The novel CD19-targeting antibody-drug conjugate huB4-DGN462 shows improved anti-tumor activity compared to SAR3419 in CD19-positive lymphoma and leukemia models

Abstract

Antibody-drug conjugates (ADC) are a novel way to deliver potent cytotoxic compounds to cells expressing a specific antigen. Four ADC targeting CD19, including SAR3419 (coltuximab ravtansine), have entered clinical development. Here, we present huB4-DGN462, a novel ADC based on the SAR3419 anti-CD19 antibody linked via sulfo-SPDB to the potent DNA-alkylating agent DGN462. huB4-DGN462 had improved in vitro anti-proliferative and cytotoxic activity compared to SAR3419 across multiple B-cell lymphoma and human acute lymphoblastic leukemia cell lines. In vivo experiments using lymphoma xenografts models confirmed the in vitro data. The response of B-cell lymphoma lines to huB4-DGN462 was not correlated with CD19 expression, the presence of BCL2 or MYC translocations, TP53 inactivation or lymphoma histology. In conclusion, huB4-DGN462 is an attractive candidate for clinical investigation in patients with B-cell malignancies.

Introduction

Lymphomas are among the most common cancers and causes of cancer-related cell deaths in both adults and children.1 In western countries, B-cell lymphomas, and diffuse large B-cell lymphoma (DLBCL) in particular, represent the vast majority of cases. Despite treatment improvements, a proportion of B-cell lymphoma patients still succumb because of chemorefractory disease, requiring the need to find novel therapeutic modalities to extend patients’ lives.32 These neoplastic B cells express many proteins on their cell surface that are potential therapeutic targets.4 The clinical success of the anti-CD20 monoclonal antibody, rituximab, demonstrates the potential of targeting B-cell specific surface proteins.5 Antibody-drug conjugates (ADC) are an innovative way to deliver potent cytotoxic compounds to cells expressing a specific antigen, as shown by brentuximab vedotin in patients with CD30-positive lymphomas and for ado-trastuzumab emtansine in patients with HER2-positive breast cancer.4 Another plasma membrane target, CD19, is almost exclusively expressed on B cells, appearing at the pre-B-cell stage and remaining expressed until their terminal differentiation into plasma cells6 and unlike CD20, CD19 is rapidly internalized, which makes it better suited for ADC development.86 Four ADC targeting CD19, SAR3419 (coltuximab ravtansine, huB4-DM4),139 SGN-CD19A (denintuzumab mafodotin),1514 ADCT-402 (loncastuximab tesirine),1816 and SGN-CD19B19 have entered clinical development. SAR3419 is composed of the maytansinoid payload DM4 attached to the anti-CD19 humanized monoclonal IgG1 antibody, huB4, via an N-succinimidyl-4-(2-pyridyldithio)butyrate (SPDB) cleavable linker.9 SAR3419 demonstrated clinical activity in patients with relapsed/refractory B-cell lymphoma as both a single agent and in combination with rituximab.1310 To further, improve the clinical benefit of targeting CD19, second generation ADC can be developed incorporating new innovative linkers and payload moieties.20 For example, the addition of a sulfonate group in the disulfide linker SPDB ([N-succinimidyl 2-sulfo-4-(2-pyridyldithio)butanoate], sulfo-SPDB) can increase the potency of ADC bearing similar payloads.21 The indolinobenzodiazepine pseudodimer DGN462 is a potent DNA-alkylating agent with proven anti-tumor activity in preclinical models of solid tumors and acute myeloid leukemia (AML).22 These two innovations have been implemented in the recently developed IMGN779, an anti-CD33 ADC with promising preclinical2423 and early clinical activity25 in AML. Here, we present huB4-DGN462, a novel ADC utilizing the huB4, anti-CD19 antibody, linked via sulfo-SPDB to DGN462. The huB4-ADC incorporating sulfo-SPDB and DGN462 demonstrated improved in vitro and in vivo activity in lymphoma and leukemia models.

Methods

Cell lines

Lymphoma cell lines, all validated for their identity by short tandem repeat DNA fingerprinting (IDEXX BioResearch, Ludwigsburg, Germany), were used and cultured as previously described.26 BCL2, MYC and TP53 status were defined as reported in Online Supplementary Table S1. Additional cell lines used included the Burkitt’s lymphoma Ramos (ACC-603; DSMZ), and the human acute lymphoblastic leukemia (ALL) cell lines RS4;11 (CRL-1873, ATCC), TOM-1 (ACC-578, DSMZ), and BALL-1 (JCRB0071, JCRB).

Antibody-drug conjugates and free payload

Antibody-drug conjugates (SAR3419, huB4-DGN462, non-targeting IgG-DGN462 control) and cell-permeable unconjugated toxin S-Methyl-DGN462 (DGN462-SMe) were generated by ImmunoGen. huB4-DGN462 and IgG-DGN462 were produced as described previously.22 Briefly, a 10-fold molar excess of in situ mixture containing DGN462 and sulfo-SPDB was added to huB4 or IgG antibody in buffer (50 mM EPPS [4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid], pH 8.5) containing 15% dimethylacetamide. Upon completion of conjugation, the reaction mixtures were purified and buffer exchanged into 20 mM histidine, 50 mM sodium chloride, 8.5% w/v sucrose, 0.01% Tween-20, 50 μM sodium bisulfite pH 6.2 using NAP desalting columns (Illustra Sephadex G-25, GE Healthcare).

In vitro anti-tumor activity

To determine in vitro cytotoxicity potency, B-cell lymphoma and B-ALL cell lines were treated with a 3-fold dilution series of conjugate for five days without or with a 100-fold concentration of unconjugated huB4 blocking antibody. The relative number of viable cells in each well was then determined using the WST-8 based Cell Counting Kit-8 (Dojindo Molecular Technologies Inc., Rockville, MD, USA). The surviving fraction of cells was plotted against conjugate concentration and the EC50 of activity was calculated using a non-linear regression analysis (GraphPad Prims 4.0). For higher-throughput in vitro cytotoxicity screening, lymphoma or leukemia cells (10) were seeded in 96-well plates and the indicated compounds were added to create 4-fold dilution series ranging from 200 nM to 0.19 pM and assayed by MTT following 72 hours (h) of treatment, as previously described.24

Apoptosis was assessed using the luminescence-base Caspase-Glo 3/7 assay kit (Promega) according to the manufacturer’s instructions. Briefly, cells were seeded in 384-well plates, treated with ADC or free toxin at the following concentrations (50 and 1,000 pM for huB4-DGN462 and DGN462-SMe; 100 and 5,000 pM for SAR3419). Apoptosis was defined by at least a 1.5-fold increase in signal activation with respect to controls. Differences among groups were calculated using the Wilcoxon rank-sum test (Stata/SE 12.1 for Mac, Stata Corporation, College Station, TX, USA). P<0.05 was considered statistically significant.

CD19 expression

Fluorescence-activated cell sorting (FACS) for CD19 antigen expression analysis was performed on fresh low passage lymphoma cell lines. Cells were washed with ice-cold FACS buffer (PBS + 0.5% BSA) and divided 1×10 cells/tube. A pretreatment with human FcR blocking (Miltenyi Biotec Inc., Auburn, CA, USA) was performed according to the manufacturer’s instructions. CD19-PE (5 μL; 1μg) or control isotype was incubated with cells at 4°C for 30 minutes. Cells were washed twice and re-suspended in FACS buffer. Flow-cytometry analysis was carried out with a FACS Canto II instrument (BD Biosciences). Median Fluorescence intensity (MFI) of each sample was determined using FacsDiva v.8.0.1 software (BD Biosciences, Allschwil, Switzerland). Unstained cells and cells stained with isotype control antibody were used as controls. RNA expression levels, obtained with the HumanHT-12 v.4 Expression BeadChip (Illumina, San Diego, CA, USA) and with the HTG EdgeSeq Oncology Biomarker panel (HTG Molecular Diagnostics Inc., Tucson, AZ, USA) were published previously.26 For leukemia cell lines, the median number of CD19 antibody binding sites (ABC) was determined as previously described.24 Pairwise correlations were assessed using Stata/SE 12.1 for Mac. P<0.05 was considered statistically significant.

In vivo experiments

Experiments using subcutaneous (DOHH2) and disseminated (Farage) xenograft models in female CB.17 severe combined immunodeficient (SCID) mice (Charles River Laboratories, Wilmington, MA, USA) were run following previously described procedures.27 All animal procedures were performed in strict accordance with Immunogen’s Animal Care and Use Committee and the National Institutes of Health Guide for the Care and Use of Laboratory Animals. For the DOHH2 model, mice were inoculated subcutaneously with 10 cells and were randomized by tumor volume (TV) into treatment groups (6 mice/group), when the average TV reached 100 mm. Tumor volumes were recorded 2-3 times weekly by caliper measurements of the height (H), length (L), and width (W) of the tumor, following the formula:

For the disseminated model, mice were inoculated intravenously (IV) via the lateral tail vein with 10 Farage cells and were randomized into treatment groups (8 mice/group) by body weight. Animal survival was followed; animals were removed from the study when advanced health distress signs were observed or measured (> 20% loss of body weight, hind leg paralysis, tumor growth on body, or moribund state).

Results

huB4-DGN462 is a novel anti-CD19 antibody-drug conjugate

The humanized anti-CD19 antibody huB428 was conjugated with DGN462, a novel member of the DNA-alkylating indolinobenzodiazepine pseudodimers (IGNs) class22 via the cleavable disulfide linker, sulfo-SPDB, at a drug-to-antibody ratio (DAR) of 2.8 (Figure 1A). This novel CD19-targeting ADC, huB4-sulfo-SPDB-DGN462 or huB4- DGN462, combines the anti-CD19 antibody used in SAR3419 (Figure 1B)9 and the potent linker/payload exploited in the anti-CD33 IMGN779.24 To evaluate the consequence of conjugation on antigen binding, the relative binding affinity of huB4-DGN462 and the unconjugated huB4 antibody to CD19 was determined by FACS analysis on the Burkitt’s lymphoma Ramos cell line, which endogenously express human CD19 (Figure 1C). The huB4-DGN462 bound with similar high affinity (KD ~0.15 nM) as the unconjugated antibody, suggesting that the incorporation of the payload did not appreciably alter antibody binding affinity.

Figure 1.huB4-DGN462 is a novel anti-CD19 antibody-drug conjugate (ADC). (A) Chemical structure of huB4-DGN462. (B) Chemical structure of SAR3419. (C) Relative binding affinities of huB4 and huB4-DGN462 on Ramous cells. Cells were cultured with the indicated concentrations of either the unconjugated antibody or intact ADC, and binding was detected by flow cytometry using a fluorescently labeled anti-human antibody. (D) In vitro potency of huB4-DGN462 and SAR3419 in diffuse large B-cell lymphoma (DLBCL) (top) or B-cell acute lymphoblastic leukemia (B-ALL) (bottom) cell lines with or without blocking antibody. ABC: antibodies bound per cell.

huB4-DGN462 has improved in vitro anti-lymphoma activity compared to SAR3419

To characterize the ability of huB4-DGN462 to kill tumor cells, the in vitro cytotoxicity of huB4-DGN462 was compared to SAR3419 in a subset of B-cell lymphoma and B-ALL tumor cell lines. The tumor cell killing was assayed five days after treatment. While SAR3419 had limited potency, huB4-DGN462 was highly active with IC50s ranging from 1-16 pM (Figure 1D). In addition, unconjugated antibody was able to block the activity of both huB4-DGN462 and SAR3419 confirming that the cytotoxicity of both conjugates is the result of specific CD19 antigen binding on tumor cells.

To further characterize the in vitro potency of huB4-DGN462, we performed a 72-h in vitro cytotoxicity screen on 46 B-cell lymphoma cell lines, mostly derived from DLBCL, exposed also to SAR3419 (Table 1 and Online Supplementary Table S1). huB4-DGN462 was potent with a median IC50 of 100 pM (95%CI: 38-214). The free payload, DGN462-SMe, had a median IC50 of 26 pM (95%CI: 1-186) (Online Supplementary Table S1). huB4-DGN462 induced caspase 3/7 activation consistent with an apoptotic mechanism of action (Online Supplementary Table S1). In agreement, cell cycle analysis in two DLBCL cell lines showed a G2-M arrest with subG0 accumulation of cells after huB4-DGN462 and DGN462-SMe treatment (Online Supplementary Figure S1), similar to that reported for IMGN779, a DGN462-containing ADC.24

Table 1.Anti-tumor activity of huB4-DGN462 and SAR3419 in B-cell lymphoma cell lines.

While the rank order of anti-proliferative activity for huB4-DGN462 and SAR3419 was similar across the cell lines tested (R=0.42, P=0.004) (Online Supplementary Figure S2), huB4-DGN462 was over 30-times more potent than SAR3419 (P<0.0001) (Table 1 and Online Supplementary Figure S3). Increased cytotoxic activity of huB4-DGN462 was also confirmed in six CD19 B-ALL cell lines (Online Supplementary Table S2).

While a moderate correlation was seen for SAR3419, the response of B-cell lymphoma lines to huB4-DGN462 was not correlated with either surface CD19 protein expression (as measured by FACS) or CD19 RNA expression (as measured by microarray or by targeted RNA-Seq) (Online Supplementary Figures S4 and S5). However, the activity of huB4-DGN462 was positively correlated with the potency of its free payload (R = 0.71, P<0.0001).

Among DLBCL cell lines, the sensitivity to the two ADCs was not affected by TP53 inactivation (present in 16 of 23 cell lines), nor the presence of MYC or BCL2 translocations (detected in 8 of 24 and 13 of 21 cell lines, respectively). The sensitivity to SAR3419, but not the novel ADC, was lower in activated B-cell-like (ABC) than in germinal center B-cell type (GCB) DLBCL cell lines (P=0.02) (Table 1, Online Supplementary Table S1 and Online Supplementary Figure S6A) This was in agreement with the correlation between SAR3419 activity and CD19 expression and an observed higher CD19 expression in GCB than ABC DLBCL cell lines (Online Supplementary Figure S6B).

huB4-DGN462 has superior in vivo anti-lymphoma activity compared to SAR3419

To examine whether the enhanced antitumor activity of huB4-DGN462 observed in vitro would translate to improved efficacy in vivo, the anti-tumor activity of both of CD19-targeting ADC was evaluated in two DLBCL xenograft models. In the subcutaneous DoHH2 model, a single intravenous dose of huB4-DGN462 resulted in a significant, dose-dependent tumor growth delay and survival benefit at 1.7 mg antibody (Ab)/kg (5 of 6 partial responses, 3 of 6 complete responses, 3 of 6 tumor-free survivors) compared to a non-targeted control IgG-DGN462 ADC (1 of 6 partial responses, 1 of 6 complete responses, 1 of 6 tumor-free survivors) (Figure 2A). The anti-tumor activity of huB4-DGN462 was better than that obtained in a similar experiment using SAR3419 given at higher doses. Both huB4-DGN462 and SAR3419 were well-tolerated, with no significant loss of body weight observed (Online Supplementary Figure S7). In the disseminated Farage model, the 1.7 mg/kg dose of the non-specific IgG1-DGN462 control conjugate was inactive while there was a significant dose-dependent increase in survival observed in mice treated with as low as 0.17 mg Ab/kg of huB4-DGN462 (Figure 2B). At 1.7 mg Ab/kg, survival of tumor bearing mice was increased over 400%. As above, the efficacy of huB4-DGN462 was better than that achieved with SAR3419. Taken together and consistent with the in vitro findings, treatment with huB4-DGN462 resulted in a substantial enhancement of anti-tumor activity compared to SAR3419.

Figure 2.huB4-DGN462 exhibits more potent in situ antitumor efficacy than SAR3419 in both disseminated (A) and subcutaneous (B) germinal center B-cell type diffuse large B-cell lymphoma (GCB DLBCL) xenograft models. (A) Nude mice bearing Farage xenografts were treated with vehicle, a single dose of huB4-DGN462 (0.17, 0.56 or 1.7 mg antibody/kg), or a single dose of non-targeting IgG1-DGN462 (1.7 mg antibody/kg), as indicated. (Right) Nude mice bearing Farage xenografts were treated with vehicle or a single dose of SAR3419 (2.5 or 5 mg antibody/kg) as indicated. The table summarizes the data for the experiments. (B) Nude mice with subcutaneous DOHH2 xenografts were treated with vehicle, a single dose of huB4-DGN462 (0.56 or 1.7 mg antibody/kg), or a single dose of non-targeting IgG1-DGN462 (1.7 mg antibody/kg), as indicated. (Right) Nude mice bearing DOHH2 xenografts were treated with vehicle or a single dose of SAR3419 (2.5 or 5 mg antibody/kg) as indicated. The table summarizes the data for the experiments. Ab: antibody. T/C: treated versus control. PR: partial response (defined when the tumor volume at any given measurement point was <50% of the initial pretreatment tumor volume).²⁷ CR: complete response (defined when no palpable tumor could be detected).²⁷ TGD: tumor growth delay.

Discussion

Here we have compared the anti-lymphoma activity of a novel CD19-targeting ADC, huB4-DGN462, with SAR3419, an ADC that demonstrated clinical activity as single agent2910 and in combination with the anti-CD20 monoclonal antibody rituximab.13 SAR3419 comprises the maytansinoid microtubule disruptor, DM4, conjugated to the huB4 anti-CD19 antibody via the hydrophobic linker SPDB.96 Utilizing the same anti-CD19 antibody in SAR3419, we created huB4-DGN462, which incorporates the ultra-potent DNA-alkylating payload, DGN462 linked by the more hydrophilic sulfo-SPDB linker. This sulfo-SPDB-DGN462 linker/payload combination has previously been shown to have significantly greater potency (up to 3 logs) than the SPDB-DM4.22 As predicted, huB4-DGN462 was more potent than SAR3419 in in vitro anti-proliferative and apoptotic assays as well as in in vivo DLBCL subcutaneous and systemic tumor models. The improved activity of huB4-DGN462 compared to SAR3419 can be attributed to the increased potency of the linker/payload combination of sulfo-SPDB-DGN462 compared to SPDB-DM4.

HuB4-DGN462 was highly active in multiple cell lines derived from B-cell lymphomas. The antitumor activity was not affected by the lymphoma histology or DLBCL subtype or TP53, BCL2 or MYC gene status. Despite specificity for CD19, similar to other ADC, the anti-lymphoma activity of huB4-DGN462 did not correlate with the absolute expression level of CD19,3130 but rather the sensitivity of the cell line to the free payload. The impressive potency of huB4-DGN462 observed is likely to be due to the fact that low target expression was sufficient to deliver a cytotoxic concentration of the novel linker/payload combination. Given the broad expression profile of CD19 in B-cell malignancies, CD19 is an attractive target for cancer therapy. In particular, CD19-targeting immunotherapies have seen recent successes with the US Food and Drug Administration (FDA) approvals of the bispecific T-cell engaging antibody blinatumomab (Blincyto)3432 and the FDA approvals of two chimeric antigen receptors (CAR) T-cell therapies targeting CD19, tisagenlecleucel (Kymriah)35 and axicabtagene cilolecucel (Yescarta).36 Despite the success of CD19-targeting immunotherapies, there still remains the need for more effective therapies, particularly in patients with relapsed/refractory lymphomas such as DLBCL. The clinical activity of CD19-targeting ADC following other anti-CD19 directed therapeutic modalities3932 will have to be determined. However, unlike immunotherapies that stimulate the patient’s immune systems to fight cancer, ADC like huB4-DGN462 kill tumor cells by delivering a cytotoxic payload directly to tumor cells. Given the distinct difference in mechanism of action of ADC to that of immunotherapies, patients have the potential to benefit from both types of therapies.

This work demonstrates that huB4-DGN462 has superior preclinical antitumor activity compared to clinically-validated SAR3419. Each of the individual components of huB4-DGN462 (antibody, linker and payload) have passed early clinical evaluation2513 making huB4-DGN462 an attractive candidate for clinical investigation with the potential to extend the lives of patients with B-cell malignancies.

Footnotes

• ↵* These authors contributed equally to this work.
• Check the online version for the most updated information on this article, online supplements, and information on authorship & disclosures: www.haematologica.org/content/104/8/1633
• FundingPartially supported by institutional research funds from ImmunoGen and the Gelu Foundation (to FB).

• Received November 3, 2018.
• Accepted February 7, 2019.