@article{K Johrens_Y Shimizu_I Anagnostopoulos_S Schiffmann_E Tiacci_B Falini_H Stein_2005, place={Pavia, Italy}, title={T-bet-positive and IRTA1-positive monocytoid B cells differ from marginal zone B cells and epithelial-associated B cells in their antigen profile and topographical distribution}, volume={90}, url={https://haematologica.org/article/view/3627}, DOI={10.3324/%x}, abstractNote={BACKGROUND AND OBJECTIVES: It remains controversial whether splenic and nodal marginal zone B cells, monocytoid -, and epithelial-associated B cells represent separate B-cell subsets or just variants of the same population. To clarify this issue we studied the antigen profile and topographical distribution of these cell types. DESIGN AND METHODS: We studied samples of toxoplasmic lymphadenopathy, lymph nodes with a developed marginal zone, and hyperplastic palatine tonsils. Deparaffinized sections were subjected to antigen-retrieval pre-treatment then incubated with appropriate antibodies. The bound antibodies were made visible using the alkaline phosphatase anti-alkaline phosphatase method with FastRed as the chromogen. RESULTS: We found that i) nodal marginal zone B cells have a similar immunophenotype and topographical distribution to their splenic counterparts, ii) monocytoid B cells differ in their antigen profile (presence of T-bet, IRTA1, CD75, CD45RA, absence of BCL-2 , CD21, CD27) from splenic and nodal marginal zone B cells and more closely resemble epithelial-associated B cells (presence of IRTA-1, CD45RA, partial expression of T-bet, CD75, absence of CD21, CD27). Monocytoid B cells were mostly not found in nodal marginal zones when a marginal zone was developed, but were seen in areas adjacent to marginal zones and occasionally in germinal centers. INTERPRETATION AND CONCLUSIONS: Collectively, our results indicate that monocytoid B cells represent a distinct differentiated B-cell subset, and provide a solid basis for isolation and functional investigations of the cell types studied, and for revising the hitherto inadequate classification of nodal marginal zone lymphomas.}, number={8}, journal={Haematologica}, author={K Johrens and Y Shimizu and I Anagnostopoulos and S Schiffmann and E Tiacci and B Falini and H Stein}, year={2005}, month={Aug.}, pages={1070-1077} }