TY - JOUR AU - Stubbs, Matthew J. AU - Coppo, Paul AU - Cheshire, Chris AU - Veyradier, Agnès AU - Dufek, Stephanie AU - Levine, Adam P. AU - Thomas, Mari AU - Patel, Vaksha AU - Connolly, John O. AU - Hubank, Michael AU - Benhamou, Ygal AU - Galicier, Lionel AU - Poullin, Pascale AU - Kleta, Robert AU - Gale, Daniel P. AU - Stanescu, Horia AU - Scully, Marie A. PY - 2022/03/01 Y2 - 2024/03/29 TI - Identification of a novel genetic locus associated with immune-mediated thrombotic thrombocytopenic purpura JF - Haematologica JA - haematol VL - 107 IS - 3 SE - Articles DO - 10.3324/haematol.2020.274639 UR - https://haematologica.org/article/view/haematol.2020.274639 SP - 574-582 AB - Immune thrombotic thrombocytopenic purpura (iTTP) is an ultra-rare, life-threatening disorder, mediated through severe ADAMTS13 deficiency causing multi-system micro-thrombi formation, and has specific human leukocyte antigen associations. We undertook a large genome-wide association study to investigate additional genetically distinct associations in iTTP. We compared two iTTP patient cohorts with controls, following standardized genome-wide quality control procedures for single-nucleotide polymorphisms and imputed HLA types. Associations were functionally investigated using expression quantitative trait loci (eQTL), and motif binding prediction software. Independent associations consistent with previous findings in iTTP were detected at the HLA locus and in addition a novel association was detected on chromosome 3 (rs9884090, P=5.22x10-10, odds ratio 0.40) in the UK discovery cohort. Meta-analysis, including the French replication cohort, strengthened the associations. The haploblock containing rs9884090 is associated with reduced protein O-glycosyltransferase 1 (POGLUT1) expression (eQTL P<0.05), and functional annotation suggested a potential causative variant (rs71767581). This work implicates POGLUT1 in iTTP pathophysiology and suggests altered post-translational modification of its targets may influence disease susceptibility. ER -