TY - JOUR AU - Luca Maurillo, AU - Francesco Buccisano, AU - Alessandra Spagnoli, AU - Giovanni Del Poeta, AU - Paola Panetta, AU - Benedetta Neri, AU - Maria Ilaria Del Principe, AU - Carla Mazzone, AU - Maria Irno Consalvo, AU - Anna Tamburini, AU - Licia Ottaviani, AU - Daniela Fraboni, AU - Chiara Sarlo, AU - Paolo De Fabritiis, AU - Sergio Amadori, AU - Adriano Venditti, PY - 2007/05/01 Y2 - 2024/03/29 TI - Monitoring of minimal residual disease in adult acute myeloid leukemia using peripheral blood as an alternative source to bone marrow JF - Haematologica JA - haematol VL - 92 IS - 5 SE - Articles DO - 10.3324/haematol.10432 UR - https://haematologica.org/article/view/4431 SP - 605-611 AB - Background and Objectives To date, bone marrow (BM) is the most common source of cells to use in order to assess minimal residual disease (MRD) in acute myeloid leukemia (AML). In the present study, we investigated whether peripheral blood (PB) could be an alternative source of cells for monitoring MRD in AML.Design and Methods Fifty patients with AML were monitored for MRD after the achievement of complete remission. Using multiparametric flow cytometry we compared the levels of MRD in 50 and 48 pairs of BM and PB after induction and consolidation, respectively.Results After induction and consolidation therapy, the findings in BM and PB were significantly concordant (r=0.86 and 0.82, respectively, p1.5×10−4 residual leukemic cells in PB after induction had a relapse, whereas the seven patients with lower levels did not (p=0.0002). After consolidation, 38 patients had a level of MRD >1.5×10−4 and 31 (82%) had a relapse; nine out of the remaining ten patients, whose levels of MRD were below 1.5×10−4, are still relapse-free (p=0.00006). In multivariate analysis, PB MRD status at the end of consolidation was found to have a significant effect on relapse-free survival (p=0.036).Interpretation and Conclusions These preliminary results indicate that: (i) PB evaluation can integrate BM assessment for MRD detection in patients with AML; (ii) PB MRD status at the end of consolidation therapy may provide useful prognostic information. ER -