@article{Eva Zetterberg_Alessandro M. Vannucchi_Anna Rita Migliaccio_William Vainchenker_Micheline Tulliez_Renée Dickie_Hans Hasselbalch_Rick Rogers_Jan Palmblad_2007, place={Pavia, Italy}, title={Pericyte coverage of abnormal blood vessels in myelofibrotic bone marrows}, volume={92}, url={https://haematologica.org/article/view/4430}, DOI={10.3324/haematol.11013}, abstractNote={<strong>Background and Objectives</strong> Myelofibrotic bone marrow displays abnormal angiogenesis but the pathogenic mechanisms of this are poorly understood. Since pericyte abnormalities are described on solid tumor vessels we studied whether vessel morphology and pericyte coverage in bone marrow samples from patients with myelofibrosis differed from that in samples from controls.<strong>Design and Methods</strong> We assessed the microvascular density (MVD), vessel morphology and pericyte coverage in bone marrows from 19 myelofibrosis patients and nine controls. We also studied the same parameters in two mouse models of myelofibrosis, with genetic alterations affecting megakaryocyte differentiation (i.e. one model with low GATA-1 expression and the other with over-expression of thrombopoietin).<strong>Results</strong> In myelofibrotic marrows, MVD was 3.8-fold greater than in controls (<em>p</em><0.001) and vessels displayed 5.9-fold larger mean perimeters (<em>p</em><0.001). MVD was 1.8-fold greater in <em>JAK2</em> V617F-positive than in negative patients (<em>p</em>=0.026). Moreover, 92±11 % of vessels in patients with myelofibrosis were pericyte-coated but only 51±20 % of vessels in controls (<em>p</em><0.001). In the two mouse models of myelofibrosis caused by targeting megakaryocytopoesis, wide, pericyte-coated and morphologically aberrant vessels were detected. MVD was significantly greater in bone marrow and spleen samples from animals with myelofibrosis than in wild-type mice.<strong>Interpretation and Conclusions</strong&gt; We conclude that angiogenesis is similarly abnormal in human and murine myelofibrosis with intense pericyte coating, presumably related to abnormal megakaryocytopoiesis.}, number={5}, journal={Haematologica}, author={Eva Zetterberg and Alessandro M. Vannucchi and Anna Rita Migliaccio and William Vainchenker and Micheline Tulliez and Renée Dickie and Hans Hasselbalch and Rick Rogers and Jan Palmblad}, year={2007}, month={May}, pages={597-604} }