@article{Yiren Xiao_Zhiwu Jiang_Yin Li_Wei Ye_Bei Jia_Minjie Zhang_Yan Xu_Donghai Wu_Liangxue Lai_Yaoyu Chen_Yingjun Chang_Xiaojun Huang_Hudan Liu_Guoliang Qing_Pentao Liu_Yangqiu Li_Bing Xu_Mei Zhong_Yao Yao_Duanqing Pei_Peng Li_2015, place={Pavia, Italy}, title={ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells}, volume={100}, url={https://haematologica.org/article/view/7366}, DOI={10.3324/haematol.2014.118612}, abstractNote={Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration.}, number={5}, journal={Haematologica}, author={Yiren Xiao and Zhiwu Jiang and Yin Li and Wei Ye and Bei Jia and Minjie Zhang and Yan Xu and Donghai Wu and Liangxue Lai and Yaoyu Chen and Yingjun Chang and Xiaojun Huang and Hudan Liu and Guoliang Qing and Pentao Liu and Yangqiu Li and Bing Xu and Mei Zhong and Yao Yao and Duanqing Pei and Peng Li}, year={2015}, month={May}, pages={585-594} }