TY - JOUR AU - Yiren Xiao, AU - Zhiwu Jiang, AU - Yin Li, AU - Wei Ye, AU - Bei Jia, AU - Minjie Zhang, AU - Yan Xu, AU - Donghai Wu, AU - Liangxue Lai, AU - Yaoyu Chen, AU - Yingjun Chang, AU - Xiaojun Huang, AU - Hudan Liu, AU - Guoliang Qing, AU - Pentao Liu, AU - Yangqiu Li, AU - Bing Xu, AU - Mei Zhong, AU - Yao Yao, AU - Duanqing Pei, AU - Peng Li, PY - 2015/05/05 Y2 - 2024/03/29 TI - ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells JF - Haematologica JA - haematol VL - 100 IS - 5 SE - Articles DO - 10.3324/haematol.2014.118612 UR - https://haematologica.org/article/view/7366 SP - 585-594 AB - Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration. ER -