Abstract
Acute myeloid leukemia carrying NPM1 mutations and cytoplasmic nucleophosmin (NPMc+ acute myeloid leukemia) represents one-third of adult AML (50–60% of all acute myeloid leukemia with normal karyotype) and shows distinct biological, pathological and clinical features. We confirm in 2562 patients with acute myeloid leukemia our previous observation that NPM1 mutations and cytoplasmic nucleophosmin are mutually exclusive of recurrent genetic abnormalities. Taken together, these findings make NPMc+ acute myeloid leukemia a good candidate for inclusion in the upcoming World Health Organization classification.Introduction
One of the most controversial issues in the WHO classification of myeloid malignancies is acute myeloid leukemias not otherwise characterized.1 This category accounts for 60–70% of AML, including cases with normal cytogenetics (AML-NC) which are characterized by a great molecular, pathological and clinical heterogeneity. AML not otherwise characterized is currently defined according to FAB criteria which are, however, insufficient to characterize AML-NC, since they do not identify distinct disease entities within this heterogeneous group. Recognition of underlying molecular alterations is crucial for the improvement of AML-NC classification.
We previously identified nucleophosmin (NPM1) mutations, causing aberrant cytoplasmic expression of NPM,2–4 as the most frequent genetic alteration associated with AML-NC (50–60% of cases). NPM1 mutations and cytoplasmic NPM appear to be mutually exclusive of recurrent genetic abnormalities2 and identify a leukemia subgroup called NPMc+ (cytoplasmic-positive) AML that show distinct biological, pathological and clinical features,5,6 as well as a unique gene expression profile.7 For these reasons, NPMc+ AML (as well as AML with CEBPA mutations that also appear to be mutually exclusive with recurrent genetic abnormalities) is a good candidate for inclusion as a new entity in the forthcoming WHO classification. However, since a few studies8–11 have reported very rare cases of NPM1 mutations coinciding with either inv(16), t(8;21) or t(9;22), we extended our analysis to 2562 patients with acute myeloid leukemia.
Design and Methods
Leukemic samples
The aim of the study for AML patients enrolled in the GIMEMA LAM99P and the GIMEMA/EORTC AML12 trials was to compare the subcellular expression of NPM with the results of molecular studies for recurrent genetic alterations. The rationale for this approach derives from our previous observations that immunohistochemistry is fully predictive of NPM1 mutation status.12 Bone marrow biopsies for immunohistochemical studies were available from 1073 unselected, consecutively observed AML cases, 591 of whom had been previously reported.2 Ninety-six acute promyelocytic leukemia cases (not included in the GIMEMA LAM 99P and GIMEMA/EORCT-AML12) were also available for comparative immunohistochemical and molecular analysis. In addition, 1403 unselected AML cases were investigated at the Munich Leukemia Laboratory (MLL). Cytogenetics failed in 10 cases and these were excluded from the present study. Therefore, the Munich cohort consisted of 1393 consecutively observed, unselected newly diagnosed AML for which complete cytogenetic/molecular studies were available. In these cases, we compared the results of NPM1 mutational analysis13 and cytogenetic/molecular studies. Written informed consents for the study was obtained from each participating center.
Immunohistochemical studies
Immunohistochemistry for NPM was available in 1073 AML cases, 591 of whom had been previously reported.2 NPM subcellular expression was detected in paraffin-sections from B5-fixed/EDTA decalcified bone marrow trephines using a specific anti-NPM monoclonal antibody (clone 376) and the highly sensitive alkaline phosphatase anti-alkaline phosphatase (APAAP) technique, as previously described.2 Monoclonal antibody against nucleolin/C23 was purchased from Santa Cruz Biotechnology.
Cytogenetics and molecular studies for recurrent genetic abnormalities
Cytogenetic investigation was performed after short-term culture. Karyotypes were analyzed after G-banding and described according to the International System for Human Cytogenetic Nomenclature.14 Fluorescence in situ hybridization was carried out according to standard techniques. Reverse-transcriptase-polymerase-chain-reaction (RT-PCR) for AML1-ETO, CBFB-MYH11, DEK-CAN and BCR-ABL, performed as previously reported,2 was available in 1019/1073, 1020/1073, 936/1073 and 1015/1073 AML cases respectively. Southern blotting and FISH for rearrangements of the mixed-lineage leukemia gene (MLL)2 was carried out in 728/1073 patients.
Mutational analysis of the NPM1 gene
Mononucleated cells were isolated by standard Ficoll-Hypaque density gradient centrifugation. Nucleic acid isolation, cDNA synthesis and screening for NPM1 gene mutations were performed using a melting curve based LightCycler assay, as previously described.13 AML samples with an aberrant melting curve underwent nucleotide sequence analysis.
Results and Discussion
Of the 1073 bone marrow biopsies from patients of the GIMEMA LAM99P and GIMEMA/EORTC AML12 trials, 368 (34.2%) were characterized by aberrant cytoplasmic expression of nucleophosmin (NPMc+) associated with nucleus-restricted positivity for nucleolin (C23) (Figure 1). As previously reported,12 this staining pattern is fully predictive of a mutated NPM1 gene. All other biopsies showed a nucleus-restricted positivity for nucleophosmin (NPMc-) which is fully predictive of NPM1 gene in a germline configuration.12 Comparison of immunohistochemistry with the results of molecular studies for the recurrent genetic abnormalities is shown in Table 1. Remarkably, all 300 AMLs carrying various types of recurrent genetic abnormalities showed a nucleus-restricted expression of NPM. Representative examples of these staining patterns in AML carrying the most frequent recurrent genetic abnormalities, i.e. t(15;17), inv(16) and t(8;21), are shown in Figure 2. Completely overlapping results were obtained in cases from the Munich Leukemia Laboratory, by comparing the mutation status of the NPM1 gene with the results on cytogenetic/molecular studies for recurrent genetic abnormalities. In total, we investigated 1393 AML cases, 835 of which had a normal and 558 an abnormal karyotype. NPM1 mutations were detected in 420 out of 1393 patients (30.1%) while the other cases harboured a NPM1 gene in a germline configuration. In 246 of 1393 patients, reciprocal translocations and the respective fusion genes were detected; RT-PCR and/or FISH were performed for all respective fusion genes. The results are shown in Table 2. Notably, none of the 246 AMLs carrying different types of genetic abnormalities harbored NPM1 mutations. These results were obtained independently from a total of 2562 AMLs from two large series using different approaches, support our original finding that, cytoplasmic mutated NPM is mutually exclusive of recurrent genetic abnormalities.2 Our findings raise several issues surrounding the significance of the sporadic cases of NPM1 mutations coinciding with recurrent genetic abnormalities, as reported in some studies.8–10 First, none of these studies documented whether NPM1 mutations and concurrent genetic abnormalities occur in the same or different leukemic cell populations. Another major concern is specificity control in large multi-center trials. This is addressed in Falini B. et al.2 (Supplementary Materials). In particular, 4 patients in the GIMEMA EORTC AML12 trial were diagnosed as having cytoplasmic mutated NPM together with inv(16), t(8;21) or MLL rearrangement. In-depth re-investigation of these cases revealed errors in sample registration or PCR contamination. Because of the presence of NPM1 mutation in association with a t(16;16)/MYH11-CBFB transcript, 1 patient from the CCG-2961 AML pediatric trial was also re-investigated and found not to have a NPM1 mutation (Patrick Brown, unpublished results). Therefore, in our experience, re-analysis according to strict criteria showed that NPM1 mutations are mutually exclusive of recurrent genetic abnormalities. Whether the occurrence of two or more specific genetic markers in exceptional cases is just coincidental or represents a true association is still not understood. Rare examples include t(15;17) plus t(8;21),15 t(15;17) plus t(9;22),16 and t(9;22) or BCR-ABL plus inv(16).17,18 Interestingly, 1 case reported by Thiede et al.9 carried both a t(8;21) and an inv(16) in addition to NPM1 mutations with a NPM1 mut/wt ratio of only 0.37 (Christian Thiede, personal communication on February 6, 2006). More recently, coexistence of JAK2V617F and BCR-ABL has also been reported in chronic myeloproliferative disorders19,20 and association of JAK2V617F with t(8;21) in AML therapy-related21 or secondary to a myeloproliferative syndrome.22 In conclusion, our data unequivocally confirm in a large series of patients with acute myeloid leukemia that NPM1 mutations are mutually exclusive of other recurrent genetic abnormalities and that NPM1 mutations identify a distinct acute myeloid leukemia genetic entity which should be considered for inclusion in the upcoming WHO Classification.
Acknowledgments
we would like to thank Roberta Pacini and Manola Carini for performing the immunohistochemical stainings and Mrs. Claudia Tibidò for her secretarial assistance
Footnotes
- Authorship and Disclosures BF is responsible for the study concept and wrote the manuscript; GS, FLC, DD, FP, CM, MM were involved ni the molecular and cytogenetic studies of patients from GIMEMA/EORTC AML12; MPM and SP were involved in the immunohistochemical study of NPM expression in AML specimens. TH, CH and SS carried out the molecular analysis of cases from the Munich Leukemia Laboratory. BF and CM applied for a patent on the clinical use of NPM1 mutants.
- Funding: This work was supported by the Associazione Italiana per la Ricerca sul Cancro (A.I.R.C.).
- Received August 24, 2007.
- Accepted December 17, 2007.
References
- Vardiman JW, Harris NL, Brunning RD. The World Health Organization (WHO) classification of the myeloid neoplasms. Blood. 2002; 100:2292-302. Google Scholar
- Falini B, Mecucci C, Tiacci E, Alcalay M, Rosati R, Pasqualucci L. Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype. N Engl J Med. 2005; 352:254-66. Google Scholar
- Falini B, Bolli N, Shan J, Martelli MP, Liso A, Pucciarini A. Both carboxy-terminus NES motif and mutated tryptophan(s) are crucial for aberrant nuclear export of nucleophosmin leukemic mutants in NPMc+ AML. Blood. 2006; 107:4514-23. Google Scholar
- Bolli N, Nicoletti I, De Marco MF, Bigerna B, Pucciarini A, Mannucci R. Born to be exported: COOH-terminal nuclear export signals of different strength ensure cytoplasmic accumulation of nucleophosmin leukemic mutants. Cancer Res. 2007; 67:6230-7. Google Scholar
- Falini B, Nicoletti I, Martelli MF, Mecucci C. Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biologic and clinical features. Blood. 2007; 109:874-85. Google Scholar
- Falini B, Nicoletti I, Bolli N, Martelli MP, Liso A, Gorello P. Translocations and mutations involving the nucleophosmin (NPM1) gene in lymphomas and leukemias. Haematologica. 2007; 92:519-32. Google Scholar
- Alcalay M, Tiacci E, Bergomas R, Bigerna B, Venturini E, Minardi SP. Acute myeloid leukemia bearing cytoplasmic nucleophosmin (NPMc+ AML) shows a distinct gene expression profile characterized by up-regulation of genes involved in stem-cell maintenance. Blood. 2005; 106:899-902. Google Scholar
- Suzuki T, Kiyoi H, Ozeki K, Tomita A, Yamaji S, Suzuki R. Clinical characteristics and prognostic implications of NPM1 mutations in acute myeloid leukemia. Blood. 2005; 106:2854-61. Google Scholar
- Thiede C, Koch S, Creutzig E, Steudel C, Illmer T, Schaich M. Prevalence and prognostic impact of NPM1 mutations in 1485 adult patients with acute myeloid leukemia (AML). Blood. 2006; 107:4011-20. Google Scholar
- Verhaak RG, Goudswaard CS, van Putten W, Bijl MA, Sanders MA, Hugens W. Mutations in nucleophosmin (NPM1) in acute myeloid leukemia (AML): association with other gene abnormalities and previously established gene expression signatures and their favorable prognostic significance. Blood. 2005; 106:3747-54. Google Scholar
- Palmisano M, Grafone T, Ottaviani E, Testoni N, Baccarani M, Martinelli G. NPM1 mutations are more stable than FLT3 mutations during the course of disease in patients with acute myeloid leukemia. Haemato-logica. 2007; 92:1268-9. Google Scholar
- Falini B, Martelli MP, Bolli N, Bonasso R, Ghia E, Pallotta MT. Immunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia. Blood. 2006; 108:1999-2005. Google Scholar
- Schnittger S, Schoch C, Kern W, Mecucci C, Tschulik C, Martelli MF. Nucleophosmin gene mutations are predictors of favorable prognosis in acute myelogenous leukemia with a normal karyotype. Blood. 2005; 106:3733-9. Google Scholar
- Mitelman F. ISCN 1995: an international system for human cytogenetic nomenclature. Switzerland: Basel; 1995. Google Scholar
- Bonomi R, Giordano H, del Pilar Moreno M, Bodega E, Landoni AI, Gallagher R. Simultaneous PML/RARalpha and AML1/ETO expression with t(15;17) at onset and relapse with only t(8;21) in an acute promyelocytic leukemia patient. Cancer Genet Cytogenet. 2000; 123:41-3. Google Scholar
- Scolnik MP, Palacios MF, Acevedo SH, Castuma MV, Larripa IB, Palumbo A. Promyelocytic blast crisis of chronic myelogenous leukaemia with translocations (9;22) and (15;17). Leuk Lymphoma. 1998; 31:231-6. Google Scholar
- Mecucci C, Noens L, Aventin A, Testoni N, Van den Berghe H. Philadelphia-positive acute myelomonocytic leukemia with inversion of chromosome 16 and eosinobasophils. Am J Hematol. 1988; 27:69-71. Google Scholar
- Svaldi M, Lanthaler A, Venturi R, Coser P, Mitterer M. Simultaneous occurrence of bcr-abl and inv16 in a case of M1 acute myeloid leukemia. Leukemia. 2001; 15:695. Google Scholar
- Hussein K, Bock O, Seegers A, Flasshove M, Henneke F, Buesche G. Myelofibrosis evolving during imatinib treatment of a chronic myeloproliferative disease with coexisting BCR-ABL translocation and JAK2V617F mutation. Blood. 2007; 109:4106-7. Google Scholar
- Kramer A, Reiter A, Kruth J, Erben P, Hochhaus A, Muller M. JAK2-V617F mutation in a patient with Philadelphia-chromosome-positive chronic myeloid leukaemia. Lancet Oncol. 2007; 8:658-60. Google Scholar
- Schnittger S, Bacher U, Kern W, Haferlach C, Haferlach T. JAK2 seems to be a typical cooperating mutation in therapy-related t(8;21)/AML1-ETO-positive AML. Leukemia. 2007; 21:183-4. Google Scholar
- Schneider F, Bohlander SK, Schneider S, Papadaki C, Kakadyia P, Dufour A. AML1-ETO meets JAK2: clinical evidence for the two hit model of leukemogenesis from a myeloproliferative syndrome progressing to acute myeloid leukemia. Leukemia. 2007; 21:2199-201. Google Scholar