (Stanbio Laboratory, Boerne, TX, USA). Serum iron and transferrin saturation were measured using the Iron-TIBC kit from Pointe Scientific (Canton, MI, USA). Serum hepcidin was measured using the Hepcidin Murine-Compete ELISA kit (Intrinsic LifeSciences, La Jolla, CA, USA). Serum EPO levels were measured using the Rat/Mouse EPO Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA).
Tissue iron measurement
RNA isolation, reverse transcription, and real-time quantitative polymerase chain reaction analysis
Total RNA was extracted from kidneys, liver, whole bone, spleen, and bone marrow using Trizol (Ambion; Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol (Molecular Research Center, Cincinnati, OH, USA). Synthesis of cDNA was performed using the High Capacity cDNA Reverse Transcription Kit as described by the manufacturer (Applied Biosystems; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was amplified by quantitative polymerase chain reaction (qPCR) using the PerfecCTa SYBR Green SuperMix (Quanta Biosciences, Gaithersburg, MD, USA). All primers used in this study are listed in the Online Supplementary Table S1. mRNA levels were normalized to the housekeeping gene (Gadph or Hprt) in the same cDNA sample. The relative transcript expression of a gene is given as DCt=Cttarget−Ctreference. The fold change in gene expression, as compared to control mice, was determined as 2−DDCt values (DDCt=DCttreated−DCtcontrol).
Snap frozen samples of spleen and liver were homogenized in an ice-cold RIPA buffer (Sigma-Aldrich, St. Louis, MO, USA) with addition of EDTA-free HaltTM Protease Inhibitor Single-use Cocktail and HaltTM Phosphatase Inhibitor Single-Use Cocktail (both from Thermo Scientific, Rockford, lL, USA) and were lysed for 2 h on ice with vortexing every 15 min. After lysis, samples were centrifuged for 20 min at 13,000 g and protein concentration was measured in supernatants by Quick Start Bradford Dye Reagent according to the manufacturer’s instructions. For each sample, 30 mg of protein was loaded into 4-20% Mini-Protean® TGX™ Precast Protein Gels. Electrophoresis was performed for 18 min on a constant voltage of 300 V in a Tris/Glycine/SDS buffer. Then proteins were transferred to 0.45 mm nitrocellulose membrane by wet method with Tris/Glycine Transfer buffer (Quality Biological Inc., Gaithersburg, MD, USA) with the addition of 20% methanol (Fisher Scientific, Hampton, NH, USA) for 1 h on a constant voltage of 110 V. Membranes were then washed with Trisbuffered saline Tween (TBST) (Fisher Scientific, Hampton, NH, USA) and blocked for 2 h at room temperature (RT) with either 5% non-fat dry milk (LabScientific Inc., Highlands, NJ, USA) in TBST (for anti-ferroportin and anti-STAT3 antibodies) or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in TBST (for anti-phospho- STAT3 antibodies) and washed four times with TBST. Membranes were stained with primary antibodies overnight at 4°C: anti-STAT3 mouse antibody (1:1,000 on 5% non-fat dry milk-TBST), anti-phospho-STAT3 (Tyr705) rabbit antibody (1:2,000 on 5% BSA-TBST) (#9139 and #9145, respectively, Cell Signaling Technology, Danvers, MA, USA), anti-ferroportin rabbit antibody (1:1,000 on 5% non-fat dry milk TBST) (#MTP11-S, Alpha Diagnostic Intl Inc., San Antonio, TX, USA), and anti-Actin goat antibody (1:1,000 on TBST) (sc-1616, Santa Cruz Biotechnology Inc., Dallas, TX, USA). Membranes were stained with secondary antibodies diluted 1:10,000 on TBST for 30 min at RT: mouse-anti-rabbit IgG-HRP, goat-anti-mouse IgG-HRP and Rb-anti-Gt IgG-HRP (all from Santa Cruz Biotechnology Inc., Dallas, TX, USA). After incubation, membranes were washed with TBST four times. Visualization was performed with Clarity Western ECL Substrate and ChemiDocTM XRS+ imager, images were analyzed with Image LabTM, and protein signal intensity was calculated with ImageJ (NIH, Bethesda, MD, USA). All reagents, materials, devices and software were purchased from Bio-Rad Laboratories Inc., Hercules, CA, USA, unless otherwise specified.
All data were analyzed for normal distribution by Shapiro-Wilk tests and “Normal Q-Q Plot” graphs. If normal distribution was not achieved, data were aligned in rank transformation and a normality test was performed. The equality of variance was determined with Levene’s test. If both normal distribution and equality of variance were demonstrated, data were analyzed by two-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test. When data were not normally distributed, non-parametric test was performed by Kruskal-Wallis test using SigmaStat software (SPSS Sciences, Chicago, IL, USA) and GraphPad Prism 8 (2019 GraphPad Software, Inc. La Jolla, CA, USA). All values are presented as mean+standard deviation (SD). P<0.05 was considered statistically significant.
Early induction of Fgf23 and regulation of phosphate homeostasis in response to lipopolysaccharide
To identify the series of events that take place in response to inflammation, we carried out longitudinal studies during which we injected a sub-lethal dose (50 mg/kg) of LPS to wild-type mice and evaluated its effect on inflammatory markers, FGF23 levels, and phosphate homeostasis after 1, 2, 4, 6, 12, and 24 h. As expected, liver pro-inflammatory cytokines such as IL-6, TNF-a, and IL-1b were significantly induced during the first 6 h of LPS treatment, reaching a peak at 1 h, and returning progressively to baseline levels by 12 h upon resolution of inflammation (Figure 1A-C). Furthermore, we investigated the effect of LPS treatment on Fgf23 expression and circulating FGF23 levels. Both serum intact and Cterminal FGF23 levels were significantly elevated between 2-6 h or 2-12 h, respectively, in response to LPS (Figure 1D and E). Bone Fgf23 expression increased 4 h after LPS treatment (Online Supplementary Figure S1A). However, in liver, Fgf23 expression was significantly upregulated 2 h after LPS treatment and remained elevated up to 12 h, before progressively returning to basal levels by 24 h (Figure 1F). In addition, Fgf23 expression was markedly increased in spleen and bone marrow during the first 12 h after LPS treatment and returned to basal levels by 24 h (Figure 1G and Online Supplementary Figure S1B). Serum phosphate levels were significantly increased the first 2 h of LPS treatment, and they were associated with a concurrent significant decrease in urinary phosphate excretion (Figure 2A), possibly causing the induction of FGF23. In a counter-regulatory manner, the increase in circulating FGF23 led to a significant increase in urinary phosphate levels 6 h after LPS treatment, resulting in normalization of serum phosphate levels (Figure 2A). In kidney, FGF23 binds to the FGFRKlotho complex to induce phosphate excretion by downregulating the sodium-phosphate transporters NaPi2a/c. Consistent with increased FGF23 levels and urinary phosphate excretion, renal expression of NaPi2a, NaPi2c, as well as Klotho, decreased significantly during the period of FGF23 elevation in response to LPS, before returning to basal levels by 24 h (Figure 2B-D). Taken together, our data demonstrate that, in response to LPS, FGF23 is induced as early as inflammatory markers, resulting in regulation of phosphate homeostasis by promoting phosphate excretion.
Acute inflammation induces hypoferremia and downregulates Epo expression
Inflammation is a major stimulus for hepcidin synthesis in the liver. Specifically, treatment of hepatocytes with LPS leads to increase in hepcidin levels.32,33 In agreement with published studies, our data show that hepcidin expression started rising 1 h after LPS treatment with significant upregulation between 4-6 h after LPS (Figure 3A), consistent with the presence of inflammation (Figure 1A-C), and returned to basal levels after resolution of inflammation (Figure 3A). The increase in hepcidin resulted in progressive and significant reduction in circulating iron and transferrin saturation levels (Figure 3B and C), followed by recovery from hypoferremia once inflammation was resolved and hepcidin levels returned to normal. This drop in serum iron levels was accompanied by a significant decrease in ferroportin expression in liver and spleen (Figure 3D and E), leading to iron retention in these organs (Figure 3F and Online Supplementary Figure S2) and increased spleen weight (Figure 3G). In addition, expression of the acute phase protein lipocalin 2 (Lcn2), which plays a role as an iron sequester,34 was increased after 4 h of LPS and remained elevated up to 24 h post LPS treatment (Figure 3H). These data demonstrate that LPS induces hypoferremia associated with downregulation of ferroportin and iron retention in liver and spleen.
Studies have shown that pro-inflammatory cytokines, such as IL-6, TNF-a, and IL-1b, increased by inflammation and/or infection, inhibit renal erythropoietin (Epo) production, the primary regulator of erythropoiesis, and therefore suppress erythropoiesis.35-37 This is known as anemia of inflammation. Specifically, it has been shown that administration of LPS in rats significantly suppressed renal Epo gene expression.36 In our studies, we found that renal Epo expression was significantly downregulated after 4-6 h (Figure 3I).
Inhibition of FGF23 signaling alleviates hypoferremia induced by acute inflammation
It has been reported that the C-terminal tail of FGF23 mediates the binding of FGF23 to the FGFR-Klotho complex. 27,38 Studies have shown that the 72-aa-long C-terminal tail of FGF23 (residues Ser180-Ile251), which corresponds to the C-terminal fragment of FGF23 generated by proteolytic cleavage at the RXXR motif, impairs FGF23 signaling and activity by acting as endogenous inhibitor of the full-length FGF23 for binding to the FGFR-Klotho complex.27 We previously reported that inhibition of FGF23 signaling using this FGF23 blocking peptide (BL) ameliorates iron deficiency in a mouse model of CKD.29 Since FGF23 significantly increases in response to LPS, here we assessed the effect of inhibiting FGF23 signaling in a mouse model of hypoferremia induced by acute inflammation. Based on the results of our longitudinal studies, we opted to assess the effect of FGF23 signaling inhibition 4 h post LPS treatment. At this time point, we observed maximum induction of FGF23 and hepcidin, associated with a significant decrease in serum iron, transferrin saturation, and erythropoietin, during upregulation of pro-inflammatory cytokines.
Our data show that the increase in FGF23 in response to LPS was significantly reduced after inhibition of FGF23 signaling (Figure 4A-C). Administration of LPS triggered an acute inflammatory response (Figure 5A-C) that led to induction of hepcidin (Figure 5D and E). However, blocking FGF23 signaling significantly decreased hepatic expression of TNF-a without affecting IL-6 and IL-1b mRNA expression in LPS-treated mice (Figure 5A-C). Importantly, the increase in hepatic and circulating hepcidin levels induced by LPS was significantly reduced when FGF23 signaling was impaired (Figure 5D and E). We also found that inhibition of FGF23 signaling did not impact phosphorylation of STAT3, the main intracellular regulator of hepcidin induction by IL6, as treatment with cFGF23 did not affect LPS-induced upregulation of hepatic STAT3 phosphorylation (Online Supplementary Figure S3). Therefore, suppression of hepcidin by cFGF23 appears to be independent of the IL6/STAT3 pathway. These data suggest that inhibiting FGF23 signaling reduces LPS-induced hepcidin in mice with normal kidney function.
Increased hepcidin leads to iron sequestration in tissues and subsequent decrease in circulating iron levels. 7,8,39 As expected, LPS elicited hypoferremia after 4 h with a significant decrease in serum iron and transferrin saturation (Figure 6A and B), associated with a robust reduction in liver and spleen ferroportin (Fpn) mRNA (Figure 6C and D). Downregulation of ferroportin resulted in increased hepatic and splenic iron content (Figure 6E and F), which correlated with increased hepatic expression of the iron storage protein ferritin H (Fth) (Figure 6G) and the iron sequester lipocalin 2 (Lcn2) (Figure 6H). The number of circulating neutrophils, which are the main source of lipocalin secretion, was also increased in response to LPS (Table 1). Importantly, inhibition of FGF23 signaling increased serum iron and transferrin saturation levels in both basal and LPS conditions (Figure 6A and B), and prevented LPS-induced liver and spleen iron sequestration (Figure 6E and F). Circulating neutrophils and expression of ferritin H and lipocalin 2 in the liver were also significantly reduced after administration of the FGF23 C-tail blocking peptide in LPS-treated mice (Table 1 and Figure 6G and H). However, blocking FGF23 signaling did not change the expression of liver and spleen ferroportin in response to LPS (Figure 6C and D), but in basal conditions it selectively induced splenic ferroportin mRNA and protein expression compared to controls (Figure 6D and Online Supplementary Figure S4). Taken together, these data suggest that inhibition of FGF23 signaling can alleviate hypoferremia induced by acute inflammation in mice in the absence of renal disease.
Inhibition of FGF23 signaling increases renal and EPO and EpoR
Consistent with previously published data,36 our studies show that LPS significantly downregulated renal Epo and EpoR expression after 4 h (Figure 7A and B). We also found that renal Hif2α mRNA was significantly decreased after LPS treatment (Figure 7C), in line with the role of HIF2 as a regulator of Epo synthesis and iron metabolism.40 Importantly, inhibition of FGF23 signaling prevented the downregulation of renal Epo, EpoR, and Hif2α expression caused by LPS (Figure 7A-C). We previously reported that treatment with the FGF23 C-tail blocking peptide significantly increases circulating Epo levels in mice.29 Here, we confirmed this effect in mice injected with LPS or saline (Figure 7D) using a 10 times lower concentration of the FGF23 C-tail blocking peptide than the one we used in our previous work.29 However, we found that 4 h of LPS treatment at the concentration of 50 mg/kg were not sufficient to alter circulating Epo levels (Figure 7D). In addition to its effect on renal Epo and EpoR, LPS significantly decreased the expression of splenic and hepatic Epo and EpoR (Figure 7E-H), which was rescued by treatment with the FGF23 C-tail blocking peptide (Figure 7E-H). These data clearly demonstrate the efficiency of the C-tail FGF23 in increasing renal and extra-renal Epo and EpoR expression in the presence of inflammation, leading to increased serum Epo levels. Our findings also indicate that in the absence of inflammation, the C-tail FGF23 increases circulating Epo levels by upregulating splenic Epo expression without affecting renal or hepatic Epo expression.
Iron is an essential micronutrient for host-pathogen interaction. During infection, pathogens develop multiple strategies to acquire iron for proliferation and virulence, such as secretion of ferric iron-binding molecules known as siderophores.41 A host defense mechanism to inhibit pathogen growth is to deprive invading pathogens of iron, a process known as nutritional immunity by sequestering iron in tissues, thus limiting availability of circulating iron and resulting in hypoferremia.42 Iron homeostasis is controlled by two proteins, the hepatic hormone hepcidin which regulates iron absorption, and the iron exporter ferroportin which exports iron into the circulation from duodenal enterocytes, hepatocytes, and splenic reticuloendothelial macrophages that recycle the iron of senescent erythrocytes.43 Toll-like receptors (TLR) are key components of the innate immune system that recognize pathogen-associated molecular patterns (PAMP). Activation of TLR signaling plays a pivotal role in the development of the hypoferremic host response.2,3,44 Several toll like receptors such as TLR3, 4, 7, 7/8 and 9 have been described to induce hepcidin expression in vitro and in vivo.44
Several studies have demonstrated that iron deficiency induces FGF23 production.12,13,45 We recently reported that inhibition of FGF23 signaling using the C-tail FGF23 rescues anemia and iron deficiency in a CKD mouse model characterized by chronic inflammation, by increasing EPO, serum iron and ferritin levels, and attenuating inflammation.29 Our goals in the present study were: (i) to decipher the kinetics of Fgf23 in response to LPS in comparison to the kinetics of inflammatory, iron metabolism, and erythropoiesis parameters; and (ii) to investigate the impact of FGF23 signaling inhibition during LPS-induced acute inflammation.
Our study showed that FGF23 is induced as early as proinflammatory cytokines in response to LPS (Figure 1), followed by upregulation of hepcidin and decrease in serum iron and transferrin saturation (Figure 3A-C), suggesting that the early increase in FGF23 by LPS may contribute to hypoferremia. Previous studies have shown that FGF23 is produced by macrophages and acts locally to stimulate TNF-α expression via activation of FGFR1/α-Klotho signaling. 46 Moreover, acute inflammation induced by interleukin- 1β (IL-1β) or heat-killed Brucella abortus in mice significantly increased Fgf23 production.12 Thus, since FGF23 and inflammation positively regulate each other, it is possible that one condition may precede the other.
FGF23 is a hormone primarily produced by osteocytes that affects many systems in the body. FGF23 is crucial for maintaining normal phosphate and vitamin D homeostasis. Its target organ for these actions is the kidney, where it inhibits phosphate reabsorption by downregulating the type II sodium-phosphate co-transporters (NaPi2a and NaPi2c), and reduces circulating calcitriol levels (1,25(OH)2 vitamin D3) by decreasing renal expression of 1-α-hydroxylase. 17-20,47-50 FGF23 levels increase significantly in CKD and are associated with kidney disease progression, greater cardiovascular risk, left ventricular hypertrophy, and mortality. 25,51 Phosphate, calcium, vitamin D, and parathyroid hormone (PTH) are known regulators of FGF23. However, in recent years, several other factors, such as inflammation, iron status, and EPO have been identified to both regulate FGF23 and be regulated by it.13,29,52-58 Several mechanisms have been proposed to explain the association between FGF23 and inflammation. It has been shown that inflammation stimulates FGF23 either indirectly through increased hepcidin production, causing iron sequestration in macrophages and subsequent functional iron deficiency, or directly by stimulating Hif1α transcription leading to increased HIF1α which together with HIF1β bind to HIF response element on the Fgf23 promoter inducing its transcription.12 More recently, IL6 was reported to induce Fgf23 promoter activity through a STAT3 response element on the Fgf23 promoter,59 and a distal enhancer upstream of the Fgf23 transcription start site was shown to mediate inflammation-induced expression of Fgf23.60
FGF23 can directly target a number of different cell types and affect a variety of organs. Depending on the target, the mechanisms of FGF23 actions can differ in their requirement for klotho and specific FGFR isoforms, thereby activating different signaling pathways and leading to cell type- and tissue-specific effects of FGF23.61 For example, FGF23 can directly stimulate secretion of proinflammatory cytokines through activation of FGFR4 and PLC /calcineurin/NFAT signaling in the absence of klotho in the liver and bronchial epithelial cells in patients with CKD and chronic obstructive pulmonary disease (COPD), respectively.58,62 The lack of hepatic FGFR4 signaling investigation is a limitation of our study. Therefore, further experiments are required to delineate the hepatic FGFR signaling that contributes to hypoferremia. Mice deficient in Fgf23 (Fgf23-/-) exhibit aberrant bone mineralization along with high serum phosphate, calcium, and vitamin D levels, tissue and vascular calcifications, early lethality within 12 weeks after birth,19 in addition to inflammation and dysregulation of iron homeostasis (data not shown), thus limiting the possibility of using these mice to investigate the impact of FGF23 in response to hypoferremia. Here we evaluated the effect of pharmacologic inhibition of FGF23 signaling using the C-tail FGF23 in a mouse model of LPS-induced hypoferremia. Binding and cell signaling studies have shown that the C-tail of FGF23 can inhibit FGF23 signaling and antagonize its phosphaturic activity in vivo by competing with the intact FGF23 for binding to the FGFR-Klotho complex.27 Moreover, we have previously successfully used the C-tail FGF23 in a CKD mouse model to rescue renal anemia and iron deficiency and attenuate chronic inflammation.29
CKD patients, as well as mice with 5/6 nephrectomy, exhibit elevation in FGF23 levels associated with downregulation of renal Klotho expression. Our longitudinal studies showed that serum phosphate levels significantly increased the first 2 h of LPS treatment (Figure 2A), consistent with previous studies.63 Although the mechanism of this increase is not fully understood, studies have shown that LPS and TNF-α stimulate osteoclastogenesis and bone resorption, suggesting that, in conditions of systemic inflammation, there is a transcellular shift of phosphate from bone cells into the extracellular compartment, leading to hyperphosphatemia.63,64 In response to high serum phosphate levels, circulating FGF23 and Fgf23 expression significantly increased (Figure 1D-G), followed by appropriate decrease in renal Klotho and NaPi2a/c expression (Figure 2B-D) to increase urinary phosphate excretion (Figure 2A) in order to achieve normophosphatemia. We also investigated any potential impact of PTH on phosphate homeostasis and we found that PTH levels are 2.5- fold increased 4 h after LPS treatment and returned to basal levels by 6 h after treatment in comparison to vehicle- treated mice (0 h) (data not shown), suggesting the possibility that the rise in serum Pi 1-2 h after LPS may contribute to PTH induction, which in turn would induce phosphaturia. However, our data exclude the possibility that PTH contributes to the increase in FGF23 because the latter reaches peak levels in circulation at the same time point as PTH. Moreover, since vitamin D is a potent stimulator of FGF23, we measured the expression of Cyp27b1, the enzyme responsible for the synthesis of the bioactive form of vitamin D, in the kidney. We observed a 10-fold increase in renal Cyp27b1 expression 2 h after LPS treatment (data not shown), suggesting that circulating levels of vitamin D would be increased. However, FGF23 levels are already significantly elevated by 2 h after LPS, also excluding the possibility that vitamin D contributes to the increase in FGF23. The present study cannot determine whether the increase in FGF23 levels in response to LPS is due to the rise of inflammatory cytokines such as IL-6, TNF-α and IL-1β, the decrease in serum iron, and/or the increase in serum phosphate, all occurring after LPS treatment and prior to the increase in FGF23 levels.
In this study we also show that, although the main source of circulating FGF23 is normally the bone, in LPSinduced inflammation, organs involved in the immune system, such as the liver and spleen, significantly contribute to the increased circulating FGF23 levels (Figure 1D-G). In addition, blocking FGF23 signaling in LPS conditions resulted in significant reduction in bone (Figure 4A) and bone marrow (data not shown) Fgf23 mRNA levels, resulting in a modest but significant decrease in serum FGF23 levels (Figure 4B and C). However, spleen and liver Fgf23 expression were not affected by the cFGF23 in LPStreated animals (data not shown), suggesting that these two tissues contribute to the residual induction of circulating FGF23. This is in agreement with published data showing that spleen significantly contributes to elevated circulating FGF23 levels that rise in response to acute or chronic exposure to LPS.65,66 Moreover, mice with genetic deletion of Fgf23 (Fgf23-/-) or its co-receptor Klotho (Klotho-/-) exhibit a reduced number of splenocytes and thymic atrophy,49,67 suggesting that Fgf23 may play a role in the regulation of the innate and/or acquired immune system. Bone marrow Fgf23 was also significantly increased between 1-12 h after LPS treatment, possibly contributing also to the increased circulating FGF23 levels (Online Supplementary Figure S1B). Fgf23 production in bone was only significantly increased after 4 h of LPS; however, this increase correlates with a similar increase in bone Fgf23 in the saline-injected group at the same time point, suggesting that it may not be LPSdependent (Online Supplementary Figure S1A).
Moreover, in line with previously acquired knowledge that LPS-induced hypoferremia is caused through hepcidin- dependent and -independent mechanisms,68,69 our results show that treatment of mice with LPS significantly downregulates renal Epo expression (Figure 3I) following upregulation of pro-inflammatory cytokines and Fgf23 secretion. This is consistent with studies showing that inflammatory cytokines impair erythropoiesis by inhibiting production of Epo, and by directly inhibiting erythroid progenitor cell proliferation and differentiation.35-37,69
The present study is the first to highlight the effect of FGF23 signaling on iron metabolism and its contribution to hypoferremia. Here, we show that at steady-state conditions, disruption of FGF23 signaling results in increased circulating iron levels (Figure 6A) without affecting hepcidin (Figure 5D and E) or inflammatory status (Figure 5AC). This result is likely due to the rise of Epo induced by the C-tail FGF23 (Figure 7D) resulting in increased splenic Fpn mRNA and protein levels (Figure 7D and Online Supplementary Figure S4). Moreover, in response to LPS, mice treated with the C-tail FGF23 resist downregulation of Hif2α, a potent inducer of Epo, resulting in increased circulating EPO and Epo expression (Figure 7). Indeed, it has been reported that Epo directly stimulates intestinal iron absorption by increasing both iron uptake through upregulation of the divalent metal transporter 1 (Dmt1) and iron efflux through upregulation of the Fpn transporter. 70 Studies have also shown that HIF2α maintains iron balance by regulating transcription of Dmt1 and Fpn.71,72 Fpn is rapidly induced under low iron conditions via a HIF2α-dependent mechanism and disruption of HIF2α in the intestine inhibits the adaptive increase of intestinal Fpn during iron deficiency in mice.72 Together, the results of these studies suggest that HIF2α activation may induce iron mobilization, whereas inhibition of HIF2α will favor decreased iron absorption.71,72 A limitation of our study is the lack of data on the effect of cFGF23 on intestinal HIF2a, DMT1, and FPN protein levels, which could have provided evidence for a possible mechanism for the effect of cFGF23 on iron absorption during hypoferremia.
Our study also demonstrates that disruption of FGF23 signaling prevents LPS-induced hypoferremia by decreasing hepcidin (Figure 5D and E) and abrogating iron sequestration in liver and spleen (Figure 6). Suppression of hepcidin by cFGF23 in LPS-treated mice appears to be independent of the IL6/STAT3 pathway (Online Supplementary Figure S3). However, cFGF23 significantly decreases induction of Bmp6 transcription by LPS (Online Supplementary Figure S5), suggesting that cFGF23 may be affecting the BMP6/SMAD pathway, an important transcriptional pathway for hepcidin regulation. In support of this, our data show that splenic expression of erythroferrone (Erfe), a negative regulator of hepcidin, is decreased in mice treated with LPS, whereas mice treated with the cFGF23 under LPS conditions have significantly higher Erfe expression (Online Supplementary Figure S6). Erfe has been shown to suppress BMP/SMAD signaling in vivo and in vitro, and to inhibit hepcidin induction by BMP6.73 Thus, our data suggest that cFGF23 decreases hepcidin induction by LPS by increasing Erfe, and possibly suppressing the BMP6/SMAD pathway.
We recently described that inhibition of FGF23 signaling using 10 mg/kg of the C-tail FGF23 rescues anemia and iron deficiency in a CKD mouse model by stimulating erythropoiesis, increasing serum iron and ferritin levels, and attenuating chronic inflammation.29 In this experimental condition, where we observed highly elevated FGF23 levels and absolute iron deficiency characterized by a decrease in circulating iron29 and iron stores (Online Supplementary Figure S7), the C-tail FGF23 significantly reduced circulating FGF23 levels and improved the impaired iron distribution by elevating circulating iron and ferritin levels29 and increasing liver iron content (Online Supplementary Figure S7). In the present study, we show that even by decreasing the concentration of the C-tail FGF23 to 1 mg/kg, Epo is still induced at the steady state and after LPS (Figure 7D), and this concentration of the C-tail FGF23 is also sufficient to alleviate LPS-induced functional iron deficiency (Figure 6). However, this concentration is not sufficient to attenuate hepatic IL-6 and IL-1ɑ induction (Figure 5A and C), but it did significantly decrease TNF-a expression (Figure 5B). This is consistent with other studies showing that recombinant FGF23 and increased production of endogenous FGF23, such as in Hyp mice, stimulate TNF-a expression in macrophages.46 Thus, inhibition of FGF23 signaling represents a therapeutic target against absolute and functional iron deficiency pathologies.
Taken together, our results show for the first time that FGF23 signaling inhibition alleviates LPS-induced acute hypoferremia.
- Received September 2, 2019
- Accepted March 13, 2020
No conflicts of interests to disclose.
RA developed and directed the project, performed research and data analysis, and wrote the manuscript; MYP and CLH performed research, data and statistical analyses; SJ and AG performed research; GC purified and crystallized the C-tail FGF23 and analyzed its crystal structure; MM designed, refined, analyzed, and interpreted the crystal structure of the C-tail FGF23; DS developed and directed the project, performed data analysis, supervised the study, and wrote the manuscript.
The authors would like to thank Anna Montagna and Elena Healy for technical assistance.
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