@article{Goodings_Zhao_McKinney-Freeman_Zhang_Yang_2023, place={Pavia, Italy}, title={<i>ARID5B</i> influences B-cell development and function in mouse}, volume={108}, url={https://haematologica.org/article/view/haematol.2022.281157}, DOI={10.3324/haematol.2022.281157}, abstractNote={<p>There is growing evidence for an inherited basis of susceptibility to childhood acute lymphoblastic leukemia. Genomewide association studies by us and others have identified non-coding acute lymphoblastic leukemia risk variants at the ARID5B gene locus, but the molecular mechanisms linking ARID5B to normal and malignant hematopoiesis remain largely unknown. Using a Vav1-driven transgenic mouse model, we characterized the role of Arid5b in hematopoiesis in vivo. Arid5b overexpression resulted in a dramatic reduction in the proportion of circulating B cells, immature, and mature Bcell fractions in the peripheral blood and the bone marrow, and also a decrease of follicular B cells in the spleen. There were significant defects in B-cell activation upon Arid5b overexpression in vitro with hyperactivation of B-cell receptor signaling at baseline. In addition, increased mitochondrial oxygen consumption rate of naïve or stimulated B cells of Arid5b-overexpressing mice was observed, compared to the rate of wild-type counterparts. Taken together, our results indicate that ARID5B may play an important role in B-cell development and function.</p&gt;}, number={2}, journal={Haematologica}, author={Goodings, Charnise and Zhao, Xujie and McKinney-Freeman, Shannon and Zhang, Hui and Yang, Jun J.}, year={2023}, month={Feb.}, pages={502-512} }