@article{Xin Li_Zi Sheng_Yuanxin Sun_Yuanjian Wang_Miao Xu_Zhiyue Zhang_Hui Li_Linlin Shao_Yanqi Zhang_Jinming Yu_Chunhong Ma_Chengjiang Gao_Ming Hou_Heyu Ni_Jun Peng_Ji Ma_Qi Feng_2021, place={Pavia, Italy}, title={Human leukocyte antigen-G upregulates immunoglobulin-like transcripts and corrects dysfunction of immune cells in immune thrombocytopenia}, volume={106}, url={https://haematologica.org/article/view/9658}, DOI={10.3324/haematol.2018.204040}, abstractNote={Human leukocyte antigen-G is a non-classical major histocompatibility complex class I antigen with potent immune-inhibitory function. Human leukocyte antigen-G benefit patients in allotransplantation and autoimmune diseases by interacting with its receptors, immunoglobulin-like transcripts. Here we observed significantly less human leukocyte antigen-G in plasma from immune thrombocytopenia patients positive for anti-platelet autoantibodies compared with autoantibodies-negative patients or healthy controls. Besides, human leukocyte antigen-G is positively correlated with platelet counts in both patients and healthy controls. We also found less membrane-bound human leukocyte antigen-G and immunoglobulin-like transcripts on CD4+ and CD14+ cells in patients. Recombinant human leukocyte antigen-G upregulated immunoglobulin-like transcript 2 expression on CD4+ and immunoglobulin-like transcript 4 on CD14+ cells. Human leukocyte antigen-G upregulated IL-4 and IL-10, and downregulated tumor necrosis factor-α, IL-12 and IL-17 secreted by patient peripheral blood mononuclear cells, suggesting a stimulation of Th2 differentiation and downregulation of Th1 and Th17 immune response. Human leukocyte antigen-G-modulated dendritic cells from immune thrombocytopenia patients showed decreased expression of CD80 and CD86, and suppressed CD4+ T-cell proliferation compared to unmodulated cells. Moreover, human leukocyte antigen-G modulated cells from patients induced less platelet apoptosis. Human leukocyte antigen-G administration also significantly alleviated thrombocytopenia in a murine model of ITP. In conclusion, our data demonstrated that impaired expression of human leukocyte antigen-G and immunoglobulin-like transcripts is involved in the pathogenesis of immune thrombocytopenia; Recombinant human leukocyte antigen-G can correct this abnormality via upregulation of immunoglobulin-like transcripts, indicating that human leukocyte antigen-G can be a diagnostic marker and a therapeutic option for immune thrombocytopenia.}, number={3}, journal={Haematologica}, author={Xin Li and Zi Sheng and Yuanxin Sun and Yuanjian Wang and Miao Xu and Zhiyue Zhang and Hui Li and Linlin Shao and Yanqi Zhang and Jinming Yu and Chunhong Ma and Chengjiang Gao and Ming Hou and Heyu Ni and Jun Peng and Ji Ma and Qi Feng}, year={2021}, month={Mar.}, pages={770-781} }