@article{Jing Liu_Wei Liu_Yin Liu_Yang Miao_Yifan Guo_Haoyang Song_Fudi Wang_Hongyu Zhou_Tomas Ganz_Bing Yan_Sijin Liu_2019, place={Pavia, Italy}, title={New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia}, volume={104}, url={https://haematologica.org/article/view/9043}, DOI={10.3324/haematol.2018.209874}, abstractNote={Genetic iron-overload disorders, mainly hereditary hemochromatosis and untransfused β-thalassemia, affect a large population worldwide. The primary etiology of iron overload in these diseases is insufficient production of hepcidin by the liver, leading to excessive intestinal iron absorption and iron efflux from macrophages. Hepcidin agonists would therefore be expected to ameliorate iron overload in hereditary hemochromatosis and β-thalassemia. In the current study, we screened our synthetic library of 210 thiazolidinone compounds and identified three thiazolidinone compounds, 93, 156 and 165, which stimulated hepatic hepcidin production. In a hemochromatosis mouse model with hemochromatosis deficiency, the three compounds prevented the development of iron overload and elicited iron redistribution from the liver to the spleen. Moreover, these compounds also greatly ameliorated iron overload and mitigated ineffective erythropoiesis in β-thalassemic mice. Compounds 93, 156 and 165 acted by promoting SMAD1/5/8 signaling through differentially repressing ERK1/2 phosphorylation and decreasing transmembrane protease serine 6 activity. Additionally, compounds 93, 156 and 165 targeted erythroid regulators to strengthen hepcidin expression. Therefore, our hepcidin agonists induced hepcidin expression synergistically through a direct action on hepatocytes via SMAD1/5/8 signaling and an indirect action via eythroid cells. By increasing hepcidin production, thiazolidinone compounds may provide a useful alternative for the treatment of iron-overload disorders.}, number={9}, journal={Haematologica}, author={Jing Liu and Wei Liu and Yin Liu and Yang Miao and Yifan Guo and Haoyang Song and Fudi Wang and Hongyu Zhou and Tomas Ganz and Bing Yan and Sijin Liu}, year={2019}, month={Aug.}, pages={1768-1781} }