TY - JOUR AU - Giacomo Gianfaldoni, AU - Francesco Mannelli, AU - Vanessa Ponziani, AU - Giovanni Longo, AU - Sara Bencini, AU - Alberto Bosi, AU - Alessandro M. Vannucchi, PY - 2010/05/04 Y2 - 2024/03/29 TI - Early reduction of WT1 transcripts during induction chemotherapy predicts for longer disease free and overall survival in acute myeloid leukemia JF - Haematologica JA - haematol VL - 95 IS - 5 SE - Brief Reports DO - 10.3324/haematol.2009.011908 UR - https://haematologica.org/article/view/5597 SP - 833-836 AB - We investigated the prognostic significance of early peripheral blast clearance as assessed by WT1 transcript reduction during the first days of standard induction therapy in 57 adult patients with acute myeloid leukemia (AML). Quantification of WT1 transcript by real-time quantitative PCR in peripheral blood on days 1 and 5 of treatment was performed. WT1 ratio was defined as the ratio of copy number measured on day 1 and on day 5. The median WT1 ratio was greater in patients attaining CR as compared to non-responders (11.68 vs. 2.14, respectively; P=0.0006). Furthermore, DFS and OS were significantly longer in patients displaying a WT1 ratio greater than 5.82 (i.e. the median value of whole cohort) than in patients with WT1 ratio of 5.82 or under (P=0.024 and P