TY - JOUR AU - Michaela Nováková, AU - Markéta Žaliová, AU - Martina Suková, AU - Marcin Wlodarski, AU - Aleš Janda, AU - Eva Froňková, AU - Vít Campr, AU - Kateřina Lejhancová, AU - Ondřej Zapletal, AU - Dagmar Pospíšilová, AU - Zdeňka Černá, AU - Tomáš Kuhn, AU - Peter Švec, AU - Vendula Pelková, AU - Zuzana Zemanová, AU - Gitte Kerndrup, AU - Marry van den Heuvel-Eibrink, AU - Vincent van der Velden, AU - Charlotte Niemeyer, AU - Tomáš Kalina, AU - Jan Trka, AU - Jan Starý, AU - Ondřej Hrušák, AU - Ester Mejstříková, PY - 2016/05/31 Y2 - 2024/03/29 TI - Loss of B cells and their precursors is the most constant feature of GATA-2 deficiency in childhood myelodysplastic syndrome JF - Haematologica JA - haematol VL - 101 IS - 6 SE - Articles DO - 10.3324/haematol.2015.137711 UR - https://haematologica.org/article/view/7736 SP - 707-716 AB - GATA-2 deficiency was recently described as common cause of overlapping syndromes of immunodeficiency, lymphedema, familiar myelodysplastic syndrome or acute myeloid leukemia. The aim of our study was to analyze bone marrow and peripheral blood samples of children with myelodysplastic syndrome or aplastic anemia to define prevalence of the GATA2 mutation and to assess whether mutations in GATA-2 transcription factor exhibit specific immunophenotypic features. The prevalence of a GATA2 mutation in a consecutively diagnosed cohort of children was 14% in advanced forms of myelodysplastic syndrome (refractory anemia with excess blasts, refractory anemia with excess blasts in transformation, and myelodysplasia-related acute myeloid leukemia), 17% in refractory cytopenia of childhood, and 0% in aplastic anemia. In GATA-2-deficient cases, we found the most profound B-cell lymphopenia, including its progenitors in blood and bone marrow, which correlated with significantly diminished intronRSS-Kde recombination excision circles in comparison to other myelodysplastic syndrome/aplastic anemia cases. The other typical features of GATA-2 deficiency (monocytopenia and natural killer cell lymphopenia) were less discriminative. In conclusion, we suggest screening for GATA2 mutations in pediatric myelodysplastic syndrome, preferentially in patients with impaired B-cell homeostasis in bone marrow and peripheral blood (low number of progenitors, intronRSS-Kde recombination excision circles and naïve cells). ER -