Sphingosine kinases (SKs) have received the most attention as important enzymes in cancer biology. They participate in the regulation of bioactive sphingolipid metabolism by producing sphingosine-1 phosphate (S1P) which mediates several biological functions, including cell growth, differentiation, cell survival, migration, and angiogenesis among other tasks.1 S1P generation depends on the conversion of sphingosine to S1P, in a reaction catalyzed by two isoforms of SKs, SK1 and SK2, and its levels are tightly controlled via a rapid degradation by intracellular S1P lyases (S1PL) or dephosphorylated by S1P phosphatases.1 Once produced, S1P may function as an intracellular second messenger and/or can be exported outside the cells, where it binds to specific S1P receptors (S1PRs) and initiates downstream signaling pathways, in a paracrine or autocrine manner, in a process known as “inside-out” signaling.1
Chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder characterized by the accumulation of clonal B lymphocytes in peripheral blood and lymphoid tissues. Given that several studies have implicated the SKs/S1P/S1PL pathway as an essential regulator of cell proliferation and survival in cancer cells,42 we evaluated the role of SKs and S1PL in leukemic cells from CLL patients. To this aim we first measured the basal expression of SKs messenger ribonucleic acids (mRNAs) and S1PL mRNA by quantitative real-time polymerase chain reaction (qRT-PCR) on purified B cells from CLL patients and age-matched healthy donors. We found that CLL cells expressed heterogeneous levels of SK1, SK2 and S1PL mRNA, while only SK1 mRNA was statistically significantly higher compared to healthy donors (Figure 1A). As we observed at mRNA level, when SK1 was evaluated by western blot we found a higher expression of the protein in B cells from CLL samples compared to healthy donors (Figure 1B and Online Supplementary Figure S1A). In addition, within CLL patients, there was a positive correlation between SK1 mRNA and protein expression (Online Supplementary Figure S1B). We also observed that the ratio between SK1 and S1PL was increased for B cells from CLL patients compared to B cells from healthy donors (Figure 1C), while no statistically significant differences were found for SK2/S1PL ratios (Online Supplementary Figure S1C). Remarkably, CLL patients with higher SK1/S1PL ratios preferentially belonged to Binet B or C clinical stages, were unmutated, CD38, CD49d and showed a progressive disease (Table 1 and Online Supplementary Table S1), suggesting that SK1/S1PL molecules might participate in the clinical outcome of the patients, favoring the progression of the disease.
Malignant B cells from CLL patients mainly receive advantageous signals in lymphoid tissues, where the supportive microenvironment and B cell receptor (BCR) signaling promote their activation and proliferation, modulate cell adhesion and migration and protect CLL cells from spontaneous and drug-induced apoptosis.5 We have previously reported that CLL cells activated by signals from the supportive microenvironment transiently impair the expression of S1PR1,6 in a process that may extend the residency of the leukemic clone within the survival niches of lymphoid tissues. In the study herein we wanted to determine whether the activation of CLL cells has any influence on SK1/S1PL ratios. To this aim, purified B cells from CLL patients were cultured in the presence of immobilized Fab′ 2 anti-human immunoglobulin M (anti-IgM) and CD40L, CpG or HS5 cell line as a feeder layer and, as expected, after 24 hours of culture, the stimuli induced the upregulation of the activation marker CD69 on CLL cells (data not shown). Interestingly, we found that CLL activation enhanced SK1/S1PL ratios in all of the patients evaluated (Figure 1D), independently of their clinical stage or the prognosis group (data not shown). Ibrutinib, by impairing leukemic cell activation (data not shown) was able to strongly reduce the upregulation of SK1/S1PL ratios induced by the stimulus (Online Supplementary Figure S2). Moreover, in order to evaluate the expression of SK1/S1PL ratios within in vivo activated CLL cell subpopulations, we took advantage of the fact that we had previously obtained mRNA samples of in vivo activated CLL cells subpopulations and non-activated counterparts of the same patient.6 When SK1, SK2 and S1PL mRNA levels were evaluated in the proliferative fraction of circulating CLL cells (PF, IgG cells), described by Palacios et al.,7 and the quiescent fraction from the same patient (IgMIgG cells, QF), we found higher SK1/S1PL ratios in the PF of the three CLL patients evaluated (Figure 1E). Additionally, bone marrow leukemic cells expressing high levels of CD38, which defines a subpopulation of activated lymphocytes, showed higher SK1/S1PL ratios compared to their CD38 low or negative counterparts (n=3) (Figure 1F), indicating that in vivo activated CLL cells expressed higher ratios of SK1/S1PL compared with the rest of the leukemic clone.
Subsequently, to test whether the inhibition of SKs could modify the survival of CLL cells, we employed SKI-II, which is an orally bioavailable inhibitor for SK1 and SK2 that blocks S1P production and cell proliferation.8 When peripheral blood mononuclear cells (PBMCs) from CLL patients were cultured with different doses of SKI-II, we found that SKI-II induced cell death in a dose-dependent way (Figure 2A and Online Supplementary Figure S3A). We observed that the percentage of cell death induced by 50 μM of SKI-II inversely correlated with the basal SK1/S1PL ratios of each sample (Online Supplementary Figure S3B). In line with this, CLL patients with low SK1/S1PL ratios were more sensitive to 50 μM of SKI-II compared to patients with high SK1/S1PL ratios (Figure 2B). In addition, we found that non-apoptotic doses of SKI-II (15 μM) increased the susceptibility of CLL cells to die by other therapeutic agents, such as fludarabine, bendamustine and ibrutinib (Figure 2C), more markedly in CLL patients with low SK1/S1PL ratios compared to CLL patients with high SK1/S1PL ratios (Online Supplementary Figure S3C). Similar results were recently reported by others showing that SKs inhibition induces apoptosis in primary multiple myeloma,9 acute myeloid leukemia10 and natural killer-large granular lymphocyte leukemia cells11 and also sensitizes CLL cells to other therapeutic agents such as EGCG (-epigallocatechin-O-3-gallate)12 or lysosome-targeting drugs.13 SKI-II-induced cell death was associated with a downregulation of BCL-2 expression in CLL cells (Online Supplementary Figure S3D), as previously reported in SKI-II-treated gastric cancer cells.14 Thus, CLL samples with low SK1/S1PL ratios, which were more sensitive to 50 μM of SKI-II (Figure 2B), showed a higher BCL-2 downregulation upon SKI-II treatment compared to CLL patients with high SK1/S1PL ratios (Figure 2D and Online Supplementary Figure S3E).
On the other hand, we found that non-apoptotic doses of SKI-II were able to impair CLL activation induced by anti-IgM + CD40L at 24 hours, measured by both the upregulation of CD69 (Figure 2E) and CLL proliferation evaluated at 5 days (Figure 2F). Remarkably, those samples with high SK1/S1PL ratios presented a greater leukemic cell proliferation induced by anti-IgM + CD40L compared to patients with low SK1/S1PL (Figure 2F), suggesting that the SK1/S1P/S1PL pathway might regulate this proliferative response in CLL. Representative carboxyfluorescein succinimidyl ester (CFSE) histograms are depicted in the Online Supplementary Figure S3F.
Finally, in order to determine whether S1PRs signaling may counteract the effects of SKI-II, we added exogenous S1P to the cultures and found that it rescued CLL cells from the cell death induced by 50 μM of SKI-II (Figure 2G) and from the anti-proliferative effect of 15 μM of SKI-II (Figure 2H). These findings suggest that S1P production by CLL cells may favor leukemic cell survival and proliferation in an “inside-out” signaling manner acting through S1PRs.1
Taken together, the results presented herein and our previous data showing that the activation of CLL cells transiently impair the expression of S1PR1,6 allow us to hypothesize about the role of the SK1/S1P/S1PL axis in CLL. Thus, the possibility exists that microenvironment signals from lymphoid tissues, by increasing SK1/S1PL ratios in leukemic CLL cells, favors S1P production. Exported S1P can then bind S1PRs, reduce S1PR1 expression6 and transduce stimulating signals to the leukemic clone.
In conclusion, our results suggest that the SK/S1P/S1PL pathway supports the survival and proliferation of CLL cells, hence favoring the progression of the disease, thus we encourage the use of SKs inhibitors in combined therapy as a promising treatment option in the future.
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