@article{Andrew McLarnon_Karen P. Piper_Oliver C. Goodyear_Julie M. Arrazi_Premini Mahendra_Mark Cook_Fiona Clark_Guy Pratt_Charles Craddock_Paul. A.H. Moss_2010, place={Pavia, Italy}, title={CD8+ T-cell immunity against cancer-testis antigens develops following allogeneic stem cell transplantation and reveals a potential mechanism for the graft-versus-leukemia effect}, volume={95}, url={https://haematologica.org/article/view/5723}, DOI={10.3324/haematol.2009.019539}, abstractNote={<strong>Background</strong> Allogeneic stem cell transplantation is associated with a powerful ‘graft-<em>versus</em>-leukemia’ effect that is generally considered to result from an alloreactive T-cell immune response. However, disease remission can also be observed after syngeneic transplantation and we investigated whether a T-cell immune response to cancer-testis antigens can be detected in patients in the post-transplant period.<strong>Design and Methods</strong> The T-cell immune response against cancer-testis antigens was studied in a cohort of 41 patients who underwent allogeneic stem cell transplantation for the management of acute myeloid leukemia or multiple myeloma. The cytokine secretion assay was combined with magnetic selection to allow detection of an interferon-γ-secreting T-cell response to a panel of cancer-testis antigen peptides.<strong>Results</strong> A cancer-testis antigen-specific CD8<sup>+</sup> T-cell immune response was observed in the peripheral blood of five patients with an average magnitude of 0.045% of the CD8<sup>+</sup> T-cell repertoire. Four of these patients had undergone reduced intensity conditioning transplantation with alemtuzumab for the treatment of acute myeloid leukemia and three remain in long-term remission. T-cell immunity was focused against peptides derived from MAGE proteins and was markedly increased within the bone marrow.<strong>Conclusions</strong> Functional cancer-testis antigen-specific CD8<sup>+</sup> T-cell immune responses develop in the early period following reduced intensity allogeneic stem cell transplantation and are preferentially localized to bone marrow. These immune responses are likely to contribute to the cellular basis of the graft-<em>versus</em&gt;-leukemia effect.}, number={9}, journal={Haematologica}, author={Andrew McLarnon and Karen P. Piper and Oliver C. Goodyear and Julie M. Arrazi and Premini Mahendra and Mark Cook and Fiona Clark and Guy Pratt and Charles Craddock and Paul. A.H. Moss}, year={2010}, month={Aug.}, pages={1572-1578} }