@article{Eva Barragán_Pau Montesinos_Mireia Camos_Marcos González_Maria J. Calasanz_José Román-Gómez_Maria T. Gómez-Casares_Rosa Ayala_Javier López_Óscar Fuster_Dolors Colomer_Carmen Chillón_María J. Larrayoz_Pedro Sánchez-Godoy_José González-Campos_Félix Manso_Maria L. Amador_Edo Vellenga_Bob Lowenberg_Miguel A. Sanz_2011, place={Pavia, Italy}, title={Prognostic value of FLT3 mutations in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and anthracycline monochemotherapy}, volume={96}, url={https://haematologica.org/article/view/6094}, DOI={10.3324/haematol.2011.044933}, abstractNote={<strong>Background</strong> <em>Fms-like tyrosine kinase-3</em> (<em>FLT3</em>) gene mutations are frequent in acute promyelocytic leukemia but their prognostic value is not well established.<strong>Design and Methods</strong> We evaluated <em>FLT3</em>-internal tandem duplication and <em>FLT3</em>-D835 mutations in patients treated with all-trans retinoic acid and anthracycline-based chemotherapy enrolled in two subsequent trials of the <em>Programa de Estudio y Tratamiento de las Hemopatías Malignas</em> (PETHEMA) and <em>Hemato-Oncologie voor Volwassenen Nederland</em> (HOVON) groups between 1996 and 2005.<strong>Results</strong> <em>FLT3</em>-internal tandem duplication and <em>FLT3</em>-D835 mutation status was available for 306 (41%) and 213 (29%) patients, respectively. Sixty-eight (22%) and 20 (9%) patients had internal tandem duplication and D835 mutations, respectively. Internal tandem duplication was correlated with higher white blood cell and blast counts, lactate dehydrogenase, relapse-risk score, fever, hemorrhage, coagulopathy, BCR3 isoform, M3 variant subtype, and expression of CD2, CD34, human leukocyte antigen-DR, and CD11b surface antigens. The <em>FLT3</em>-D835 mutation was not significantly associated with any clinical or biological characteristic. Univariate analysis showed higher relapse and lower survival rates in patients with a <em>FLT3</em>-internal tandem duplication, while no impact was observed in relation to <em>FLT3</em>-D835. The prognostic value of the <em>FLT3</em>-internal tandem duplication was not retained in the multivariate analysis.<strong>Conclusions</strong> <em>FLT3-</em&gt;internal tandem duplication mutations are associated with several hematologic features in acute promyelocytic leukemia, in particular with high white blood cell counts, but we were unable to demonstrate an independent prognostic value in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and anthracycline-based regimens.}, number={10}, journal={Haematologica}, author={Eva Barragán and Pau Montesinos and Mireia Camos and Marcos González and Maria J. Calasanz and José Román-Gómez and Maria T. Gómez-Casares and Rosa Ayala and Javier López and Óscar Fuster and Dolors Colomer and Carmen Chillón and María J. Larrayoz and Pedro Sánchez-Godoy and José González-Campos and Félix Manso and Maria L. Amador and Edo Vellenga and Bob Lowenberg and Miguel A. Sanz}, year={2011}, month={Oct.}, pages={1470-1477} }