@article{Iris Z. Uras_Ruth M. Scheicher_Karoline Kollmann_Martin Glösmann_Michaela Prchal-Murphy_Anca S. Tigan_Daniela A. Fux_Sandro Altamura_Joana Neves_Martina U. Muckenthaler_Keiryn L. Bennett_Stefan Kubicek_Philip W. Hinds_Marieke von Lindern_Veronika Sexl_2017, place={Pavia, Italy}, title={Cdk6 contributes to cytoskeletal stability in erythroid cells}, volume={102}, url={https://haematologica.org/article/view/8110}, DOI={10.3324/haematol.2016.159947}, abstractNote={Mice lacking Cdk6 kinase activity suffer from mild anemia accompanied by elevated numbers of Ter119<sup>+</sup> cells in the bone marrow. The animals show hardly any alterations in erythroid development, indicating that Cdk6 is not required for proliferation and maturation of erythroid cells. There is also no difference in stress erythropoiesis following hemolysis <em>in vivo</em>. However, <em>Cdk6</em><sup>−/−</sup> erythrocytes have a shortened lifespan and are more sensitive to mechanical stress <em>in vitro</em>, suggesting differences in cytoskeletal architecture. Erythroblasts contain both Cdk4 and Cdk6, while mature erythrocytes apparently lack Cdk4 and their Cdk6 is partly associated with the cytoskeleton. We used mass spectrometry to show that Cdk6 interacts with a number of proteins involved in cytoskeleton organization. <em>Cdk6</em><sup>−/−</sup> erythroblasts show impaired F-actin formation and lower levels of gelsolin, which interacts with Cdk6. We also found that Cdk6 regulates the transcription of a panel of genes involved in actin (de-)polymerization. <em>Cdk6</em&gt;-deficient cells are sensitive to drugs that interfere with the cytoskeleton, suggesting that our findings are relevant to the treatment of patients with anemia – and may be relevant to cancer patients treated with the new generation of CDK6 inhibitors.}, number={6}, journal={Haematologica}, author={Iris Z. Uras and Ruth M. Scheicher and Karoline Kollmann and Martin Glösmann and Michaela Prchal-Murphy and Anca S. Tigan and Daniela A. Fux and Sandro Altamura and Joana Neves and Martina U. Muckenthaler and Keiryn L. Bennett and Stefan Kubicek and Philip W. Hinds and Marieke von Lindern and Veronika Sexl}, year={2017}, month={May}, pages={995-1005} }