@article{Laura A. Richards_Ashu Kumari_Kathy Knezevic_Julie AI Thoms_Georg von Jonquieres_Christine E. Napier_Zara Ali_Rosemary O’Brien_Jonathon Marks-Bluth_Michelle F. Maritz_Hilda A Pickett_Jonathan Morris_John E. Pimanda_Karen L. MacKenzie_2020, place={Pavia, Italy}, title={DKC1 is a transcriptional target of GATA1 and drives upregulation of telomerase activity in normal human erythroblasts}, volume={105}, url={https://haematologica.org/article/view/9427}, DOI={10.3324/haematol.2018.215699}, abstractNote={Telomerase is a ribonucleoprotein complex that maintains the length and integrity of telomeres, and thereby enables cellular proliferation. Understanding the regulation of telomerase in hematopoietic cells is relevant to the pathogenesis of leukemia, in which telomerase is constitutively activated, as well as bone marrow failure syndromes that feature telomerase insufficiency. Past studies showing high levels of telomerase in human erythroblasts and a prevalence of anemia in disorders of telomerase insufficiency provide the rationale for investigating telomerase regulation in erythroid cells. Here it is shown for the first time that the telomerase RNA-binding protein dyskerin (encoded by <em>DKC1</em>) is dramatically upregulated as human hematopoietic stem and progenitor cells commit to the erythroid lineage, driving an increase in telomerase activity in the presence of limiting amounts of <em>TERT</em> mRNA. It is also shown that upregulation of <em>DKC1</em> was necessary for expansion of glycophorin A<sup>+</sup> erythroblasts and sufficient to extend telomeres in erythroleukemia cells. Chromatin immunoprecipitation and reporter assays implicated GATA1-mediated transcriptional regulation of <em>DKC1</em> in the modulation of telomerase in erythroid lineage cells. Together these results describe a novel mechanism of telomerase regulation in erythroid cells which contrasts with mechanisms centered on transcriptional regulation of <em>TERT</em> that are known to operate in other cell types. This is the first study to reveal a biological context in which telomerase is upregulated by <em>DKC1</em> and to implicate <em>GATA1</em> in telomerase regulation. The results from this study are relevant to hematopoietic disorders involving <em>DKC1</em> mutations, <em>GATA1</em&gt; deregulation and/or telomerase insufficiency.}, number={6}, journal={Haematologica}, author={Laura A. Richards and Ashu Kumari and Kathy Knezevic and Julie AI Thoms and Georg von Jonquieres and Christine E. Napier and Zara Ali and Rosemary O’Brien and Jonathon Marks-Bluth and Michelle F. Maritz and Hilda A Pickett and Jonathan Morris and John E. Pimanda and Karen L. MacKenzie}, year={2020}, month={Jun.}, pages={1517-1526} }