@article{Boris Kovacic_Andrea Hoelbl-Kovacic_Katrin M. Fischhuber_Nicole R. Leitner_Dagmar Gotthardt_Emilio Casanova_Veronika Sexl_Mathias Müller_2014, place={Pavia, Italy}, title={Lactotransferrin-Cre reporter mice trace neutrophils, monocytes/macrophages and distinct subtypes of dendritic cells}, volume={99}, url={https://haematologica.org/article/view/7041}, DOI={10.3324/haematol.2013.097154}, abstractNote={Considerable effort has been expended to identify genes that account for myeloid lineage commitment and development. However, currently available non-invasive mouse models utilize myeloid-specific reporters that are significantly expressed in hematopoietic stem cells as well as lymphoid compartments. Here, we describe a myeloid-specific marker that is not shared by any other lineage. We show that <em>lactotransferrin</em> mRNA is expressed by Gr-1<sup>+</sup>/CD11b<sup>+</sup> cells in the bone marrow, as opposed to hematopoietic stem cells or any peripheral cell population. To follow the progeny of <em>lactotransferrin</em>-expressing bone marrow cells, we generated a mouse model in which a reporter gene is irreversibly activated from the <em>lactotransferrin</em>-promoter. We found that <em>lactotransferrin</em>-reporter labels a majority of neutrophils, monocytes, macrophages and distinct subtypes of dendritic cells, while excluding T, B, natural killer cells, interferon-producing killer dendritic cells, plasmacytoid dendritic cells, erythrocytes and eosinophils. <em>Lactotransferrin</em>-reporter<sup>−</sup> bone marrow cells retain lymphoid, erythroid and long-term repopulating potential, while <em>lactotransferrin</em>-reporter<sup>+</sup> bone marrow cells confer only myeloid, but not lymphoid potential. We conclude that <em>lactotransferrin</em&gt; represents a late stage differentiation marker of neutrophils, macrophages and distinct subtypes of dendritic cells.}, number={6}, journal={Haematologica}, author={Boris Kovacic and Andrea Hoelbl-Kovacic and Katrin M. Fischhuber and Nicole R. Leitner and Dagmar Gotthardt and Emilio Casanova and Veronika Sexl and Mathias Müller}, year={2014}, month={May}, pages={1006-1015} }