AbstractSystemic lupus erythematosus is frequently associated with antiphospholipid syndrome. Patients with lupus-antiphospholipid syndrome are characterized by recurrent arterial/venous thrombosis, miscarriages, and persistent presence of autoantibodies against phospholipid-binding proteins, such as β2-Glycoprotein I. We investigated the cytokine production induced by β2-Glycoprotein I in activated T cells that infiltrate in vivo atherosclerotic lesions of lupus-antiphospholipid syndrome patients. We examined the helper function of β2-Glycoprotein I-specific T cells for tissue factor production, as well as their cytolytic potential and their helper function for antibody production. Lupus-antiphospholipid syndrome patients harbor in vivo activated CD4+ T cells that recognize β2-Glycoprotein I in atherosclerotic lesions. β2-Glycoprotein I induces T-cell proliferation and expression of both Interleukin-17/Interleukin-21 and Interferon-γ in plaque-derived T-cell clones. β2-Glycoprotein I-specific T cells display strong help for monocyte tissue factor production, and promote antibody production in autologous B cells. Moreover, plaque-derived β2-Glycoprotein I-specific CD4+ T lymphocytes express both perforin-mediated and Fas/FasLigand-mediated-cytotoxicity. Altogether, our results indicate that β2-Glycoprotein I is able to elicit a local Interleukin-17/Interleukin-21 and Interferon-γ inflammation in lupus-antiphospholipid syndrome patients that might lead, if unabated, to plaque instability and subsequent arterial thrombosis, suggesting that the T helper 17/T helper 1 pathway may represent a novel target for the prevention and treatment of the disease.
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that is frequently associated with antiphospholipid syndrome (APS) characterized by recurrent vascular thrombosis and pregnancy morbidities associated with the persistent presence of autoantibodies against phospholipid-binding proteins, namely antiphospholipid antibodies (aPL), such as β2-glycoprotein I (β2GPI).1 Besides its role in the acquired pro-coagulant diathesis, aPL have been also associated with accelerated atherosclerosis to explain cardiovascular manifestations of the syndrome.42 An accelerated atherosclerosis in SLE was first demonstrated in 1975 by Bulkley et al.5 in a necroscopic study, that was further confirmed by Urowitz et al.6
Many studies showed that SLE is associated with coronary heart disease and atherosclerosis;97 an important prospective study demonstrated that SLE patients have an accelerated progression of carotid plaque formations compared to non-lupus controls.10 SLE patients have a reduced life expectancy mainly due to the increased prevalence of cardiovascular diseases. Incidence of major cardiovascular events is 2.5 times higher in SLE patients compared to the general population. Compared to healthy subjects, SLE women, aged 35-44 years, have a 50 times increased risk of myocardial infarction and accelerated atherosclerosis, that is a well recognized comorbidity in SLE.1211
Atherosclerosis is a multifactorial disease for which a number of different pathogenic mechanisms have been proposed. In addition to classical risk factors, in the last two decades, attention has been focused on inflammatory processes.1413 Observations in humans and animals suggest that atherosclerotic plaques derive from specific cellular and molecular mechanisms that can be ascribed to an inflammatory disease of the arterial wall, the lesions of which consist of activated macrophages and T lymphocytes. If inflammation continues unabated, it results in an increased number of plaque-infiltrating macrophages and T cells, which contribute to the remodeling of the arterial wall, eventually favoring plaque instability and rupture.15 Within the T-cell population infiltrating the plaque, most cells are activated CD4 T helper (Th) 1 and Th17 cells expressing HLA-DR and the interleukin (IL)-2 receptor (CD25).1716
Current evidence indicates that autoimmunity can be detected within the atherosclerotic lesions.18 Accordingly, self-phospholipids, such as oxidized low-density lipoprotein (oxLDL) and human heat shock proteins, drive T-cell inflammation in atherosclerotic patients.2019 However, the multifactorial nature of atherosclerosis suggests that a larger number of autoantigens might be involved.
It has been hypothesized that the development of an anti-β2GPI-specific response in the target organ may con tribute to atherothrombosis in SLE-APS patients. This hypothesis is largely based on the β2GPI presence in human atherosclerotic plaques2221 and on the enhanced fatty streak formation in transgenic atherosclerosis-prone mice immunized with β2GPI.2423 Moreover, β2GPI-reactive T cells have also been found to promote early atherosclerosis in LDL receptor deficient mice.25
In this study, we demonstrate that, in SLE-APS patients, both IL-17 and IFN-γ are secreted by atherosclerotic plaques infiltrating Th cells in response to β2GPI, and suggest that β2GPI drives a local Th17/Th1 inflammatory response, which can be responsible for plaque instability and rupture, leading to atherothrombosis.
A detailed description of the methods is available in the Online Supplementary Appendix.
Human β2GPI was purified as described.26 We ruled out the presence of contaminants by a limulus test. The human β2GPI used was with a limulus test and resulted negative throughout the whole study.
Upon approval of the local Ethical Committee, the following patients were enrolled in the study: ten patients (10 females; mean age 51 years, range 42-56 years) with SLE-APS, ten aPL negative patients (10 females; mean age 51 years, range 43-55 years), five SLE aPL-positive patients (5 females; mean age 49 years, range 44-53 years), and five SLE aPL-negative patients (5 females; mean age 50 years, range 44-56 years); all were affected by carotid atherosclerotic arteriopathy. The carotid plaques were obtained by endoarterectomy from each patient. The clinical information of each patient is reported in Online Supplementary Tables S1-S4.
All patients studied (SLE-APS, SLE aPL-positive, SLE aPL-negative, and aPL negative patients) were eligible for vascular surgery. All the SLE aPL-positive patients were affected by SLE but not by APS, although they were positive for aPL, with serum anti-β2GPI, anti-cardiolipin antibodies or with positivity for LA. All SLE aPL-neg patients were affected by SLE but not by APS, and they were triple negative for serum aPL, such as anti-β2GPI, anti-cardiolipin antibodies and with negativity for Lupus Anticoagulant.
Anti-phospholipid antibody detection
The detection of aCL and aβ2GPI in patient sera, and analysis of LA was performed as described elsewhere.2928
Generation and characterization of T-cell clones from atherosclerotic plaques inflammatory infiltrates
Carotid specimens, obtained by endoarterectomy, were investigated in both SLE-APS and in aPL negative patients under the same experimental conditions. Specimens were then disrupted, and single T cells were cloned under limiting dilution, as described.16 To assess their phenotype profile, T-cell clones were screened by flow cytometry with fluorochrome-conjugated anti-CD3, anti-CD4, anti-CD8 on a BD FACSCanto II (BD Bioscience), using the FACS Diva 6.1.3. software. The repertoire of the TCR Vβ chain of β2GPI-specific Th clones was analyzed with a panel of mAb specific to the following: Vβ1, Vβ2, Vβ4, Vβ5.1, Vβ5.2, Vβ5.3, Vβ7, Vβ8, Vβ9, Vβ11, Vβ12, Vβ13.1, Vβ13.2 and Vβ13.6, Vβ14, Vβ16, Vβ17, Vβ18, Vβ20, Vβ21.3, Vβ22, and Vβ23 (Beckman Coulter); Vβ6.7 (Gentaur) and Vβ3.1 (In Vitro Gen). Isotype-matched non-specific Ig were used as negative control. Vβ10, Vβ15, and Vβ19 T-cell receptor typing were investigated by Clontech kit, according to the manufacturer’s instructions. Each β2GPI-reactive CD4 T-cell clone was stained by only one of the TCR-Vβ chain–specific monoclonal antibodies, showing a single peak of fluorescence intensity (Online Supplementary Figure S1). The cytokine production, the cytotoxicity, the helper functions for antibody and tissue factor production of β2GPI-specific T-cell clones were performed as described.313016
Statistical analyses were performed using Student’s t-test. P<0.05 was considered significant.
Atherosclerotic lesions of systemic lupus erythematosus patients with antiphospholipid syndrome and systemic lupus erythematosus patients positive for antiphospholipid antibodies harbor autoreactive β2GPI-specific CD4+ T-cell clones
Atherosclerotic plaque-infiltrating in vivo activated T cells were expanded in vitro in an hrIL-2 conditioned medium, subsequently cloned and studied for their phenotypic and functional profile. A total number of 297 CD4 and 37 CD8 T-cell clones were obtained from atherosclerotic lesions of ten SLE-APS patients. For each patient, CD4 and CD8 atherosclerotic lesion-derived T-cell clones were assayed for proliferation in response to medium, or β2GPI. None of the CD8 T-cell clones showed proliferation to β2GPI although they proliferated in response to mitogen stimulation (Figure 1). We have also investigated the amount of β2GPI-specific T cells present in the peripheral blood of SLE-APS patients and compared it with the one found in atheromas. The proportion of β2GPI-specific CD4 T-cell clones generated from atherosclerotic plaques of SLE-APS patients was 24%, which is remarkably higher than the frequency of β2GPI-specific T cells found in the peripheral blood of the same patients (between 1:1900 and 1:3400).
Seventy-one (24%) of the 297 CD4 T-cell clones generated from SLE-APS atherosclerotic plaque-infiltrating T cells proliferated significantly to β2GPI (Figure 1). Each SLE-APS patient displayed a comparable percentage of CD4 T-cell clones responsive to β2GPI (Online Supplementary Table S1). On the other hand, a total number of 288 CD4 and 42 CD8 T-cell clones were obtained from atherosclerotic lesions of ten atherothrombotic patients, that were negative for aPL. For each patient, CD4 and CD8 atherosclerotic lesion-derived T-cell clones were assayed for proliferation in response to medium or β2GPI. None of the CD4 or CD8 T-cell clones derived from the atherosclerotic lesions showed proliferation to β2GPI (Online Supplementary Table S2). A total number of 135 CD4 and 21 CD8 T-cell clones were obtained from atherosclerotic lesions of five SLE aPL-positive. For each patient, CD4 and CD8 atherosclerotic lesion-derived T-cell clones were assayed for proliferation in response to medium or β2GPI. 25 CD4 and no CD8 T-cell clones derived from the atherosclerotic lesions of SLE aPL-positive patients showed proliferation to β2GPI (Online Supplementary Table S3). A total number of 136 CD4 and 30 CD8 T-cell clones were obtained from atherosclerotic lesions of five SLE aPL-negative. For each patient, CD4 and CD8 atherosclerotic lesion-derived T-cell clones were assayed for proliferation in response to medium or β2GPI. None of the CD4 or CD8 T-cell clones derived from the atherosclerotic lesions showed proliferation to β2GPI (Online Supplementary Table S4).
All β2GPI-specific T-cell clones, both those obtained from the atherosclerotic lesions of SLE-APS patients and those obtained from SLE aPL-positive patients, were stimulated with β2GPI and autologous APC. Then, TNF-α and IL-4, IFN-γ and IL-17 production was measured in culture supernatants. Upon antigen stimulation with β2GPI of the 71 β2GPI-specific T-cell clones obtained from SLE-APS patients, 30 were polarized Th1 clones, 10 Th clones were Th17, 27 Th clones were Th17/Th1, and only 4 were able to produce IL-4 together with TNF-α (Th0 clones) (Figure 2). Upon antigen stimulation with β2GPI of the 25 β2GPI-specific T-cell clones obtained from SLE aPL-positive patients, 10 were polarized Th1 clones, 6 Th clones were polarized Th17, 8 Th clones were Th17/Th1, and only one was Th0 (Figure 3). T-cell blasts from each of the 71 β2GPI-reactive T-cell clones obtained from atherosclerotic lesions of patients with SLE-APS were further screened by IFN-γ and IL-17 ELISPOT in response to β2GPI. Upon appropriate stimulation, 61 atherosclerotic-derived CD4 T-cell clones produced IFN-γ, and thirty-seven produced IL-17 (Figure 4). Interestingly, all IL-17-producing β2GPI-reactive T-cell clones, produce IL-21 (mean ± SE, 3.3 ± 0.5 ng/mL per 10 T cells) in response to antigen stimulation.
β2GPI-specific atherosclerotic lesion-infiltrating T cells help monocyte tissue factor production and procoagulant activity
Plaque rupture and consequent thrombosis are crucial complications of atherosclerosis. TF plays a key role in triggering atherothrombotic events being the primary activator of the coagulation cascade. We investigated whether atherosclerotic lesion-infiltrating β2GPI-specific T cells had the potential to express helper functions for TF production and PCA by autologous monocytes. Antigen-stimulated β2GPI-specific atherosclerotic lesion-derived T-cell clones were co-cultured with autologous monocytes and levels of TF and PCA were measured. Antigen stimulation resulted in the expression of substantial help for TF (Figure 5A) production and PCA (Figure 5B) by autologous monocytes.
Atherosclerotic lesion-derived β2GPI-specific T-cell clones express antigen-dependent help to autologous B cells for Ig production
T/B-cell interaction is a multistep process resulting in B-cell help depending on the functional commitment of the Th cells involved. So far the ability of SLE-APS-derived β2GPI-specific T-cell clones to provide B-cell help for Ig synthesis has been investigated. In the absence of the specific antigen, no increase in IgM, IgG, or IgA production above spontaneous levels measured in cultures containing B cells alone was observed. In the presence of β2GPI and at a T-to-B cell ratio of 0.2 to 1, all of the β2GPI-specific T-cell clones provided substantial help for Ig production. At a 1:1 T/B cell ratio, β2GPI-dependent T-cell help for IgM, IgG, and IgA production by B cells was much higher (Figure 6). However, at a 5:1 T/B cell ratio, co-culturing B cells with autologous β2GPI-specific T-cell clones in the presence of β2GPI resulted in a much lower Ig synthesis.
Atherosclerotic lesion-derived β2GPI-specific T-cell clones display cytotoxic and pro-apoptotic activity
The cytolytic potential of SLE-APS-derived atherosclerotic lesion-derived β2GPI-specific autoreactive T-cell clones was assessed by using antigen-pulsed Cr-labeled autologous EBV-B cells as targets. At an E:T ratio of 10:1, all Th1 and Th17/Th1 specific T-cell clones were able to lyse β2GPI-presenting autologous Epstein-Barr virus (EBV)-B cells (range of specific 51Cr release, 35-65%), whereas autologous EBV-B cells pulsed with control ag and co-cultured with the same clones were not lysed (Figure 7A). Likewise 2 Th0 and all Th17 specific T-cell clones were able to lyse their target (specific Cr release: 50% and 25-45% respectively), while no lysis was observed when using autologous EBV-B cells pulsed with the control ag.
Fas-FasL mediated apoptosis was assessed using Fas Jurkat cells as target. T-cell blasts from each clone were co-cultured with 51Cr-labeled Jurkat cells at an E:T ratio of 10, 5, and 2.5 to 1 for 18 h in the presence of PMA and ionomycin (Figure 7B). Upon mitogen activation, 27 out of 30 Th1, 24/27 Th17/Th1, 4/10 Th17, and 2 out of 4 Th0 clones were able to induce apoptosis in target cells (range of specific Cr release: 25-61%).
Several clinical studies and experimental models suggest a role for aPL in accelerating atherosclerotic plaque formation in SLE. On the other hand, there is growing evidence that aPL represent a risk factor for arterial thrombosis supporting their pathogenic role in cardiovascular events.32431 Here, we report for the first time that a pro-inflammatory and pro-coagulant β2GPI-specific Th17, Th1 and Th17/Th1 infiltrate in human atherosclerotic lesions of patients with SLE-APS and may represent a key pathogenic atherothrombotic mechanism.
Many self antigens, such as oxLDL, may theoretically be involved in SLE-APS atherosclerosis; oxLDL-specific peripheral blood-derived T cells displaying a Th1 profile were reported in APS patients.33 However, there is no information on whether these cells are actively involved in atherosclerotic tissue lesions of SLE-APS patients. In addition, β2GPI was found to bind ox-LDL34 raising the issue of whether or not the immune response is against ox-LDL or β2GPI itself.
The relevance of the data presented in this paper consists in the demonstration that all ten SLE-APS patients with clinically severe atherothrombosis harbored in their target tissues, such as atherosclerotic lesions, in vivo-activated CD4 T cells able to react to β2GPI. CD4 T cells specific for β2GPI were found also in the plaques of SLE aPL-positive patients but not in SLE aPL-negative patients nor in atherosclerotic patients without SLE. The results suggested that β2GPI drive inflammation in atherosclerotic plaques in SLE-APS and SLE aPL-positive patients, while in SLE aPL-negative patients and in non-SLE patients other antigens are involved in sustaining plaque inflammation. With the experimental procedure used in this study, the proportion of β2GPI-specific CD4 T-cell clones generated from atherosclerotic plaques of atherothrombotic SLE-APS patients is remarkably higher than the frequency of β2GPI-specific T cells found in their peripheral blood.
In order to investigate plaque instability, we investigated fresh T cells coming from the atherosclerotic plaques of SLE-APS patients and we found that plaque-derived CD4 T cells specifically produce IFN-γ and IL-17 in response to both β2GPI and to mitogen stimulation. Studying at clonal level the β2GPI-specific T cells found in the inflammatory atherosclerotic infiltrates of SLE-APS we found that 42% were polarized T helper 1 cells, 38% were Th17/Th1 cells, 15% were polarized Th17 cells, 5% were Th0 cells, and no T cells were polarized Th2 cells. The lack of Th2 cells is an important risk factor in the genesis of atherosclerosis. Indeed, T cells play an important role in the genesis of atherosclerosis that has been defined a Th1-driven immunopathology,3635 and we have demonstrated that Th1 cells, producing high levels of IFN-γ, are crucial for the development of the disease.222016 Given that atherosclerosis can occur and progress even in IFN-γ- or IFN-γR–deficient mice, although with a lower lesion burden,37 other Th cells and factors are presumably involved in the genesis of the atheroma. A third subset of effector Th cells, namely Th17, has been discovered.38 Th17 cells are potent inducers of tissue inflammation and have been associated with the pathogenesis of many experimental autoimmune diseases and human inflammatory conditions.4039 In the lymphocytic infiltrates of SLE-APS atherosclerotic plaques, we have found the presence of in vivo-activated plaque-infiltrating T cells able to produce IL-17 and IL-21 in response to β2GPI. Among the clonal progeny of T cells infiltrating the lesions, we demonstrated the presence of β2GPI -specific T cells able to secrete IL-17. A significant number (27%) of IL-17–producing T cells are also IFN-γ producers. This finding is in agreement with a previous report that demonstrated the concomitant production of IL-17 and IFN-γ by human coronary artery-infiltrating T cells in non SLE patients.4341 Plaque rupture and thrombosis are notable complications of atherosclerosis.4316 The methodology used to obtain the plaque derived T cells encompasses a clonal expansion step, followed by limiting dilution to obtain single clones. The β2GPI-reactive CD4+ T-cell clone found in atherosclerotic plaques were unique, based on the T-cell receptor - Vβ results obtained in the study. The β2GPI -specific T-cell clones revealed their ability, not only to induce macrophage production of TF upon antigen stimulation, but that they were also able to promote PCA.
Th17 cells were shown to play a key role in experimental mouse models of atherosclerosis; IL-17 is proatherogenic in an experimental model of accelerated atherosclerosis in the presence of a high fat diet (HFD).44 In fact, in IL-17 mice fed with HFD, the aortic lesion size and lipid composition as well as macrophage accumulation in the plaques were significantly diminished, and the progression of the process was remarkably reduced compared with WT mice. Furthermore IL-21 was produced by almost all Th1 and Th17/Th1 cells specific for β2GPI. IL-21 is actually up-regulated in patients with peripheral artery diseases.45 Expression of IL-17 in human atherosclerotic lesions is associated with increased inflammation and plaque vulnerability, and increased Th17 cells.46 An increased incidence of atherosclerosis associated with peripheral blood Th17 responses has been demonstrated in patients with SLE.47
We have demonstrated that β2GPI was able to activate Th17 and Th1 responses in atherosclerotic lesions of SLE-APS patients. The relevance of Th17/Th1 cells in non-SLE-atherosclerosis patients have been demonstrated in other studies,4948 suggesting that Th1 and Th17 cells might plastically shift into each other in different phases of the disease. It has been shown that Th17 cells might shift towards Th1 but not to Th2 via IL-12 receptor signaling.50
Overall, our findings support the concept that a crucial component of atherosclerosis in SLE-APS is represented by T-cell-mediated immunity and that chronic Th response against β2GPI plays an important role in the genesis of atheroma in SLE-APS patients.51 Among β2GPI-specific IL-17–producing Th cells, the majority were polarized Th17 cells, whereas others were able to produce both IFN-γ and IL-17. Thus, it is possible to speculate that Th17 and Th1 cells co-migrate to the inflamed tissue and cooperate in the ongoing inflammatory process within the atherosclerotic lesion.52391716
In addition, upon appropriate Ag stimulation, the majority of atherosclerotic plaque-derived β2GPI-specific clones induced both perforin-mediated cytolysis and Fas/FasL-mediated apoptosis in target cells and were able to drive the upregulation of TF production by monocytes within atherosclerotic plaques, thus further contributing to the thrombogenicity of lesions.534342 Our results demonstrate that β2GPI is a major factor able to drive Th17 and Th1 inflammatory process in SLE-APS atherosclerosis, and suggest that Th17/Th1 cell pathway and β2GPI may represent important targets for the prevention and treatment of the disease.
- ↵Check the online version for the most updated information on this article, online supplements, and information on authorship & disclosures: www.haematologica.org/content/104/12/2519
- FundingThis research has been supported by grants from Italian Ministry of University and Research and Italian Ministry of Health (MMDE), IRCCS Istituto Auxologico Italiano, Ricerca Corrente (PLM).
- Received October 18, 2018.
- Accepted March 6, 2019.
- Meroni PL, Chighizola CB, Rovelli F. Antiphospholipid syndrome in 2014: more clinical manifestations, novel pathogenic players and emerging biomarkers. Arth Res Ther. 2014; 16(2):209-225. Google Scholar
- Hügli RW, Gremmelmaier D, Jeanneret C. Unusual vascular focal high-grade arterial stenoses in a young woman with systemic lupus erythematosus and secondary antiphospholipid syndrome. Lupus. 2011; 20(3):311-314. PubMedhttps://doi.org/10.1177/0961203310383069Google Scholar
- Ames PR, Margarita A, Alves JD. Antiphospholipid Antibodies and Atherosclerosis: Insights from Systemic Lupus Erythematosus and Primary Antiphospholipid Syndrome. Clinic Rev Allerg Immunol. 2009; 37(1):29-35. Google Scholar
- Hollan H, Meroni PL, Ahearn JM. Cardiovascular diseases in autoimmune rheumatic diseases. Autoimmunity Reviews. 2013; 12(10):1004-1015. PubMedhttps://doi.org/10.1016/j.autrev.2013.03.013Google Scholar
- Bulkley BH, Roberts WC. The heart in systemic lupus erythematosus and the changes induced in it by corticosteroid therapy: a study of 36 necroscopy cases. Am J Med. 1975; 53(2):243-264. Google Scholar
- Urowitz MB, Bookman AA, Koehler BE. The bimodal mortality pattern of SLE. Am J Med. 1976; 60(2):221-225. PubMedhttps://doi.org/10.1016/0002-9343(76)90431-9Google Scholar
- Petri M, Perez-Gutthann S, Spence D. Risk factors for coronary artery disease in patients with systemic lupus erythematosus. Am J Med. 1992; 93(5):513-519. PubMedhttps://doi.org/10.1016/0002-9343(92)90578-YGoogle Scholar
- Esdaile JM, Abrahamowicz M, Grodzicky T. Traditional Framingham risk factors fail to fully account for accelerated atherosclerosis in systemic lupus erythematosus. Arthritis Rheum. 2001; 44(10):2331-2337. PubMedhttps://doi.org/10.1002/1529-0131(200110)44:10<2331::AID-ART395>3.0.CO;2-IGoogle Scholar
- Manzi S, Selzer F, Sutton-Tyrrell K. Prevalence and risk factors of carotid plaque in women with systemic lupus erythematosus. Arthritis Rheum. 1999; 42(1):51-60. PubMedhttps://doi.org/10.1002/1529-0131(199901)42:1<51::AID-ANR7>3.0.CO;2-DGoogle Scholar
- Thompson T, Sutton-Tyrrell K, Wildman RP. Progression of carotid intima- media thickness and plaque in women with systemic lupus erythematosus. Arthritis Rheum. 2008; 58(3):835-842. PubMedhttps://doi.org/10.1002/art.23196Google Scholar
- McMahon M, Hahn BH. Atherosclerosis and systemic lupus erythematosus: mechanistic basis of the association. Curr Opin Immunol. 2007; 19(6):633-639. PubMedhttps://doi.org/10.1016/j.coi.2007.11.001Google Scholar
- Shoenfeld Y, Gerli R, Doria A. Accelerated atherosclerosis in autoimmune rheumatic diseases. Circulation. 2007; 112(21):3337-3347. Google Scholar
- Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature. 1993; 362(6423):801-809. PubMedhttps://doi.org/10.1038/362801a0Google Scholar
- Ross R. Atherosclerosis: an inflammatory disease. N Engl J Med. 1999; 340(2):115-126. PubMedhttps://doi.org/10.1056/NEJM199901143400207Google Scholar
- Hansson GK, Jonasson L, Seifert PS. Immune mechanisms in atherosclerosis. Arteriosclerosis. 1989; 9(5):567-578. PubMedhttps://doi.org/10.1161/01.ATV.9.5.567Google Scholar
- Benagiano M, Azzurri A, Ciervo A. T helper type-1 lymphocyte-driven inflammation in human atherosclerotic lesions. Proc Natl Acad Sci U S A. 2003; 100(11):6658-6663. PubMedhttps://doi.org/10.1073/pnas.1135726100Google Scholar
- Benagiano M, Munari F, Ciervo A. Chlamydophila pneumoniae phospholipase D (CpPLD) drives Th17 inflammation in human atherosclerosis. Proc Natl Acad Sci USA. 2012; 109(4):1222-1227. PubMedhttps://doi.org/10.1073/pnas.1111833109Google Scholar
- Wick G, Knoflach M, Xu Q. Autoimmune and inflammatory mechanisms in atherosclerosis. Ann Rev Immunol. 2004; 22:361-403. PubMedhttps://doi.org/10.1146/annurev.immunol.22.012703.104644Google Scholar
- Stemme S, Faber B, Holm J. T lymphocytes from human atherosclerotic plaques recognize oxidized low density lipoprotein. Proc Natl Acad Sci U S A. 2005; 92(9):3893-3897. Google Scholar
- Benagiano M, D’Elios MM, Amedei A. Human 60-kDa heat shock protein is a target autoantigen of T cells derived from atherosclerotic plaques. J Immunol. 2005; 174(10):6509-6517. PubMedhttps://doi.org/10.4049/jimmunol.174.10.6509Google Scholar
- George J, Harats D, Gilburd B. Immunolocalization of β2-glycoprotein I (Apolipoprotein H) to human atherosclerotic plaques: potential implications for lesion progression. Circulation. 1999; 99(17):2227-2230. PubMedhttps://doi.org/10.1161/01.CIR.99.17.2227Google Scholar
- Benagiano M, Gerosa M, Romagnoli J. β2 Glycoprotein I Recognition Drives Th1 Inflammation in Atherosclerotic Plaques of Patients with Primary Antiphospholipid Syndrome. J Immunol. 2017; 198(7):2640-2648. PubMedhttps://doi.org/10.4049/jimmunol.1600305Google Scholar
- George J, Harats D, Gilburd B. Induction of Early Atherosclerosis in LDL-receptor-deficient mice immunized with β2-glycoprotein I. Circulation. 1998; 98(11):1108-1115. PubMedhttps://doi.org/10.1161/01.CIR.98.11.1108Google Scholar
- Afek A, George J, Shoenfeld Y. Enhancement of atherosclerosis in β2-gly-coprotein I-immunized apolipoprotein E-deficient mice. Pathobiology. 1999; 67(1):19-25. PubMedhttps://doi.org/10.1159/000028046Google Scholar
- George J, Harats D, Gilburd B. Adoptive transfer of β2-glycoprotein I-reactive lymphocytes enhances early atherosclerosis in LDL receptor-deficient mice. Circulation. 2000; 102(15):1822-1827. PubMedhttps://doi.org/10.1161/01.CIR.102.15.1822Google Scholar
- Steinkasserer A, Estaller C, Weiss EH. Complete nucleotide and deduced amino acid sequence of human β2-glycoprotein I. Biochem J. 1991; 277(Pt 2):387-391. PubMedhttps://doi.org/10.1042/bj2770387Google Scholar
- Miyakis S, Lockshin MD, Atsumi T. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost. 2006; 4(2):295-306. PubMedhttps://doi.org/10.1111/j.1538-7836.2006.01753.xGoogle Scholar
- Meroni PL, Peyvandi F, Foco L. Anti-β2 glycoprotein I antibodies and the risk of myocardial infarction in young premenopausal women. J Thromb Haemost. 2007; 5(12):2421-2428. PubMedhttps://doi.org/10.1111/j.1538-7836.2007.02763.xGoogle Scholar
- Chighizola CB, Raschi E, Banzato A. The challenges of lupus anticoagulants. Expert Rev Hematol. 2016; 9(4):389-400. Google Scholar
- Helin H, Edgington TS. Allogenic induction of the human T cell-instructed monocyte procoagulant response is rapid and is elicited by HLA-DR. J Exp Med. 1983; 158(3):962. PubMedhttps://doi.org/10.1084/jem.158.3.962Google Scholar
- D’Elios MM, Bergman MP, Azzurri A. H(+),K(+)-atpase (proton pump) is the target autoantigen of Th1-type cytotoxic T cells in autoimmune gastritis. Gastroenterology. 2001; 120(2):377-386. PubMedhttps://doi.org/10.1053/gast.2001.21187Google Scholar
- Meroni PL, Borghi MO, Raschi E. Pathogenesis of antiphospholipid syndrome: understanding the antibodies. Nat Rev Rheumatol. 2011; 7(6):330-339. PubMedhttps://doi.org/10.1038/nrrheum.2011.52Google Scholar
- Laczik R, Szodoray P, Veres K. Oxidized LDL induces in vitro lymphocyte activation in antiphospholipid syndrome. Autoimmunity. 2010; 43(4):334-339. Google Scholar
- Matsuura E, Lopez LR, Shoenfeld Y. β2-glycoprotein I and oxidative inflammation in early atherogenesis: a progression from innate to adaptive immunity?. Autoimmun Rev. 2012; 12(2):241-249. PubMedhttps://doi.org/10.1016/j.autrev.2012.04.003Google Scholar
- Elhage R, Jawien J, Rudling M. Reduced atherosclerosis in interleukin-18 deficient apolipoprotein E-knockout mice. Cardiovasc Res. 2003; 59(1):234-240. PubMedhttps://doi.org/10.1016/S0008-6363(03)00343-2Google Scholar
- Lee TS, Yen HC, Pan CC. The role of interleukin 12 in the development of atherosclerosis in ApoE-deficient mice. Arterioscler Thromb Vasc Biol. 1999; 19(3):734-742. PubMedhttps://doi.org/10.1161/01.ATV.19.3.734Google Scholar
- Whitman SC, Ravisankar P, Daugherty A. J Interferon Cytokine Res. 2002; 22(6):661-670. PubMedhttps://doi.org/10.1089/10799900260100141Google Scholar
- Park H, Li Z, Yang XO. A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol. 2005; 6(11):1133-1141. PubMedhttps://doi.org/10.1038/ni1261Google Scholar
- Langrish CL, Chen Y, Blumenschein WM. IL-23 drives a pathogenic T cell popu lation that induces autoimmune inflammation. J Exp Med. 2005; 201(2):233-240. PubMedhttps://doi.org/10.1084/jem.20041257Google Scholar
- Korn T, Oukka M, Kuchroo V. IL-21 initiates an alternative pathway to induce proinflammatory T(H)17 cells. Nature. 2007; 448(7152):484-487. PubMedhttps://doi.org/10.1038/nature05970Google Scholar
- Zhu J, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations. Annu Rev Immunol. 2010; 28:445-489. PubMedhttps://doi.org/10.1146/annurev-immunol-030409-101212Google Scholar
- Eid RE, Rao DA, Zhou J. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation. 2009; 119(10):1424-1432. PubMedhttps://doi.org/10.1161/CIRCULATIONAHA.108.827618Google Scholar
- Toschi V, Gallo R, Lettino M. Tissue factor modulates the thrombogenicity of human atherosclerotic plaques. Circulation. 1997; 95(3):594-599. PubMedhttps://doi.org/10.1161/01.CIR.95.3.594Google Scholar
- Chen S, Shimada K, Zhang W. IL-17A is proatherogenic in high-fat diet-induced and Chlamydia pneumoniae infection-accelerated atherosclerosis in mice. J Immunol. 2010; 185(9):5619-5627. PubMedhttps://doi.org/10.4049/jimmunol.1001879Google Scholar
- Wang T, Cunningham A, Houston K. Endothelial interleukin-21 receptor upregulation in peripheral artery disease. Vasc Med. 2016; 21(2):99-104. PubMedhttps://doi.org/10.1177/1358863X15621798Google Scholar
- Erbel C, Dengler TJ, Wangler S. Expression of IL-17A in human atherosclerotic lesions is associated with increased inflammation and plaque vulnerability. Basic Res Cardiol. 2011; 106(1):125-134. PubMedhttps://doi.org/10.1007/s00395-010-0135-yGoogle Scholar
- Zhu M, Mo H, Li D. Th17/Treg imbalance induced by increased incidence of atherosclerosis in patients with systemic lupus erythematosus (SLE). Clin Rheumatol. 2013; 32(7):1045-1052. PubMedhttps://doi.org/10.1007/s10067-013-2237-zGoogle Scholar
- Zhang L, Wang T, Wang XQ. Elevated frequencies of circulating Th22 cell in addition to Th17 cell and Th17/Th1 cell in patients with acute coronary syndrome. PLoS One. 2013; 8(12):e71466. PubMedhttps://doi.org/10.1371/journal.pone.0071466Google Scholar
- Zhao Z, Wu Y, Cheng M. Activation of Th17/Th1 and Th1, but not Th17, is associated with the acute cardiac event in patients with acute coronary syndrome. Atherosclerosis. 2011; 217(2):518-524. PubMedhttps://doi.org/10.1016/j.atherosclerosis.2011.03.043Google Scholar
- Peck A, Mellins ED. Plasticity of T-cell phenotype and function: the T helper type 17 example. Immunology. 2010; 129(2):147-153. PubMedhttps://doi.org/10.1111/j.1365-2567.2009.03189.xGoogle Scholar
- Rauch J, Salem D, Subang R. β2-Glycoprotein I-Reactive T Cells in Autoimmune Disease. Front Immunol. 2018; 10:2836. Google Scholar
- Savino MT, Ulivieri C, Emmi G. The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development. J Leukoc Biol. 2013; 93(4):549-559. PubMedhttps://doi.org/10.1189/jlb.0712331Google Scholar
- Ketelhuth DF, Hansson GK. Adaptive response of T and B cells in atherosclerosis. Circ Res. 2016; 118(4):668-678. PubMedhttps://doi.org/10.1161/CIRCRESAHA.115.306427Google Scholar