@article{Wang_Xia_Weng_Wang_Dong_Hao_Dong_Liu_Liu_Geng_Guan_Du_Cheng_Cheng_Wang_2022, place={Pavia, Italy}, title={Loss of <I>Nupr1</I> promotes engraftment by tuning the quiescence threshold of hematopoietic stem cells via regulation of the p53-checkpoint pathway}, volume={107}, url={https://haematologica.org/article/view/haematol.2019.239186}, DOI={10.3324/haematol.2019.239186}, abstractNote={<p>Hematopoietic stem cells (HSC) are dominantly quiescent under homeostasis, which is a key mechanism of maintaining the HSC pool for life-long hematopoiesis. Dormant HSC are poised to be immediately activated in certain conditions and can return to quiescence after homeostasis has been regained. At present, the molecular networks of regulating the threshold of HSC dormancy, if existing, remain largely unknown. Here, we show that deletion of Nupr1, a gene preferentially expressed in HSC, activated quiescent HSC under homeostasis, which conferred a competitive engraftment advantage for these HSC without compromising their stemness or multi-lineage differentiation capacity in serial transplantation settings. Following an expansion protocol, the Nupr1-/- HSC proliferated more robustly than their wild-type counterparts in vitro. Nupr1 inhibits the expression of p53 and rescue of this inhibition offsets the engraftment advantage. Our data reveal a new role for Nupr1 as a regulator of HSC quiescence, which provides insights for accelerating the engraftment efficacy of HSC transplantation by targeting the HSC quiescence-controlling network.</p&gt;}, number={1}, journal={Haematologica}, author={Wang, Tongjie and Xia, Chengxiang and Weng, Qitong and Wang, Kaitao and Dong, Yong and Hao, Sha and Dong, Fang and Liu, Xiaofei and Liu, Lijuan and Geng, Yang and Guan, Yuxian and Du, Juan and Cheng, Tao and Cheng, Hui and Wang, Jinyong}, year={2022}, month={Jan.}, pages={154-166} }