@article{Bettina Bisig_Aurélien de Reyniès_Christophe Bonnet_Pierre Sujobert_David S. Rickman_Teresa Marafioti_Georges Delsol_Laurence Lamant_Philippe Gaulard_Laurence de Leval_2013, place={Pavia, Italy}, title={CD30-positive peripheral T-cell lymphomas share molecular and phenotypic features}, volume={98}, url={https://haematologica.org/article/view/6751}, DOI={10.3324/haematol.2012.081935}, abstractNote={Peripheral T-cell lymphoma, not otherwise specified is a heterogeneous group of aggressive neoplasms with indistinct borders. By gene expression profiling we previously reported unsupervised clusters of peripheral T-cell lymphomas, not otherwise specified correlating with CD30 expression. In this work we extended the analysis of peripheral T-cell lymphoma molecular profiles to prototypical CD30<sup>+</sup> peripheral T-cell lymphomas (anaplastic large cell lymphomas), and validated mRNA expression profiles at the protein level. Existing transcriptomic datasets from peripheral T-cell lymphomas, not otherwise specified and anaplastic large cell lymphomas were reanalyzed. Twenty-one markers were selected for immunohistochemical validation on 80 peripheral T-cell lymphoma samples (not otherwise specified, CD30<sup>+</sup> and CD30<sup>−</sup>; anaplastic large cell lymphomas, ALK<sup>+</sup> and ALK<sup>−</sup>), and differences between subgroups were assessed. Clinical follow-up was recorded. Compared to CD30<sup>−</sup> tumors, CD30<sup>+</sup> peripheral T-cell lymphomas, not otherwise specified were significantly enriched in ALK<sup>−</sup> anaplastic large cell lymphoma-related genes. By immunohistochemistry, CD30<sup>+</sup> peripheral T-cell lymphomas, not otherwise specified differed significantly from CD30<sup>−</sup> samples [down-regulated expression of T-cell receptor-associated proximal tyrosine kinases (Lck, Fyn, Itk) and of proteins involved in T-cell differentiation/activation (CD69, ICOS, CD52, NFATc2); upregulation of JunB and MUM1], while overlapping with anaplastic large cell lymphomas. CD30<sup>−</sup> peripheral T-cell lymphomas, not otherwise specified tended to have an inferior clinical outcome compared to the CD30<sup>+</sup> subgroups. In conclusion, we show molecular and phenotypic features common to CD30<sup>+</sup> peripheral T-cell lymphomas, and significant differences between CD30<sup>−</sup> and CD30<sup>+</sup&gt; peripheral T-cell lymphomas, not otherwise specified, suggesting that CD30 expression might delineate two biologically distinct subgroups.}, number={8}, journal={Haematologica}, author={Bettina Bisig and Aurélien de Reyniès and Christophe Bonnet and Pierre Sujobert and David S. Rickman and Teresa Marafioti and Georges Delsol and Laurence Lamant and Philippe Gaulard and Laurence de Leval}, year={2013}, month={Jul.}, pages={1250-1258} }