@article{Judith Dierlamm_Eva M. Murga Penas_Stefan Bentink_Swen Wessendorf_Hilmar Berger_Michael Hummel_Wolfram Klapper_Dido Lenze_Andreas Rosenwald_Eugenia Haralambieva_German Ott_Sergio B. Cogliatti_Peter Möller_Carsten Schwaenen_Harald Stein_Markus Löffler_Rainer Spang_Lorenz Trümper_Reiner Siebert_for the Deutsche Krebshilfe Network Project “Molecular Mechanisms in Malignant Lymphomas”_2008, place={Pavia, Italy}, title={Gain of chromosome region 18q21 including the MALT1 gene is associated with the activated B-cell-like gene expression subtype and increased BCL2 gene dosage and protein expression in diffuse large B-cell lymphoma}, volume={93}, url={https://haematologica.org/article/view/4841}, DOI={10.3324/haematol.12057}, abstractNote={<strong>Background</strong> The aim of this study was to determine the impact of a gain of the <em>MALT1</em> gene on gene expression and clinical parameters in diffuse large B-cell lymphoma.<strong>Design and Methods</strong> We analyzed 116 cases of diffuse large B-cell lymphoma by fluorescence <em>in situ</em> hybridization, array-based comparative genomic hybridization, and transcriptional profiling.<strong>Results</strong> A gain of 18q21 including <em>MALT1</em> was detected in 44 cases (38%) and was accompanied by a gain of <em>BCL2</em> in 43 cases. All cases with a 18q21/<em>MALT1</em> gain showed BCL2 protein whereas 79% in the group without a 18q21/<em>MALT1</em> gain did so (<em>p</em><0.001). Cases with 18q21/<em>MALT1</em> gain more frequently showed an activated B-cell-like (ABC) gene expression signature (65%) than a germinal center B-cell-like (GCB) one (23%) (<em>p</em><0.001). Ninety-eight genes including <em>MALT1</em>, <em>BCL2</em>, and some selected nuclear factor-κB target genes were differentially expressed between the two genetic groups of diffuse large B-cell lymphoma. By global testing of each chromosome, we identified 33 genes, all located on chromosome 18q, which were differentially expressed between the two genetic groups independently of the ABC/GCB status. In multivariate analysis, the 18q21/<em>MALT1</em> status represented an independent negative prognostic factor for overall survival (<em>p</em>=0.03).<strong>Conclusions</strong> In diffuse large B-cell lymphoma, gain of 18q21 including <em>MALT1</em> is significantly associated with differential expression of genes located on 18q, the ABC gene expression subtype, increased <em>BCL2</em&gt; gene and protein expression and might indicate an unfavorable prognosis.}, number={5}, journal={Haematologica}, author={Judith Dierlamm and Eva M. Murga Penas and Stefan Bentink and Swen Wessendorf and Hilmar Berger and Michael Hummel and Wolfram Klapper and Dido Lenze and Andreas Rosenwald and Eugenia Haralambieva and German Ott and Sergio B. Cogliatti and Peter Möller and Carsten Schwaenen and Harald Stein and Markus Löffler and Rainer Spang and Lorenz Trümper and Reiner Siebert and for the Deutsche Krebshilfe Network Project “Molecular Mechanisms in Malignant Lymphomas”}, year={2008}, month={Apr.}, pages={688-696} }