TY - JOUR AU - M Krampera, AU - O Perbellini, AU - C Vincenzi, AU - F Zampieri, AU - A Pasini, AU - MT Scupoli, AU - A Guarini, AU - MS De Propris, AU - E Coustan-Smith, AU - D Campana, AU - R Foa, AU - G Pizzolo, PY - 2006/08/01 Y2 - 2024/03/29 TI - Methodological approach to minimal residual disease detection by flow cytometry in adult B-lineage acute lymphoblastic leukemia JF - Haematologica JA - haematol VL - 91 IS - 8 SE - Articles DO - 10.3324/%x UR - https://haematologica.org/article/view/4101 SP - 1109-1112 AB - A flow cytometric approach to minimal residual disease (MRD) monitoring useful in childhood B-lineage acute lymphoblastic leukemia (ALL) is discussed here in the context of ALL in adults. Of 64 leukemia samples analyzed, 95.3% had at least one abnormal phenotype (57.3% had two or more) as compared to physiologic B-cell precursors in adult bone marrow. The method was sensitive enough to detect one leukemic cell among 10,000 normal cells in 16/19 experiments (84.2%). Blast phenotypes were stable in culture and at relapse, and were useful for MRD monitoring in patients. Marker combinations for childhood ALL are also applicable to adult cases. ER -