Efficacy of novel culture environments on the ex vivo expansion kinetics of cord blood progenitor cells

Abstract

We demonstrate the efficacy of two cytokine-rich supernatants, one from peripheral blood cell cultures stimulated with anti-CD3 and the other from monocyte-conditioned media, in enhancing the rapid (within 6 days) expansion and clonogenicity of CD34+, progenitor cells. We, thereby,increased the generation of mostly myeloid precursors able to support stem cell transplants.