@article{Hiroto Horiguchi_Masayoshi Kobune_Shohei Kikuchi_Masahiro Yoshida_Masaki Murata_Kazuyuki Murase_Satoshi Iyama_Kohichi Takada_Tsutomu Sato_Kaoru Ono_Akari Hashimoto_Ayumi Tatekoshi_Yusuke Kamihara_Yutaka Kawano_Koji Miyanishi_Norimasa Sawada_Junji Kato_2016, place={Pavia, Italy}, title={Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms}, volume={101}, url={https://haematologica.org/article/view/7679}, DOI={10.3324/haematol.2015.134932}, abstractNote={The failure of normal hematopoiesis is observed in myeloid neoplasms. However, the precise mechanisms governing the replacement of normal hematopoietic stem cells in their niche by myeloid neoplasm stem cells have not yet been clarified. Primary acute myeloid leukemia and myelodysplastic syndrome cells induced aberrant expression of multiple hematopoietic factors including Jagged-1, stem cell factor and angiopoietin-1 in mesenchymal stem cells even in non-contact conditions, and this abnormality was reverted by extracellular vesicle inhibition. Importantly, the transfer of myeloid neoplasm-derived extracellular vesicles reduced the hematopoietic supportive capacity of mesenchymal stem cells. Analysis of extracellular vesicle microRNA indicated that several species, including miR-7977 from acute myeloid leukemia cells, were higher than those from normal CD34<sup>+</sup> cells. Remarkably, the copy number of miR-7977 in bone marrow interstitial fluid was elevated not only in acute myeloid leukemia, but also in myelodysplastic syndrome, as compared with lymphoma without bone marrow localization. The transfection of the miR-7977 mimic reduced the expression of the posttranscriptional regulator, poly(rC) binding protein 1, in mesenchymal stem cells. Moreover, the miR-7977 mimic induced aberrant reduction of hematopoietic growth factors in mesenchymal stem cells, resulting in decreased hematopoietic-supporting capacity of bone marrow CD34<sup>+</sup&gt; cells. Furthermore, the reduction of hematopoietic growth factors including Jagged-1, stem cell factor and angiopoietin-1 were reverted by target protection of poly(rC) binding protein 1, suggesting that poly(rC) binding protein 1 could be involved in the stabilization of several growth factors. Thus, miR-7977 in extracellular vesicles may be a critical factor that induces failure of normal hematopoiesis via poly(rC) binding protein 1 suppression.}, number={4}, journal={Haematologica}, author={Hiroto Horiguchi and Masayoshi Kobune and Shohei Kikuchi and Masahiro Yoshida and Masaki Murata and Kazuyuki Murase and Satoshi Iyama and Kohichi Takada and Tsutomu Sato and Kaoru Ono and Akari Hashimoto and Ayumi Tatekoshi and Yusuke Kamihara and Yutaka Kawano and Koji Miyanishi and Norimasa Sawada and Junji Kato}, year={2016}, month={Mar.}, pages={437-447} }