@article{Evelien Mets_Gert Van Peer_Joni Van der Meulen_Michael Boice_Tom Taghon_Steven Goossens_Pieter Mestdagh_Yves Benoit_Barbara De Moerloose_Nadine Van Roy_Bruce Poppe_Jo Vandesompele_Hans-Guido Wendel_Pieter Van Vlierberghe_Frank Speleman_Pieter Rondou_2014, place={Pavia, Italy}, title={MicroRNA-128-3p is a novel oncomiR targeting PHF6 in T-cell acute lymphoblastic leukemia}, volume={99}, url={https://haematologica.org/article/view/7112}, DOI={10.3324/haematol.2013.099515}, abstractNote={T-cell acute lymphoblastic leukemia arises from the leukemic transformation of developing thymocytes and results from cooperative genetic lesions. Inactivation of the <em>PHF6</em> gene is frequently observed in T-cell acute lymphoblastic leukemia, suggesting an important tumor suppressive role for <em>PHF6</em> in the pathobiology of this leukemia. Although the precise function of <em>PHF6</em> is still unknown, this gene is most likely involved in chromatin regulation, a strongly emerging theme in T-cell acute lymphoblastic leukemia. In this context, our previous description of a cooperative microRNA regulatory network controlling several well-known T-cell acute lymphoblastic leukemia tumor suppressor genes, including <em>PHF6</em>, is of great importance. Given the high frequency of <em>PHF6</em> lesions in T-cell acute lymphoblastic leukemia and the integration of <em>PHF6</em> in this microRNA regulatory network, we aimed to identify novel oncogenic microRNAs in T-cell acute lymphoblastic leukemia which suppress <em>PHF6</em>. To this end, we performed an unbiased <em>PHF6</em> 3′UTR-microRNA library screen and combined the results with microRNA profiling data of samples from patients with T-cell acute lymphoblastic leukemia and normal thymocyte subsets. We selected miR-128-3p as a candidate <em>PHF6</em>-targeting, oncogenic microRNA and demonstrated regulation of <em>PHF6</em> expression upon modulation of this microRNA in T-cell acute lymphoblastic leukemia cell lines. <em>In vivo</em> evidence of an oncogenic role of this microRNA in T-cell acute lymphoblastic leukemia was obtained through accelerated leukemia onset in a NOTCH1-induced T-cell acute lymphoblastic leukemia mouse model upon miR-128-3p over-expression. We conclude that miR-128-3p is a strong novel candidate oncogenic microRNA in T-cell acute lymphoblastic leukemia which targets the <em>PHF6</em&gt; tumor suppressor gene.}, number={8}, journal={Haematologica}, author={Evelien Mets and Gert Van Peer and Joni Van der Meulen and Michael Boice and Tom Taghon and Steven Goossens and Pieter Mestdagh and Yves Benoit and Barbara De Moerloose and Nadine Van Roy and Bruce Poppe and Jo Vandesompele and Hans-Guido Wendel and Pieter Van Vlierberghe and Frank Speleman and Pieter Rondou}, year={2014}, month={Jul.}, pages={1326-1333} }