@article{Tassi_Noviello_De Simone_Lupo-Stanghellini_Doglio_Serio_Abbati_Beretta_Valtolina_Oliveira_Racca_Campodonico_Ruggiero_Clerici_Giglio_Lorentino_Dvir_Xue_Farina_Oltolini_Manfredi_Vago_Corti_Bernardi_Clementi_Brix_Ciceri_Peccatori_Greco_Bonini_2023, place={Pavia, Italy}, title={Cytomegalovirus-specific T cells restricted for shared and donor human leukocyte antigens differentially impact on cytomegalovirus reactivation risk after allogeneic hematopoietic stem cell transplantation}, volume={108}, url={https://haematologica.org/article/view/haematol.2022.280685}, DOI={10.3324/haematol.2022.280685}, abstractNote={<p>After allogeneic hematopoietic stem cell transplantation (HSCT), the emergence of circulating cytomegalovirus (CMV)- specific T cells correlates with protection from CMV reactivation, an important risk factor for non-relapse mortality. However, functional assays measuring CMV-specific cells are time-consuming and often inaccurate at early time-points. We report the results of a prospective single-center, non-interventional study that identified the enumeration of Dextramerpositive CMV-specific lymphocytes as a reliable and early predictor of viral reactivation. We longitudinally monitored 75 consecutive patients for 1 year after allogeneic HSCT (n=630 samples). The presence of ≥0.5 CMV-specific CD8+ cells/mL at day +45 was an independent protective factor from subsequent clinically relevant reactivation in univariate (P&lt;0.01) and multivariate (P&lt;0.05) analyses. Dextramer quantification correlated with functional assays measuring interferon-γ production, and allowed earlier identification of high-risk patients. In mismatched transplants, the comparative analysis of lymphocytes restricted by shared, donor- and host-specific HLA revealed the dominant role of thymic-independent CMV-specific reconstitution. Shared and donor-restricted CMV-specific T cells reconstituted with similar kinetics in recipients of CMV-seropositive donors, while donor-restricted T-cell reconstitution from CMV-seronegative grafts was impaired, indicating that in primary immunological responses the emergence of viral-specific T cells is largely sustained by antigen encounter on host infected cells rather than by cross-priming/presentation by non-infected donor-derived antigen-presenting cells. Multiparametric flow cytometry and high-dimensional analysis showed that shared-restricted CMV-specific lymphocytes display a more differentiated phenotype and increased persistence than donor-restricted counterparts. In this study, monitoring CMV-specific cells by Dextramer assay after allogeneic HSCT shed light on mechanisms of immune reconstitution and enabled risk stratification of patients, which could improve the clinical management of post-transplant CMV reactivations.</p&gt;}, number={6}, journal={Haematologica}, author={Tassi, Elena and Noviello, Maddalena and De Simone, Pantaleo and Lupo-Stanghellini, Maria T. and Doglio, Matteo and Serio, Francesca and Abbati, Danilo and Beretta, Valeria and Valtolina, Veronica and Oliveira, Giacomo and Racca, Sara and Campodonico, Edoardo and Ruggiero, Eliana and Clerici, Daniela and Giglio, Fabio and Lorentino, Francesca and Dvir, Roee and Xue, Elisabetta and Farina, Francesca and Oltolini, Chiara and Manfredi, Francesco and Vago, Luca and Corti, Consuelo and Bernardi, Massimo and Clementi, Massimo and Brix, Liselotte and Ciceri, Fabio and Peccatori, Jacopo and Greco, Raffaella and Bonini, Chiara}, year={2023}, month={Jun.}, pages={1530-1543} }