Measurable residual disease monitoring provides insufficient lead-time to prevent morphologic relapse in the majority of patients with core-binding factor acute myeloid leukemia

Abstract

Core-binding factor acute myeloid leukemia is characterized by t(8;21) or inv(16) and the fusion proteins RUNX1-RUNX1T1 and CBFB-MYH11. International guidelines recommend monitoring for measurable residual disease every 3 months for 2 years after treatment. However, it is unknown if serial molecular monitoring can predict and prevent morphologic relapse. We conducted a retrospective single-center study of 114 patients in complete remission who underwent molecular monitoring with RT-qPCR of RUNX1-RUNX1T1 or CBFB-MYH11 transcripts every 3 months. Morphologic relapse was defined as re-emergence of >5% blasts and molecular relapse as ≥1 log increase in transcript level between 2 samples. Over a median follow-up time of 3.7 years (range 0.2-14.3), remission persisted in 71 (62.3%) patients but 43 (37.7%) developed molecular or morphologic relapse. Patients who achieved <3 log reduction in RUNX1-RUNX1T1 or CBFB-MYH11 transcripts at end of chemotherapy had a significantly higher risk of relapse compared to patients who achieved ≥3 log reduction (61.1% vs. 33.7%, p=0.004). The majority of relapses (74.4%, n=32) were not predicted by molecular monitoring and occurred rapidly with <100 days from molecular to morphologic relapse. Molecular monitoring enabled the detection of impending relapse and permitted pre-emptive intervention prior to morphologic relapse in only 11 (25.6%) patients. The current practice of molecular monitoring every 3 months provided insufficient lead-time to identify molecular relapses and prevent morphologic relapse in the majority of patients with core-binding factor acute myeloid leukemia treated at our institution. Further research is necessary to determine the optimal monitoring strategies for these patients.

Introduction

Core-binding factor (CBF) acute myeloid leukemia (AML) is recognized by the World Health Organization as a subtype of AML with recurrent genetic abnormalities characterized by the chromosomal abnormalities t(8;21) or inv(16)/t(16;16).¹ At the molecular level, these chromosomal alterations result in production of the fusion proteins RUNX1-RUNX1T1 in cases with t(8;21) and CBFB-MYH11 in those with inv(16)/t(16;16). CBF-AML accounts for 10-15% of adult AML and tends to be associated with younger patient age and higher sensitivity to induction and consolidation chemotherapy.^2,3 Despite a relatively favorable prognosis, up to 40% of patients with CBF-AML relapse.^2,4,5

The presence of minimal or measurable residual disease (MRD), defined as the presence of leukemic cells after treatment at levels of 1:10⁴ to 1:10⁶, predicts poor outcomes and impending relapse in AML.^6-8 Molecular monitoring for MRD is well-established in hematologic malignancies with clearly defined disease markers, such as BCR-ABL1 in chronic myeloid leukemia and NPM1 in NPM1-mutated AML. With the ability of real-time quantitative polymerase chain reaction (RT-qPCR) to quantify transcript levels of RUNX1-RUNX1T1 and CBFB-MYH11, CBF-AML represents one of the other most well-established targets for MRD monitoring in AML.⁸

Multiple studies have shown that the results of MRD monitoring can predict relapse in CBF-AML.^4,9-18 Given this capacity to identify patients at risk of imminent relapse, the recently published consensus statement from the European LeukemiaNet recommends molecular MRD monitoring for CBF-AML in the peripheral blood (PB) and bone marrow (BM) at diagnosis, after two cycles of induction/ consolidation chemotherapy, at the end of treatment, and every 3 months for 24 months of follow-up.⁶ Presumably, by monitoring patients for rising molecular transcripts, those at risk of impending relapse can be identified and treated with allogeneic bone marrow transplantation (BMT) prior to the emergence of overt disease and the need for re-induction chemotherapy. However, the relapse kinetics of CBF-AML are poorly understood, and it is not known whether serial MRD monitoring can detect molecular relapses with sufficient lead-time to intervene and prevent morphological relapse. Furthermore, it is uncertain if patients’ outcomes can be improved by using MRD status to risk-stratify patients and guide treatment decisions such as escalation to allogeneic BMT. We therefore conducted a study to determine the relapse kinetics and clinical utility of serial MRD monitoring in CBF-AML

Methods

Study design and population

This retrospective study included all patients ≥18 years old with CBF-AML at Princess Margaret Cancer Centre in Toronto, Canada between January 2000 and 2017. Patients were excluded if they did not have MRD monitoring performed. This study was approved by the institutional Research Ethics Board.

Treatment and measurable residual disease monitoring protocol

The standard treatment protocol for eligible patients with CBFAML at our institution during the study period was induction chemotherapy with daunorubicin for 3 days and continuous infusion of cytarabine for 7 days. Patients who achieved complete remission received three cycles of consolidation with high-dose cytarabine along with daunorubicin during the first cycle of consolidation (see Online Supplementary Methods). Allogeneic BMT was not routinely recommended unless there was molecular progression during follow-up or if risk factors were present such as t(8;21) with C-KIT mutation.¹⁹

Patients underwent MRD monitoring using RT-qPCR of RUNX1-RUNX1T1 or CBFB-MYH11 fusion transcripts in an accredited (ISO15189) laboratory at the University Health Network (see Online Supplementary Methods). Each patient’s own diagnostic level was used as baseline for calculating log reduction. The detection limit for these assays was 1 in 10,000 throughout the study period.

MRD measurements were generally performed at diagnosis, after induction and the final cycle of consolidation, and then every 3 months during follow-up for 24 months. MRD assessment was routinely performed on BM aspirates, but PB was tested in patients who could not tolerate repeat BM aspirates. A molecular relapse was typically confirmed with a repeat sample 4 weeks later, with referral for allogeneic BMT or administration of chemotherapy if confirmed. Patients who developed morphological relapse were treated with re-induction chemotherapy to achieve second complete remission and referred for allogeneic BMT if a suitable donor was available.¹⁹

Study protocol

We determined the risk of relapse among patients with CBFAML and the median time from molecular relapse to morphological relapse. We empirically defined rapid relapse as <100 days from molecular to morphological relapse as we considered this timeframe insufficient to directly administer allogeneic BMT to prevent morphological relapse. Morphological complete remission was defined as <5% blasts in BM with recovery of peripheral cell counts and no evidence of extramedullary disease. Morphological relapse was defined as the re-emergence of leukemic blasts in PB, ≥5% blasts in BM, or development of extramedullary disease.²⁰ Complete molecular remission was defined as two successive MRD-negative samples >4 weeks apart, and molecular relapse as an increase of MRD transcript level ≥1 log between two successive samples.⁶

Statistical analysis

We performed Student t-tests for continuous variables and a c² test/Fisher exact test for categorical variables to analyze differences between patients with remission or relapse and between those with rapid or slow relapse kinetics. A log-rank test was used to compare relapse-free survival between patients who achieved ≥3 or <3 log reduction in MRD at the end of treatment. Results with a P value <0.05 were considered statistically significant. All analyses were performed using GraphPad statistical software and version 9.4 of the SAS System for Windows (© 2002-2012 SAS Institute, Inc., Cary, NC, USA).

Results

Study population

We identified 206 patients with CBF-AML who were evaluated at our institution during the study period. Of these 206 patients, 114 had MRD monitoring performed and were included in our analysis. We excluded 92 patients who did not have MRD monitoring available because of death during treatment (n=19), administration of non-intensive or supportive care only (n=12), allogeneic BMT performed in first complete remission (n=17), MRD monitoring conducted at an outside institution (n=24), or for unspecified reasons (n=20) (Figure 1).

The median age of the 114 patients was 46.5 years (range, 18-79) and 44.7% were female. The t(8;21) was present in 58.8% and inv(16)/t(16;16) in 41.2% of patients. Over a median follow-up time of 3.7 years (range, 0.2-14.3), MRD measurements were performed a mean (± standard deviation, SD) 4.9±3.2 times per patient with a median (range) sampling interval of 98 (0-378) days. A total of 564 MRD measurements were performed, of which 77.5% within 120 days of the previous MRD sample. Most MRD measurements were performed on BM samples and 13.7% were performed on PB.

Figure 1.Flow diagram of patients with core-binding factor acute myeloid leukemia. CBF-AML: core-binding factor acute myeloid leukemia; MRD: measurable residual disease, BMT: bone marrow transplantation; CR1: first complete remission.

Risk of relapse

All patients except one (99.1%) achieved complete remission with standard induction chemotherapy; the single patient with refractory disease achieved complete remission with re-induction chemotherapy. Long-term remission was maintained in 71 (62.3%) patients but 43 (37.7%) relapsed, of whom 34 developed morphological relapse and nine had isolated molecular relapse but did not progress to morphological relapse because of pre-emptive treatment with allogeneic BMT or death from complications of treatment or disease. Most relapses occurred early with a median time from complete remission to molecular or morphological relapse of 9.1 months (range, 1.6-38.6); only 2/43 (4.7%) relapses occurred >2 years after the achievement of complete remission. The risk of relapse was significantly higher in patients who achieved <3 log reduction in RUNX1-RUNX1T1 or CBFB-MYH11 transcripts at the end of consolidation chemotherapy compared to patients who achieved ≥3 log reduction (61.1% vs. 33.7%, P=0.004) (Figure 2). Patients with <4 log reduction in MRD transcripts at the end of treatment also had a higher risk of relapse compared to patients with ≥4 log reduction (51.2% vs. 29.3%, P=0.026). Furthermore, relapsed patients were significantly less likely to have achieved MRD-negative status at the end of chemotherapy (23.1% vs. 45.2%, P=0.025) or complete molecular remission during follow-up (7.0% vs. 74.6%, P<0.0001), compared to patients who did not relapse (Table 1). There was no significant association between risk of relapse and MRD log reduction at the end of induction or with other clinical, cytogenetic, or genetic variables. There was a trend, which bordered on statistical significance, towards more extramedullary disease in patients with relapse (Table 1).

Of the 71 patients who did not relapse, 28 (39.4%) had persistent molecular disease during follow-up with detectable MRD at low transcript levels but with <1 log increase between two successive positive samples. Persistent molecular disease continued for a median of 1.5 years and up to a maximum of 3.4 years from the time of complete remission. Of these 28 patients, 13 eventually achieved MRD-negative status and 15 had persistent molecular disease to the end of follow-up MRD monitoring.

Relapse kinetics

Of the 43 patients who relapsed, the majority (74.4%, n=32) had rapid relapse kinetics with <100 days from molecular to morphological relapse. Of the 32 patients with rapid relapse kinetics, 17 (53.1%) had simultaneous molecular and morphological relapse, meaning that they progressed from a MRD-negative status (n=3) or stable levels of MRD (n=14) on one sample to morphological relapse on the subsequent sample. The remaining 15/32 (46.9%) developed molecular relapse prior to morphological relapse, but progression occurred rapidly over a median of 48 days (range, 11-95) such that no pre-emptive therapy, including allogeneic BMT, could be administered. Of the patients with rapid relapse kinetics, 16 (50.0%) received re-induction chemotherapy at the time of morphological relapse followed by allogeneic BMT. Eight patients (25.0%) received additional chemotherapy but did not proceed to allogeneic BMT and died from complications of chemotherapy or disease. Five (15.6%) patients died without further chemotherapy and three (9.4%) were lost to follow-up.

Figure 2.Kaplan-Meier analysis of relapse-free survival, according to reduction in measurable residual disease at end of chemotherapy. MRD: measurable residual disease.

MRD monitoring enabled timely detection of molecular relapse and permitted intervention prior to morphological relapse in only 11 patients (25.6%). Of these patients with slower relapse kinetics, eight (72.7%) went on to receive allogeneic BMT and three (27.3%) received additional chemotherapy but died from complications of chemotherapy or disease prior to allogeneic BMT. Pre-emptive intervention with allogeneic BMT at the time of molecular relapse prevented the development of morphological relapse in 6/11 (54.5%) patients. The median overall survival was 33.6 months for patients with slower relapse kinetics compared to 21.7 months for patients with rapid relapse kinetics (P=0.070).

There were no clinical features, molecular mutations, or cytogenetic abnormalities that significantly predicted patients with rapid versus slower relapse kinetics (Table 2). Furthermore, our inability to predict most relapses did not appear to be due to differences in MRD sampling technique, as there was no significant difference between patients with rapid and slower relapse kinetics in terms of the median time interval between MRD samples (70.5 vs. 70.0 days, P=0.74), the proportion of delayed MRD measurements (7.1% vs. 12.5%, P=0.35), or the number of MRD tests performed on PB (10.0% vs. 8.3%, P=1.0). Among patients who had a rise in MRD transcripts detected prior to the emergence of morphological relapse, a confirmatory sample was obtained within 4 weeks in 8/17 (47.1%) cases in the rapid relapse group (median 31 days; range, 3-91) and in 5/8 (62.5%) cases in the slower relapse group (median 28 days; range, 8-185), with no significant difference in time to confirmatory sample between the two groups (P=0.68). The time period during which relapses occurred was also similar between the two groups, with 20/32 (62.5%) relapses occurring after 2010 in the rapid relapse group compared to 5/11 (45.5%) in the slower relapse group (P=0.48).

Finally, a secondary analysis restricted to patients who achieved an optimal molecular response (defined here as ≥3 log reduction in MRD at the end of chemotherapy) yielded similar findings as our primary analysis. The majority of these patients (67.9%, n=19) experienced rapid progression from molecular to morphological relapse (median 11 days; range, 0-95), and MRD monitoring enabled the timely detection of impending relapse in only 9/28 (32.1%) of patients with an optimal molecular response.

Discussion

Although CBF-AML is associated with a higher rate of complete remission and a relatively favorable prognosis, up to 40% of patients develop relapse. Current international guidelines for CBF-AML recommend MRD monitoring every 3 months for 2 years after remission. This practice is intended to identify patients at risk of recurrence by detecting impending relapse and, presumably, to enable early intervention to prevent morphological relapse. However, our study demonstrates that this monitoring strategy fails to detect the majority of relapsing patients in a timely manner, as 74.4% of patients at our institution developed rapid morphological relapses such that no pre-emptive therapy, including allogeneic BMT, could be administered. In almost half these patients, molecular relapse occurred simultaneously with morphological relapse, while in the remaining cases the progression from molecular to morphological relapse occurred rapidly over a median of only 48 days. The current practice of measuring MRD every 3 months during follow-up therefore appears to provide insufficient lead-time to identify molecular relapses and intervene prior to morphological relapse. As a consequence, 114 patients in our study underwent serial MRD monitoring with BM sampling every 3 months for 24 months, but only 11 patients (9.6%) experienced a direct change in clinical management from this practice by receiving pre-emptive treatment prior to morphological relapse. This suggests that MRD monitoring may be of rare clinical utility in the follow-up of patients with CBF-AML.

The results of our study are significant given the lack of large longitudinal studies examining the clinical utility of serial MRD monitoring in CBF-AML. Our results are consistent with the long-term follow-up reported from the UK MRC AML-15 trial, in which serial MRD monitoring failed to predict impending relapse in 42/71 (59.2%) patients with morphological relapse.¹¹ However, the authors of that study attributed the inability to predict impending relapse to infrequent MRD measurements, as sampling intervals were >3 months in many patients. This contrasts with the generally good MRD monitoring adherence in our population of patients, among whom the median MRD sampling interval was 98 days. However, a limitation of our study is its real-world retrospective design, and we cannot exclude that delays in MRD sampling or variations in specimen quality may have limited our ability to predict impending relapse in some patients. Furthermore, the majority of relapses in our study occurred early at a median of 9 months after remission, which may have further limited the ability of serial MRD monitoring to predict relapse. Other studies have found serial MRD monitoring to be more strongly predictive of impending relapse. In a follow-up analysis of 94 patients enrolled in the French CBF-2006 trial who underwent MRD monitoring of PB every 3 months after remission, morphological relapse was predicted by persistent MRD positivity or molecular relapse in 21/29 (72.4%) cases.²¹ However, it is unclear if the timelier detection of impending relapse in that study translated into early intervention and improved clinical outcomes, as the cumulative incidence of morphological relapse remained high at 74.5% among the 23 patients with molecular relapse.

Table 1.Analysis of patients with long-term remission versus relapse.

This issue is further complicated by incomplete understanding of the relapse kinetics and optimal sampling interval of MRD measurements in CBF-AML. The current recommendation of MRD monitoring every 3 months is derived from the UK MRC AML-15 trial which reported a median time from molecular relapse in the BM to morphological relapse of 4.9 months in cases with t(8;21) and 3 months in those with inv(16).¹¹ Similarly, the CBF-2006 trial demonstrated a median time from molecular to morphological relapse of 3.9 months.²¹ However, other studies have described slower relapse kinetics with a median time from molecular to morphological relapse of up to 6 months or even 1 year in patients with inv(16).^22,23 Alternatively, other analyses are consistent with the rapid relapse kinetics observed in our study, with reported median times from molecular to morphological relapse of 35 to 60 days in small studies.^24,25 The discordant relapse kinetics across studies may be attributable to differences in sampling source (i.e., PB or BM), variable sensitivity of individual RT-qPCR assays, or differing MRD sampling intervals.²⁶ Individual patient-level factors may also contribute to differences in relapse kinetics, although our study did not identify any clinical variables significantly predictive of rapid relapse kinetics. Additional research is therefore warranted to better characterize the relapse kinetics of CBF-AML and to determine whether more frequent MRD sampling (e.g., PB every 4-8 weeks) might be more predictive of impending relapse.

Table 2.Analysis of patients with rapid versus slow relapse kinetics.

An additional area of uncertainty and interest is whether serial MRD monitoring can be used to inform treatment decisions. Currently, the European LeukemiaNet does not formally recommend changing therapy based on MRD status in CBF-AML due to lack of supportive data.⁶ However, the AML05 trial demonstrated that utilizing MRD status to identify high-risk patients with t(8;21) (i.e., those with <3 log reduction in MRD after the second cycle of consolidation or loss of molecular response within 6 months) as candidates for escalation of therapy to allogeneic BMT in first complete remission may lead to improved clinical outcomes. ²⁷ The GIMEMA AML1310 study also provides support for a risk-adapted, MRD-directed treatment strategy, as patients with intermediate-risk AML who were MRDpositive at the end of the first cycle of consolidation chemotherapy were referred for allogeneic BMT and achieved similar disease-free and overall survival rates as those with favorable-risk AML.²⁸ In the present study, preemptive intervention with allogeneic BMT at the time of molecular relapse prevented the development of morphological relapse in 6/11 patients with slower relapse kinetics. However, this finding requires confirmation in other populations of patients given the relatively small size and retrospective design of our study. The role for pre-emptive chemotherapy or allogeneic BMT in CBF-AML is also uncertain because most patients with morphological relapse respond well to salvage treatments.²⁶ Future studies should examine the clinical outcomes of different treatment strategies (e.g., observation or pre-emptive chemotherapy, immunotherapy, or BMT) for patients with CBF-AML in molecular relapse.

Although our study raises questions about the utility of serial MRD monitoring during follow-up after remission, we did identify the prognostic importance of measuring MRD at the end of treatment. In our study population, achievement of ≥3 log reduction in RUNX1-RUNX1T1 or CBFB-MYH11 transcripts at this time-point was associated with a significantly lower risk of relapse. This is consistent with the findings of other trials, including the UK MRC AML-15, AML05, and CBF-2006 trials, in which achievement of deep reductions in MRD transcript levels after induction or consolidation chemotherapy was associated with improved relapse-free survival.^11,12,27,29 Our study did not analyze the prognostic significance of measuring MRD after the second cycle of chemotherapy as recommended by the European LeukemiaNet, as the local institutional policy during this study period recommended measuring MRD after induction and the final cycle of consolidation chemotherapy. Other studies have identified additional potential prognostic factors that may predict relapse and poor outcomes in CBF-AML, including older age, elevated white blood cell count at diagnosis, extramedullary disease, secondary AML, additional cytogenetic changes (e.g., loss of chromosome X or Y, trisomy 22, deletion of 9q), and genetic mutations (e.g., KIT, FLT3, RAS, ASXL).³

In conclusion, measuring MRD at the end of treatment is predictive of relapse risk in CBF-AML. However, the current guideline recommendation of MRD monitoring every 3 months during follow-up failed to detect the majority of molecular relapses with sufficient lead-time to intervene and prevent morphological relapse in our study population. Further research is warranted to characterize the relapse kinetics of CBF-AML and to identify the patients at highest risk of relapse and the optimal strategies to monitor these patients over time.

Footnotes

• Received August 20, 2019
• Accepted January 2, 2020

Correspondence

AARON SCHIMMER aaron.schimmer@uhn.ca

Disclosures

ADS has received honorarium from Novartis, Jazz, and Otsuka Pharmaceuticals and research support from Medivir AB and Takeda. A.D.S. is named as an inventor on a patent application related to the use of DNT cells in AML. A.D.S. owns stock in AbbVie Pharmaceuticals. VG received honorarium, research funding through institution, and served on advisory board of Novartis and Incyte. CM has received honorarium from Novartis.

Contributions

RP performed research, performed data analysis, and wrote the manuscript. EA performed data analysis. JO, SC, VG, DM, CM, TM, AS, KY, HS, MM, CW, TS, and SK contributed to performing research and writing the manuscript. AS supervised the study and contributed to writing the manuscript.