A prospective description of HIV-associated multicentric Castleman disease in Malawi

Despite high burden of Kaposi sarcoma (KS) and HIV, multicentric Castleman disease (MCD) is rarely reported in sub-Saharan Africa (SSA). A pathology series in Uganda demonstrated latency-associated nuclear antigen (LANA) and viral interleukin-6 positivity by immunohistochemistry (IHC) in 3 of 64 reactive-appearing lymph nodes, consistent with missed MCD diagnoses,1 but this was before antiretroviral therapy (ART) scale-up when MCD may have been relatively infrequent. In South Africa, a pathology series reported many HIV B-cell lymphoproliferations without specifically mentioning MCD.2 A retrospective series from South Africa reported 35 MCD cases during 1990–2014, with most cases in the most recent years within this time period.3

Malawi is a low-income country in SSA with 9% HIV prevalence and 66% ART coverage.4 HIV malignancies are common and KS accounts for one-third of all cancers.5 However, the first MCD cases were reported by our group in 2014-2015.76 This paper supplements earlier reports by describing a larger cohort of MCD patients with longer follow up, diagnosed and treated under real-world conditions in Malawi. To our knowledge, this is the first prospective cohort description of HIV MCD from SSA, where KS-associated herpesvirus (KSHV) and HIV are endemic.

Kamuzu Central Hospital (KCH) is the cancer referral hospital for one-half of the Malawi population (population: approx. 18 million). The KCH Lymphoma Study (clinicaltrials.gov identifier: 02835911) is a prospective observational cohort initiated in 2013 to characterize the clinicopathological spectrum of lymphoproliferative disorders in this environment in patients with and without HIV. Study procedures have been described previously.8 The study was approved by the Malawi National Health Sciences Research Committee and the University of North Carolina Biomedical Institutional Review Board, USA. Briefly, through active case finding across all hospital departments and referring clinics, adults and children with newly diagnosed lymphoproliferative disorders were invited to participate after written informed consent.8 Diagnostic evaluation has also been previously described, but includes histological confirmation in real-time with immunohistochemistry (IHC) and weekly telepathology conferences using a virtual microscopy link between Lilongwe, Malawi, and Chapel Hill, USA, involving clinicians and 2-4 Malawian and United States (US) pathologists who reach a consensus diagnosis.9 Adults ≥18 years with confirmed MCD between June 2013 and May 2018 were included in these analyses.

During most of the study, first-line treatment for MCD was etoposide 100 mg/m weekly for eight doses then biweekly for eight doses. Most patients received initial brief 5-7-day courses of steroids before starting etoposide. Second-line treatment was CVP [cyclophosphamide 750 mg/m day 1, vincristine 1.4 mg/m (max 2 mg) day 1, prednisone 60 mg/m days 1-5] every 21 days for 6-8 cycles. This treatment protocol was initiated for several reasons: 1) availability and cost of chemotherapy agents; 2) no public sector availability nor registration for rituximab; 3) scarce data regarding MCD treatment in SSA; 4) desire to include a drug with KS activity to treat clinical or subclinical KS; 5) concern about multiagent chemotherapy in patients with markedly impaired performance status; 6) concern about steroids exacerbating clinical or subclinical KS; 7) use of rituximab with etoposide as a common MCD treatment in high-income countries; and 8) presence of hemophagocytic lymphohistiocytosis (HLH) in at least one case. More recently, first-line treatment was changed to CVP due to seemingly longer response durations.

After August 2016, based on frequent relapses after chemotherapy, we leveraged an ongoing phase II trial of rituximab with chemotherapy for diffuse large B-cell lymphoma in Lilongwe, Malawi,10 to petition Malawi regulatory authorities to allow compassionate use exemption for rituximab to treat relapsed MCD. For these patients, rituximab was administered at 375 mg/m weekly for four doses.

Given the lack of laboratory capacity to measure KSHV load or inflammatory markers in real time in Lilongwe, treatment response was defined as completion of therapy (16 etoposide doses, 6-8 CVP cycles, or four rituximab doses) with improved subjective systemic symptoms, improved clinical adenopathy and hepatosplenomegaly, and improved hemogloblin level, as compared with baseline. Patients were followed until death or administrative censoring on 31 May 2018. Kaplan-Meier methods were used to estimate overall survival (OS).

During the study period, 137 HIV adults with confirmed lymphoproliferative disorders were enrolled, of whom 22 (16%) had MCD. All MCD patients presented with lymphadenopathy and MCD diagnosis was based on lymph node biopsy with characteristic morphology and LANA IHC positivity in all cases. An illustrative case is shown in Figure 1. MCD was approximately three times more common than HIV Hodgkin lymphoma (n=7, 5%) during the study period, and no HIV- or KSHV-MCD cases were diagnosed during this period. Baseline characteristics for MCD patients are shown in Table 1. Median age was 41 years (range 27-57), 14 (64%) were male, and 8 (36%) received empiric tuberculosis treatment before MCD diagnosis. Twenty-one (95%) were on ART, with median ART duration 53 months (range 12-179), median CD4 count 306 cells/μL (range 2-1146), and 15 patients (68%) with suppressed HIV loads. Three patients had concurrent KS, and one met diagnostic criteria for HLH. Of eight patients with baseline plasma KSHV loads tested in the US, all were positive with median level 4.1 log₁₀copies/mL (range 2.7-5.2).

Figure 1.Histological images from a lymph node involved by multicentric Castleman disease and concomitant Kaposi sarcoma. Representative histological images are shown from a patient with concomitant (A and B) multicentric Castleman disease (MCD) and (C and D) Kaposi sarcoma (KS). Hematoxylin & eosin stain sections (A and C) are shown along with latency-associated nuclear antigen (LANA) immunohistochemistry (B and D). The MCD lesion is characterized by involuted germinal centers with penetrating hyalinized vessels, and prominent onion skinning of the surrounding mantle zone (A). LANA-positive plasmablasts are highlighted by immunohistochemistry (B). A separate focus of KS exhibits spindled endothelial cells with slit like spaces and numerous extravasated red blood cells (C). The spindled neoplastic cells show nuclear LANA staining (D). Photomicrographs were acquired on an Olympus BX43 microscope with a 20x objective lens (UPlanFL, numerical aperture = 0.50) using an Olympus DP27 camera and Olympus cellSens Standard 1.16 software. Images were sharpened and brightened using Adobe Photoshop Creative Cloud (2017.1.1 Release) without additional manipulation.

Table 1.Baseline characteristics of patients with HIV-associated multicentric Castleman disease in Lilongwe, Malawi.

As of 31 May 2018, disease and vital status were known for all patients. One-year OS was 68% (95%CI; 42-85%) (Figure 2). Two patients died before chemotherapy initiation. Seventeen patients were treated with first-line etoposide, of whom 11 (65%) responded. However, eight responders experienced relapse with median time to death or next treatment of 1.2 months (range 0.2-7.8). Of 10 patients who completed second-line CVP, nine (90%) responded, but seven experienced relapse with a median time to death or next treatment of 3.3 months (range 0.4-11.9). Of these seven, five received rituximab after compassionate use exemption from Malawi regulatory authorities, and four were in remission after median follow up of 10.9 months (range 5.8-14.8). One patient developed KS worsening on rituximab requiring paclitaxel. Of nine deaths in the cohort, seven were from MCD, one from an infectious treatment-related complication, and one unrelated to MCD or treatment.

Figure 2.Overall survival for HIV-associated multicentric Castleman disease in Lilongwe, Malawi.

To our knowledge, this is the first well-characterized prospective cohort of HIV MCD in SSA, a region with high HIV and KSHV burden. MCD occurred typically in patients on long-term ART. Many patients were severely ill on presentation and the mortality rate was high. Recapitulating experience in high-income countries (HICs),11 responses to chemotherapy were short-lived, and rituximab was well-tolerated with longer response durations. Unlike HICs, where MCD is highly heterogeneous with many HIV and KSHV cases,12 we only observed HIV KSHV MCD in our setting, which is not surprising given the high prevalence of both viruses in Malawi.

The infrequent reports of MCD in SSA are probably due to under-diagnosis; IHC is often not performed thus limiting the pathology spectrum.13 MCD can mimic HIV reactive lymphadenopathy and diagnosis is difficult without LANA IHC. In addition, the differential diagnosis of fever and lymphadenopathy in HIV individuals is broad,14 and MCD may be missed where there is little awareness of the disease, as reflected by frequent empiric tuberculosis treatment in our patients.

Once diagnosed, MCD treatment options in SSA are limited. In our experience, chemotherapy produced transient responses with frequent rapid relapses once therapy was discontinued. After the first identification of MCD in Malawi, we were fortunate to have rituximab available for an ongoing phase II trial for diffuse large B-cell lymphoma.10 We were also fortunate to receive what may be, to our knowledge, the first ever compassionate use exemption from Malawi regulatory authorities, in order to treat relapsed MCD with rituximab. In the limited experience to date, rituximab was well-tolerated by patients with high response rates, including responses that appear more durable than those achieved after chemotherapy, even similar to resource-rich settings.11

Our experience demonstrates the value of close collaboration between SSA clinicians and pathologists. In Malawi, between 2014 and 2018, this collaboration led to the first identification of MCD and the development of a staged treatment strategy, despite severely constrained resources, which has improved outcomes compared with our initial reports.7 MCD awareness in Lilongwe has increased, which is important given that MCD may increase with ART scale-up given the data in high-income countries15 and the profile of MCD patients in Malawi who typically had well-controlled HIV on long-term ART. Addressing this emerging problem will require continued investments in laboratory capacity (e.g. pathology, LANA IHC, KSHV load assays) and clinical care, including advocating for greater rituximab availability and trials to assess novel MCD agents.

In conclusion, MCD occurs relatively frequently in Malawi, and unlike HICs, is uniformly associated with HIV and KSHV. MCD presents significant diagnostic and treatment challenges in SSA. Improved awareness, laboratory capacity, and treatments are needed to address this emerging and under-recognized problem, but rapid progress is achievable even in low-income country settings through sustained multidisciplinary collaboration.